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APDNS(Automatic pathogen diagnosis by nanopore sequencing)

  • Usage:
    APDNS.sh -n barcode01 -i /input/ -s /APDNS/ -b SQK-PBK004
    *-n is the barcode ID.
    *-i is the input files. (.fastq.gz)
    *-s is the scripts pathway.
    *-b is the library preparation kit ID.
  • Results description:
    *barcode01_nohuman.fastq: All reads of nonhuman species.
    *barcode01.output: Containing two columns of data, the first column is reads number of each species, the second column is species names.
    *barcode01.statistic.csv: Containing four columns (species name, read_num, RPM, % of microbe).
    read_num means reads number of each species.
    RPM means proportion of species reads in the total reads.
    % of microbe means proportion of species reads in the microbial reads.

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