This workflow supplements the Galaxy-P tutorial on database handling (Link). You will find two versions of the same workflow: one creates a database with only the cRAP proteins added, the other further adds the proteomes of the most common mycoplasma contaminants.

An overview of the workflow is given below:

For this workflow, you need no prior inputs. You can change the database to download after starting to run the workflow (default: Homo sapiens). To do so, click on the Taxonomy option in 1: Protein Database Downloader. You may also change other options about the database to download (reviewed, Proteome Set and Include isoform data). Please refer to the database handling tutorial for details.

The workflow provides two outputs:

1. The database with cRAP (and mycoplasma) added, and
2. The same database with a decoy database with reverse protein sequences attached.