GeneNetwork offers unprecedented access to the data of thousands of QTL and GWAS experiments executed in the last decennium. These datasets include mouse, rat, arabidopsis and even human data. Next to having a browser-based user interface for accessing this data, our goal is to present all data also through a REST API, so that it can be used by anyone who has access to, for example, R and Python. Next to R/qtl-style CSV, most of the GN API outputs JSON. JSON can be parsed by JSON parsers, available in most programming languages.
See if the server is live
curl "http://test-gn2.genenetwork.org/api_pre1/"
{"version":"0.50-pre1","I am":"genenetwork"}or with wget
wget -qO - "http://test-gn2.genenetwork.org/api_pre1/"
{"version":"0.50-pre1","I am":"genenetwork"}At this point almost all mouse BXD data is available through the REST API (some 3,500 datasets!). We are expanding to all other non-human datasets soon. Please contact us if you are interested in more data.
When you use this data for a publication make sure what the status is by contacting the authors/owners of the dataset. Check also http://genenetwork.org/statusandContact.html. In case you need clarification please contact the GeneNetwork project.
curl "http://test-gn2.genenetwork.org/api_pre1/species"
[[1,"mouse","Mus musculus"],[2,"rat","Rattus norvegicus"],[3,"arabidopsis","Arabidopsis thaliana"],[4,"human","Homo sapiens"],[5,"barley","Hordeum vulgare"],[6,"drosophila","Drosophila melanogaster"],[7,"macaque monkey","Macaca mulatta"],[8,"soybean","Soybean"],[9,"tomato","Tomato"]]To get information on the BXD cross
curl "http://test-gn2.genenetwork.org/api_pre1/group/BXD.json"
{"species_id":1,"species":"mouse","mapping_method_id":1,"group_id":1,"group":"BXD","genetic_type":"riset","chr_info":[["1",197195432],["2",181748087],["3",159599783],["4",155630120],["5",152537259],["6",149517037],["7",152524553],["8",131738871],["9",124076172],["10",129993255],["11",121843856],["12",121257530],["13",120284312],["14",125194864],["15",103494974],["16",98319150],["17",95272651],["18",90772031],["19",61342430],["X",166650296]]}to list all available datasets for the BXD
curl "http://test-gn2.genenetwork.org/api_pre1/datasets/BXD"
[[7,"HC_U_0304_R","GNF Stem Cells U74Av2 (Mar04) RMA"],[9,"CB_M_1003_M","SJUT Cerebellum mRNA M430 (Oct03) MAS5"],[42,"CB_M_0204_P","INIA Brain mRNA M430 (Feb04) PDNN"],[44,"CB_M_1004_M","SJUT Cerebellum mRNA M430 (Oct04) MAS5"]
...To get information on a dataset, e.g. HC_U_0304_R
curl "http://test-gn2.genenetwork.org/api_pre1/dataset/HC_U_0304_R.json"
{"tissue_id":3,"tissue":"Hematopoietic Cells mRNA","short_name":"GNF Stem Cells U74Av2 (Mar04) RMA","public":2,"name":"HC_U_0304_R","id":7,"full_name":"GNF Stem Cells U74Av2 (Mar04) RMA","dataset":"probeset","data_scale":"log2","confidential":0}or through its id (note the setting of "dataset")
curl "http://test-gn2.genenetwork.org/api_pre1/dataset/10001.json"
{"year":"2001","title":"Genetic control of the mouse cerebellum: identification of quantitative trait loci modulating size and architecture","pmid":11438585,"name":"CBLWT2","id":10001,"descr":"Central nervous system, morphology: Cerebellum weight, whole, bilateral in adults of both sexes [mg]","dataset":"phenotype"}or for a high-throughput set:
curl "http://test-gn2.genenetwork.org/api_pre1/dataset/7.json"
{"tissue_id":3,"tissue":"Hematopoietic Cells mRNA","short_name":"GNF Stem Cells U74Av2 (Mar04) RMA","public":2,"name":"HC_U_0304_R","id":7,"full_name":"GNF Stem Cells U74Av2 (Mar04) RMA","dataset":"probeset","data_scale":"log2","confidential":0}Phenotype datasets are 'classic' QTL datasets with individuals (strains) and measured traits - distinguishable on the 'dataset=phenotype' field in the dataset record above.
Fetch the measurements with
curl "http://test-gn2.genenetwork.org/api_pre1/trait/10001.json"
[[4,"BXD1",61.400001525878906,2.380000114440918],[5,"BXD2",49.0,1.25],[6,"BXD5",62.5,2.319999933242798],[7,"BXD6",53.099998474121094,1.2200000286102295],[8,"BXD8",59.099998474121094,2.069999933242798],[9,"BXD9",53.900001525878906,1.0499999523162842],[10,"BXD11",53.099998474121094,1.100000023841858],[11,"BXD12",45.900001525878906,1.090000033378601],[12,"BXD13",48.400001525878906,1.6299999952316284],[13,"BXD14",49.400001525878906,0.4399999976158142],[14,"BXD15",47.400001525878906,1.149999976158142],[15,"BXD16",56.29999923706055,1.2100000381469727],[16,"BXD18",53.599998474121094,1.440000057220459],[17,"BXD19",50.099998474121094,1.4199999570846558],[18,"BXD20",48.20000076293945,1.6699999570846558],[19,"BXD21",50.599998474121094,1.309999942779541],[20,"BXD22",53.79999923706055,1.5099999904632568],[21,"BXD23",48.599998474121094,1.0299999713897705],[22,"BXD24",54.900001525878906,1.9199999570846558],[23,"BXD25",49.599998474121094,0.8100000023841858],[24,"BXD27",47.400001525878906,2.25],[25,"BXD28",51.5,0.8700000047683716],[26,"BXD29",50.20000076293945,0.5600000023841858],[27,"BXD30",53.599998474121094,1.1399999856948853],[28,"BXD31",49.70000076293945,0.9100000262260437],[29,"BXD32",56.0,1.190000057220459],[30,"BXD33",52.099998474121094,0.6600000262260437],[31,"BXD34",53.70000076293945,1.2200000286102295],[32,"BXD35",49.70000076293945,2.0299999713897705],[33,"BXD36",44.5,0.7300000190734863],[35,"BXD38",51.099998474121094,1.7899999618530273],[36,"BXD39",54.900001525878906,0.8700000047683716],[37,"BXD40",49.900001525878906,1.1299999952316284],[39,"BXD42",59.400001525878906,0.949999988079071]]which contains the records for [strain_id,strain,value,s.e.] for dataset 10001.
Or from the dataset name
curl "http://test-gn2.genenetwork.org/api_pre1/trait/CBLDT2.json"
(nyi)GN2 can also return the phenotypes in the R/qtl2 CSV data format as described here.
curl "http://test-gn2.genenetwork.org/api_pre1/trait/10001.csv"
(nyi)get dataset phenotype information for a range (here mRNA probesets) - note this only works for datasets that have the 'dataset=probeset' attribute.
curl "http://test-gn2.genenetwork.org/api_pre1/phenotypes/HC_U_0304_R.json?start=100&stop=101"
[{"symbol":"0610005C13Rik","p_value":0.666,"name_id":104617,"name":"104617_at","mean":8.165623333333329,"locus":"mCV25433152","chr":7,"additive":-0.0489058035714287,"Mb":52.823543,"MAX_LRS":9.99712881751494},
{"symbol":"0610006I08Rik","p_value":0.914,"name_id":96017,"name":"96017_at","mean":10.4658333333333,"locus":"CEL-3_23204282","chr":19,"additive":0.0437053571428568,"Mb":8.845681,"MAX_LRS":7.76436750913729}]Get the actual trait values or measurements of a sample with the stderr (in this dataset stderr is missing, so null)
curl "http://test-gn2.genenetwork.org/api_pre1/trait/HC_U_0304_R/104617_at.json"
[[4,"BXD1",8.049,null],[5,"BXD2",8.116,null],[6,"BXD5",8.064,null],[7,"BXD6",8.17,null],[8,"BXD8",8.067,null],[9,"BXD9",8.105,null],[10,"BXD11",8.317,null],[11,"BXD12",8.127,null],[13,"BXD14",8.135,null],[14,"BXD15",8.253,null],[15,"BXD16",8.335,null],[16,"BXD18",8.208,null],[17,"BXD19",8.239,null],[19,"BXD21",8.158,null],[20,"BXD22",8.226,null],[22,"BXD24",8.043,null],[23,"BXD25",7.947,null],[24,"BXD27",8.201,null],[25,"BXD28",8.068,null],[26,"BXD29",8.238,null],[27,"BXD30",8.223,null],[28,"BXD31",8.119,null],[29,"BXD32",8.039,null],[30,"BXD33",8.271,null],[31,"BXD34",8.314,null],[33,"BXD36",8.194,null],[35,"BXD38",8.163,null],[36,"BXD39",8.197,null],[37,"BXD40",8.15,null],[39,"BXD42",8.231,null]]here another example of a sample that includes parent values and stderr:
curl "http://test-gn2.genenetwork.org/api_pre1/trait/HC_M2_0606_P/1443823_s_at.json"
[[1,"B6D2F1",15.251,0.136],[2,"C57BL/6J",15.626,0.28],[3,"DBA/2J",14.716,0.26],[4,"BXD1",15.198,0.153],[5,"BXD2",14.918,0.023],[6,"BXD5",15.057,0.273],[7,"BXD6",15.232,0.107],[8,"BXD8",14.968,0.189],[9,"BXD9",14.87,0.454],[10,"BXD11",15.084,0.082],[11,"BXD12",15.192,0.298],[12,"BXD13",14.924,0.33],[14,"BXD15",15.343,0.34],[15,"BXD16",15.226,0.071],[17,"BXD19",15.364,0.074],[18,"BXD20",15.36,0.103],[19,"BXD21",14.792,0.911],...GN2 can also return the phenotypes in the R/qtl2 CSV data format as described here.
curl "http://test-gn2.genenetwork.org/api_pre1/trait/HC_U_0304_R/104617_at.csv"
id,value
4,8.049
5,8.116
6,8.064
7,8.17
8,8.067
9,8.105
10,8.317Note that the identifiers match the strain ID. Getting information on these, as well as the individual genotypes is WIP.
GN2 returns the genotypes in the R/qtl2 CSV data format as described here. Note that we offer genotypes in the transposed form (row = marker).
curl "http://test-gn2.genenetwork.org/api_pre1/genotype/mouse/BXD/geno.csv"returns
BXD5,BXD6,BXD8,BXD9,BXD11,BXD12,BXD13,BXD14,BXD15,BXD16,BXD18,BXD19,BXD20,BXD21,BXD22,BXD23,BXD24a,BXD24,BXD25,BXD27,BXD28,BXD29,BXD30,BXD31,BXD32,BXD33,BXD34,BXD35,BXD36,BXD37,BXD38,BXD39,BXD40,BXD41,BXD42,BXD43,BXD44,BXD45,BXD48,BXD49,BXD50,BXD51,BXD52,BXD53,BXD54,BXD55,BXD56,BXD59,BXD60,BXD61,BXD62,BXD63,BXD64,BXD65,BXD66,BXD67,BXD68,BXD69,BXD70,BXD71,BXD72,BXD73,BXD74,BXD75,BXD76,BXD77,BXD78,BXD79,BXD80,BXD81,BXD83,BXD84,BXD85,BXD86,BXD87,BXD88,BXD89,BXD90,BXD91,BXD92,BXD93,BXD94,BXD95,BXD96,BXD97,BXD98,BXD99,BXD100,BXD101,BXD102,BXD103
rs6269442,B,B,D,D,D,B,B,D,B,B,D,D,B,D,D,D,D,B,B,B,D,B,D,D,B,B,B,B,B,B,B,B,B,D,B,D,B,B,D,B,B,H,H,B,D,B,B,H,H,B,B,D,D,D,D,D,B,B,H,B,B,B,B,D,B,D,B,D,D,D,D,D,H,B,D,D,B,D,B,B,D,D,B,D,D,B,B,B,B,B,B,B,D
rs6365999,B,B,D,D,D,B,B,D,B,B,D,D,B,D,D,D,D,B,B,B,D,B,D,D,B,B,B,B,B,B,B,B,B,D,B,D,B,B,D,B,B,H,H,B,D,B,B,H,H,B,B,D,D,D,D,D,B,B,H,B,B,B,B,D,B,D,B,D,D,D,D,D,H,B,D,D,B,D,B,B,D,D,B,D,D,B,B,B,B,B,B,U,D
rs6376963,B,B,D,D,D,B,B,D,B,B,D,D,B,D,D,D,D,B,B,B,D,B,D,D,B,B,B,B,B,B,B,B,B,D,B,D,B,D,D,B,B,H,H,B,B,B,B,H,H,B,B,D,D,D,D,B,B,B,H,B,B,B,B,D,B,D,B,D,D,D,D,D,H,B,D,D,B,D,B,B,D,D,B,D,D,B,B,B,B,B,B,U,D
The meta file can be fetched with
curl "http://test-gn2.genenetwork.org/api_pre1/genotype/mouse/BXD.json"returning
{"description":"BXD","crosstype":"riself","geno":"BXD/BXD_geno_6a766888cf7a5b5b9376ee165b4518ab_20150722.csv","geno_transposed":true,"metadata":{"original":{"source":"GeneNetwork","unique_id":"42171462281377824604ec3d83771d79","date":"20150722"},"geno":{"unique_id":"6a766888cf7a5b5b9376ee165b4518ab","date":"20150722"},"gmap":{"unique_id":"dfbafbe862fb3572d3e847b7b7859540","date":"20150722"},"genotypes_descr":{"1":"maternal","2":"paternal","3":"heterozygous"}},"genotypes":{"B":1,"D":2,"H":3},"x_chr":"X","na.strings":["U"],"gmap":"BXD/BXD_gmap_dfbafbe862fb3572d3e847b7b7859540_20150722.csv"}which describes the genotypes used (B, D and U).
Furthermore there is the gmap data with
curl "http://test-gn2.genenetwork.org/api_pre1/genotype/mouse/BXD/gmap.csv"rs6269442,1,0.0,3.482275
rs6365999,1,0.0,4.811062
rs6376963,1,0.895,5.008089
A larger BXD17K is available through
curl "http://test-gn2.genenetwork.org/api_pre1/genotype/mouse/BXD17K.json"