You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
pe1_path: T51_S8_R1_001.fastq.gz
pe2_path: T51_S8_R2_001.fastq.gz
genome_path: assembly_k41.contigs.fa
output_path: assembly.lib_0
tmp_path: /home/sp1615/Desktop/T51_genome_assembly/BESST_tmp
bwa path: bwa
number of threads: 40
Remove temp SAM and BAM files: No
Use bwa aln and sampe instead of bwa mem: No
Do not rebuild bwa index if already exists in tmp dir: No
Start processing.
Aligning with bwa mem.
Temp directory: /home/sp1615/Desktop/T51_genome_assembly/BESST_tmp
Output path: assembly.lib_0
Stderr file: assembly.lib_0.bwa.1
Make bwa index... Done.
Align with bwa mem... Done.
Time elapsed for bwa index and mem: 5:43:13.571824
Convert SAM to BAM... Done.
Time elapsed for SAM to BAM conversion: 2:09:30.058622
Sort BAM... Done.
Time elapsed for BAM sorting: 4:17:52.790747
Index BAM... Done.
Time elapsed for BAM indexing: 0:14:52.160132
Remove temp files... Done.
Time elapsed for temp files removing: 0:00:16.807376
I noticed here #25 that there seems to be an issue with how the gatb script calls BESST which can be fixed by changing line 469 from -orientation to --orientation.
however, this is the gatb script version I have at line 469:
hi, yes please try this change, but also if you have only paired-end reads, you could also just skip scaffolding or pre-merge pairs if they're overlapping. Assembly result shouldn't be drastically different.
Thank you for your reply, I have tried changing it to -orientation and it works.
also, I tried multi-k assembly + scaffolding (I managed to increase my storage) and it all works, but I have yet to check whether the scaffolding with BESST has improved the asssemblies at various k values.
Hello!
I am trying to use GATB-minia-pipeline to assemble a genome. Because my storage is limited, I am not doing a multi-k but instead a k=41.
the assembly with minia works without issues, however when performing a scaffold with BESST I ran into the following issue:
(2021-08-10 11:41:36) Finished Multi-k GATB-Pipeline at k=41
(2021-08-10 11:41:38) Execution of 'python BESST/scripts/reads_to_ctg_map.py'. Command line:
python /home/sp1615/gatb-minia-pipeline/BESST/scripts/reads_to_ctg_map.py --tmp_path /home/sp1615/Desktop/T51_genome_assembly/BESST_tmp --threads 40 T51_S8_R1_001.fastq.gz T51_S8_R2_001.fastq.gz assembly_k41.contigs.fa assembly.lib_0
pe1_path: T51_S8_R1_001.fastq.gz
pe2_path: T51_S8_R2_001.fastq.gz
genome_path: assembly_k41.contigs.fa
output_path: assembly.lib_0
tmp_path: /home/sp1615/Desktop/T51_genome_assembly/BESST_tmp
bwa path: bwa
number of threads: 40
Remove temp SAM and BAM files: No
Use bwa aln and sampe instead of bwa mem: No
Do not rebuild bwa index if already exists in tmp dir: No
Start processing.
Aligning with bwa mem.
Temp directory: /home/sp1615/Desktop/T51_genome_assembly/BESST_tmp
Output path: assembly.lib_0
Stderr file: assembly.lib_0.bwa.1
Make bwa index... Done.
Align with bwa mem... Done.
Time elapsed for bwa index and mem: 5:43:13.571824
Convert SAM to BAM... Done.
Time elapsed for SAM to BAM conversion: 2:09:30.058622
Sort BAM... Done.
Time elapsed for BAM sorting: 4:17:52.790747
Index BAM... Done.
Time elapsed for BAM indexing: 0:14:52.160132
Remove temp files... Done.
Time elapsed for temp files removing: 0:00:16.807376
Processing is finished.
(2021-08-11 00:07:30) Execution of 'python BESST/runBESST'. Command line:
/home/sp1615/gatb-minia-pipeline/tools/memused python /home/sp1615/gatb-minia-pipeline/BESST/runBESST -c assembly_k41.contigs.fa -f assembly.lib_0.bam -o assembly_besst --orientation fr --iter 10000
usage: BESST [-h] -c CONTIGFILE -f BAMFILES [BAMFILES ...] -orientation
ORIENTATION [ORIENTATION ...] [-r READLEN [READLEN ...]]
[-m MEAN [MEAN ...]] [-s STDDEV [STDDEV ...]] [-z COVCUTOFF]
[-z_min LOWER_COVCUTOFF] [-T THRESHOLD [THRESHOLD ...]]
[-e EDGESUPPORT [EDGESUPPORT ...]] [-k MINSIZE [MINSIZE ...]]
[-filter_contigs CONTIG_FILTER_LENGTH] [--min_mapq MIN_MAPQ]
[--iter PATH_THRESHOLD] [--score_cutoff SCORE_CUTOFF]
[--max_extensions MAX_EXTENSIONS] [-a HAPLRATIO]
[-b HAPLTHRESHOLD] [-K KMER] [-M MMER] [-g] [-o OUTPUT] [-d] [-y]
[-q] [--no_score] [-devel] [-plots] [--separate_repeats]
[--NO_ILP] [--FASTER_ILP] [--print_scores] [--dfs_traversal]
[--bfs_traversal] [-max_contig_overlap MAX_CONTIG_OVERLAP]
[--version]
BESST: error: argument -orientation is required
maximal memory used: 6 MB
(2021-08-11 00:07:31) Execution of 'python BESST/runBESST' failed. Command line:
/home/sp1615/gatb-minia-pipeline/tools/memused python /home/sp1615/gatb-minia-pipeline/BESST/runBESST -c assembly_k41.contigs.fa -f assembly.lib_0.bam -o assembly_besst --orientation fr --iter 10000
I noticed here #25 that there seems to be an issue with how the gatb script calls BESST which can be fixed by changing line 469 from -orientation to --orientation.
however, this is the gatb script version I have at line 469:
cmd = ['-c', contigs, '-f'] + bam_files + ['-o', prefix + '_besst'] + ['--orientation'] + orientations + ['--iter', besst_iter]
which already has the change from -orientation to --orientation.
should I change it back to -orientation?
thank you for your help!
S
The text was updated successfully, but these errors were encountered: