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As you can see, the last two columns where the sequence and quality scores should usually be, are replaced with wildcards. Is this expected behavior? If so, why? Does this not present any problems for downstream analysis?
The text was updated successfully, but these errors were encountered:
These are not included because it is unclear what to report when you are shifting reads (if you drop --ATACshift, both fields would be retained). In case you have a specific use-case here, feel free to re-open and explain what the expected behavior should be with regards to the new coordinates after shift.
deepTools version: deeptools/3.5.0
python version: Python 2.7.12
Command:
alignmentSieve --numberOfProcessors max --ATACshift --blackListFileName ${blacklist}/hg38-blacklist.v2.bed --bam ${sample}.blacklist-filtered.bam -o ${sample}-shifted.bam
head of output bam file:
As you can see, the last two columns where the sequence and quality scores should usually be, are replaced with wildcards. Is this expected behavior? If so, why? Does this not present any problems for downstream analysis?
The text was updated successfully, but these errors were encountered: