/
readalignment.pb.go
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/
readalignment.pb.go
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// Copyright 2016 Google Inc.
//
// Licensed under the Apache License, Version 2.0 (the "License");
// you may not use this file except in compliance with the License.
// You may obtain a copy of the License at
//
// http://www.apache.org/licenses/LICENSE-2.0
//
// Unless required by applicable law or agreed to in writing, software
// distributed under the License is distributed on an "AS IS" BASIS,
// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
// See the License for the specific language governing permissions and
// limitations under the License.
// Code generated by protoc-gen-go. DO NOT EDIT.
// versions:
// protoc-gen-go v1.26.0
// protoc v3.12.2
// source: google/genomics/v1/readalignment.proto
package genomics
import (
reflect "reflect"
sync "sync"
_ "google.golang.org/genproto/googleapis/api/annotations"
protoreflect "google.golang.org/protobuf/reflect/protoreflect"
protoimpl "google.golang.org/protobuf/runtime/protoimpl"
structpb "google.golang.org/protobuf/types/known/structpb"
)
const (
// Verify that this generated code is sufficiently up-to-date.
_ = protoimpl.EnforceVersion(20 - protoimpl.MinVersion)
// Verify that runtime/protoimpl is sufficiently up-to-date.
_ = protoimpl.EnforceVersion(protoimpl.MaxVersion - 20)
)
// A linear alignment can be represented by one CIGAR string. Describes the
// mapped position and local alignment of the read to the reference.
type LinearAlignment struct {
state protoimpl.MessageState
sizeCache protoimpl.SizeCache
unknownFields protoimpl.UnknownFields
// The position of this alignment.
Position *Position `protobuf:"bytes,1,opt,name=position,proto3" json:"position,omitempty"`
// The mapping quality of this alignment. Represents how likely
// the read maps to this position as opposed to other locations.
//
// Specifically, this is -10 log10 Pr(mapping position is wrong), rounded to
// the nearest integer.
MappingQuality int32 `protobuf:"varint,2,opt,name=mapping_quality,json=mappingQuality,proto3" json:"mapping_quality,omitempty"`
// Represents the local alignment of this sequence (alignment matches, indels,
// etc) against the reference.
Cigar []*CigarUnit `protobuf:"bytes,3,rep,name=cigar,proto3" json:"cigar,omitempty"`
}
func (x *LinearAlignment) Reset() {
*x = LinearAlignment{}
if protoimpl.UnsafeEnabled {
mi := &file_google_genomics_v1_readalignment_proto_msgTypes[0]
ms := protoimpl.X.MessageStateOf(protoimpl.Pointer(x))
ms.StoreMessageInfo(mi)
}
}
func (x *LinearAlignment) String() string {
return protoimpl.X.MessageStringOf(x)
}
func (*LinearAlignment) ProtoMessage() {}
func (x *LinearAlignment) ProtoReflect() protoreflect.Message {
mi := &file_google_genomics_v1_readalignment_proto_msgTypes[0]
if protoimpl.UnsafeEnabled && x != nil {
ms := protoimpl.X.MessageStateOf(protoimpl.Pointer(x))
if ms.LoadMessageInfo() == nil {
ms.StoreMessageInfo(mi)
}
return ms
}
return mi.MessageOf(x)
}
// Deprecated: Use LinearAlignment.ProtoReflect.Descriptor instead.
func (*LinearAlignment) Descriptor() ([]byte, []int) {
return file_google_genomics_v1_readalignment_proto_rawDescGZIP(), []int{0}
}
func (x *LinearAlignment) GetPosition() *Position {
if x != nil {
return x.Position
}
return nil
}
func (x *LinearAlignment) GetMappingQuality() int32 {
if x != nil {
return x.MappingQuality
}
return 0
}
func (x *LinearAlignment) GetCigar() []*CigarUnit {
if x != nil {
return x.Cigar
}
return nil
}
// A read alignment describes a linear alignment of a string of DNA to a
// [reference sequence][google.genomics.v1.Reference], in addition to metadata
// about the fragment (the molecule of DNA sequenced) and the read (the bases
// which were read by the sequencer). A read is equivalent to a line in a SAM
// file. A read belongs to exactly one read group and exactly one
// [read group set][google.genomics.v1.ReadGroupSet].
//
// For more genomics resource definitions, see [Fundamentals of Google
// Genomics](https://cloud.google.com/genomics/fundamentals-of-google-genomics)
//
// ### Reverse-stranded reads
//
// Mapped reads (reads having a non-null `alignment`) can be aligned to either
// the forward or the reverse strand of their associated reference. Strandedness
// of a mapped read is encoded by `alignment.position.reverseStrand`.
//
// If we consider the reference to be a forward-stranded coordinate space of
// `[0, reference.length)` with `0` as the left-most position and
// `reference.length` as the right-most position, reads are always aligned left
// to right. That is, `alignment.position.position` always refers to the
// left-most reference coordinate and `alignment.cigar` describes the alignment
// of this read to the reference from left to right. All per-base fields such as
// `alignedSequence` and `alignedQuality` share this same left-to-right
// orientation; this is true of reads which are aligned to either strand. For
// reverse-stranded reads, this means that `alignedSequence` is the reverse
// complement of the bases that were originally reported by the sequencing
// machine.
//
// ### Generating a reference-aligned sequence string
//
// When interacting with mapped reads, it's often useful to produce a string
// representing the local alignment of the read to reference. The following
// pseudocode demonstrates one way of doing this:
//
// out = ""
// offset = 0
// for c in read.alignment.cigar {
// switch c.operation {
// case "ALIGNMENT_MATCH", "SEQUENCE_MATCH", "SEQUENCE_MISMATCH":
// out += read.alignedSequence[offset:offset+c.operationLength]
// offset += c.operationLength
// break
// case "CLIP_SOFT", "INSERT":
// offset += c.operationLength
// break
// case "PAD":
// out += repeat("*", c.operationLength)
// break
// case "DELETE":
// out += repeat("-", c.operationLength)
// break
// case "SKIP":
// out += repeat(" ", c.operationLength)
// break
// case "CLIP_HARD":
// break
// }
// }
// return out
//
// ### Converting to SAM's CIGAR string
//
// The following pseudocode generates a SAM CIGAR string from the
// `cigar` field. Note that this is a lossy conversion
// (`cigar.referenceSequence` is lost).
//
// cigarMap = {
// "ALIGNMENT_MATCH": "M",
// "INSERT": "I",
// "DELETE": "D",
// "SKIP": "N",
// "CLIP_SOFT": "S",
// "CLIP_HARD": "H",
// "PAD": "P",
// "SEQUENCE_MATCH": "=",
// "SEQUENCE_MISMATCH": "X",
// }
// cigarStr = ""
// for c in read.alignment.cigar {
// cigarStr += c.operationLength + cigarMap[c.operation]
// }
// return cigarStr
type Read struct {
state protoimpl.MessageState
sizeCache protoimpl.SizeCache
unknownFields protoimpl.UnknownFields
// The server-generated read ID, unique across all reads. This is different
// from the `fragmentName`.
Id string `protobuf:"bytes,1,opt,name=id,proto3" json:"id,omitempty"`
// The ID of the read group this read belongs to. A read belongs to exactly
// one read group. This is a server-generated ID which is distinct from SAM's
// RG tag (for that value, see
// [ReadGroup.name][google.genomics.v1.ReadGroup.name]).
ReadGroupId string `protobuf:"bytes,2,opt,name=read_group_id,json=readGroupId,proto3" json:"read_group_id,omitempty"`
// The ID of the read group set this read belongs to. A read belongs to
// exactly one read group set.
ReadGroupSetId string `protobuf:"bytes,3,opt,name=read_group_set_id,json=readGroupSetId,proto3" json:"read_group_set_id,omitempty"`
// The fragment name. Equivalent to QNAME (query template name) in SAM.
FragmentName string `protobuf:"bytes,4,opt,name=fragment_name,json=fragmentName,proto3" json:"fragment_name,omitempty"`
// The orientation and the distance between reads from the fragment are
// consistent with the sequencing protocol (SAM flag 0x2).
ProperPlacement bool `protobuf:"varint,5,opt,name=proper_placement,json=properPlacement,proto3" json:"proper_placement,omitempty"`
// The fragment is a PCR or optical duplicate (SAM flag 0x400).
DuplicateFragment bool `protobuf:"varint,6,opt,name=duplicate_fragment,json=duplicateFragment,proto3" json:"duplicate_fragment,omitempty"`
// The observed length of the fragment, equivalent to TLEN in SAM.
FragmentLength int32 `protobuf:"varint,7,opt,name=fragment_length,json=fragmentLength,proto3" json:"fragment_length,omitempty"`
// The read number in sequencing. 0-based and less than numberReads. This
// field replaces SAM flag 0x40 and 0x80.
ReadNumber int32 `protobuf:"varint,8,opt,name=read_number,json=readNumber,proto3" json:"read_number,omitempty"`
// The number of reads in the fragment (extension to SAM flag 0x1).
NumberReads int32 `protobuf:"varint,9,opt,name=number_reads,json=numberReads,proto3" json:"number_reads,omitempty"`
// Whether this read did not pass filters, such as platform or vendor quality
// controls (SAM flag 0x200).
FailedVendorQualityChecks bool `protobuf:"varint,10,opt,name=failed_vendor_quality_checks,json=failedVendorQualityChecks,proto3" json:"failed_vendor_quality_checks,omitempty"`
// The linear alignment for this alignment record. This field is null for
// unmapped reads.
Alignment *LinearAlignment `protobuf:"bytes,11,opt,name=alignment,proto3" json:"alignment,omitempty"`
// Whether this alignment is secondary. Equivalent to SAM flag 0x100.
// A secondary alignment represents an alternative to the primary alignment
// for this read. Aligners may return secondary alignments if a read can map
// ambiguously to multiple coordinates in the genome. By convention, each read
// has one and only one alignment where both `secondaryAlignment`
// and `supplementaryAlignment` are false.
SecondaryAlignment bool `protobuf:"varint,12,opt,name=secondary_alignment,json=secondaryAlignment,proto3" json:"secondary_alignment,omitempty"`
// Whether this alignment is supplementary. Equivalent to SAM flag 0x800.
// Supplementary alignments are used in the representation of a chimeric
// alignment. In a chimeric alignment, a read is split into multiple
// linear alignments that map to different reference contigs. The first
// linear alignment in the read will be designated as the representative
// alignment; the remaining linear alignments will be designated as
// supplementary alignments. These alignments may have different mapping
// quality scores. In each linear alignment in a chimeric alignment, the read
// will be hard clipped. The `alignedSequence` and
// `alignedQuality` fields in the alignment record will only
// represent the bases for its respective linear alignment.
SupplementaryAlignment bool `protobuf:"varint,13,opt,name=supplementary_alignment,json=supplementaryAlignment,proto3" json:"supplementary_alignment,omitempty"`
// The bases of the read sequence contained in this alignment record,
// **without CIGAR operations applied** (equivalent to SEQ in SAM).
// `alignedSequence` and `alignedQuality` may be
// shorter than the full read sequence and quality. This will occur if the
// alignment is part of a chimeric alignment, or if the read was trimmed. When
// this occurs, the CIGAR for this read will begin/end with a hard clip
// operator that will indicate the length of the excised sequence.
AlignedSequence string `protobuf:"bytes,14,opt,name=aligned_sequence,json=alignedSequence,proto3" json:"aligned_sequence,omitempty"`
// The quality of the read sequence contained in this alignment record
// (equivalent to QUAL in SAM).
// `alignedSequence` and `alignedQuality` may be shorter than the full read
// sequence and quality. This will occur if the alignment is part of a
// chimeric alignment, or if the read was trimmed. When this occurs, the CIGAR
// for this read will begin/end with a hard clip operator that will indicate
// the length of the excised sequence.
AlignedQuality []int32 `protobuf:"varint,15,rep,packed,name=aligned_quality,json=alignedQuality,proto3" json:"aligned_quality,omitempty"`
// The mapping of the primary alignment of the
// `(readNumber+1)%numberReads` read in the fragment. It replaces
// mate position and mate strand in SAM.
NextMatePosition *Position `protobuf:"bytes,16,opt,name=next_mate_position,json=nextMatePosition,proto3" json:"next_mate_position,omitempty"`
// A map of additional read alignment information. This must be of the form
// map<string, string[]> (string key mapping to a list of string values).
Info map[string]*structpb.ListValue `protobuf:"bytes,17,rep,name=info,proto3" json:"info,omitempty" protobuf_key:"bytes,1,opt,name=key,proto3" protobuf_val:"bytes,2,opt,name=value,proto3"`
}
func (x *Read) Reset() {
*x = Read{}
if protoimpl.UnsafeEnabled {
mi := &file_google_genomics_v1_readalignment_proto_msgTypes[1]
ms := protoimpl.X.MessageStateOf(protoimpl.Pointer(x))
ms.StoreMessageInfo(mi)
}
}
func (x *Read) String() string {
return protoimpl.X.MessageStringOf(x)
}
func (*Read) ProtoMessage() {}
func (x *Read) ProtoReflect() protoreflect.Message {
mi := &file_google_genomics_v1_readalignment_proto_msgTypes[1]
if protoimpl.UnsafeEnabled && x != nil {
ms := protoimpl.X.MessageStateOf(protoimpl.Pointer(x))
if ms.LoadMessageInfo() == nil {
ms.StoreMessageInfo(mi)
}
return ms
}
return mi.MessageOf(x)
}
// Deprecated: Use Read.ProtoReflect.Descriptor instead.
func (*Read) Descriptor() ([]byte, []int) {
return file_google_genomics_v1_readalignment_proto_rawDescGZIP(), []int{1}
}
func (x *Read) GetId() string {
if x != nil {
return x.Id
}
return ""
}
func (x *Read) GetReadGroupId() string {
if x != nil {
return x.ReadGroupId
}
return ""
}
func (x *Read) GetReadGroupSetId() string {
if x != nil {
return x.ReadGroupSetId
}
return ""
}
func (x *Read) GetFragmentName() string {
if x != nil {
return x.FragmentName
}
return ""
}
func (x *Read) GetProperPlacement() bool {
if x != nil {
return x.ProperPlacement
}
return false
}
func (x *Read) GetDuplicateFragment() bool {
if x != nil {
return x.DuplicateFragment
}
return false
}
func (x *Read) GetFragmentLength() int32 {
if x != nil {
return x.FragmentLength
}
return 0
}
func (x *Read) GetReadNumber() int32 {
if x != nil {
return x.ReadNumber
}
return 0
}
func (x *Read) GetNumberReads() int32 {
if x != nil {
return x.NumberReads
}
return 0
}
func (x *Read) GetFailedVendorQualityChecks() bool {
if x != nil {
return x.FailedVendorQualityChecks
}
return false
}
func (x *Read) GetAlignment() *LinearAlignment {
if x != nil {
return x.Alignment
}
return nil
}
func (x *Read) GetSecondaryAlignment() bool {
if x != nil {
return x.SecondaryAlignment
}
return false
}
func (x *Read) GetSupplementaryAlignment() bool {
if x != nil {
return x.SupplementaryAlignment
}
return false
}
func (x *Read) GetAlignedSequence() string {
if x != nil {
return x.AlignedSequence
}
return ""
}
func (x *Read) GetAlignedQuality() []int32 {
if x != nil {
return x.AlignedQuality
}
return nil
}
func (x *Read) GetNextMatePosition() *Position {
if x != nil {
return x.NextMatePosition
}
return nil
}
func (x *Read) GetInfo() map[string]*structpb.ListValue {
if x != nil {
return x.Info
}
return nil
}
var File_google_genomics_v1_readalignment_proto protoreflect.FileDescriptor
var file_google_genomics_v1_readalignment_proto_rawDesc = []byte{
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}
var (
file_google_genomics_v1_readalignment_proto_rawDescOnce sync.Once
file_google_genomics_v1_readalignment_proto_rawDescData = file_google_genomics_v1_readalignment_proto_rawDesc
)
func file_google_genomics_v1_readalignment_proto_rawDescGZIP() []byte {
file_google_genomics_v1_readalignment_proto_rawDescOnce.Do(func() {
file_google_genomics_v1_readalignment_proto_rawDescData = protoimpl.X.CompressGZIP(file_google_genomics_v1_readalignment_proto_rawDescData)
})
return file_google_genomics_v1_readalignment_proto_rawDescData
}
var file_google_genomics_v1_readalignment_proto_msgTypes = make([]protoimpl.MessageInfo, 3)
var file_google_genomics_v1_readalignment_proto_goTypes = []interface{}{
(*LinearAlignment)(nil), // 0: google.genomics.v1.LinearAlignment
(*Read)(nil), // 1: google.genomics.v1.Read
nil, // 2: google.genomics.v1.Read.InfoEntry
(*Position)(nil), // 3: google.genomics.v1.Position
(*CigarUnit)(nil), // 4: google.genomics.v1.CigarUnit
(*structpb.ListValue)(nil), // 5: google.protobuf.ListValue
}
var file_google_genomics_v1_readalignment_proto_depIdxs = []int32{
3, // 0: google.genomics.v1.LinearAlignment.position:type_name -> google.genomics.v1.Position
4, // 1: google.genomics.v1.LinearAlignment.cigar:type_name -> google.genomics.v1.CigarUnit
0, // 2: google.genomics.v1.Read.alignment:type_name -> google.genomics.v1.LinearAlignment
3, // 3: google.genomics.v1.Read.next_mate_position:type_name -> google.genomics.v1.Position
2, // 4: google.genomics.v1.Read.info:type_name -> google.genomics.v1.Read.InfoEntry
5, // 5: google.genomics.v1.Read.InfoEntry.value:type_name -> google.protobuf.ListValue
6, // [6:6] is the sub-list for method output_type
6, // [6:6] is the sub-list for method input_type
6, // [6:6] is the sub-list for extension type_name
6, // [6:6] is the sub-list for extension extendee
0, // [0:6] is the sub-list for field type_name
}
func init() { file_google_genomics_v1_readalignment_proto_init() }
func file_google_genomics_v1_readalignment_proto_init() {
if File_google_genomics_v1_readalignment_proto != nil {
return
}
file_google_genomics_v1_cigar_proto_init()
file_google_genomics_v1_position_proto_init()
if !protoimpl.UnsafeEnabled {
file_google_genomics_v1_readalignment_proto_msgTypes[0].Exporter = func(v interface{}, i int) interface{} {
switch v := v.(*LinearAlignment); i {
case 0:
return &v.state
case 1:
return &v.sizeCache
case 2:
return &v.unknownFields
default:
return nil
}
}
file_google_genomics_v1_readalignment_proto_msgTypes[1].Exporter = func(v interface{}, i int) interface{} {
switch v := v.(*Read); i {
case 0:
return &v.state
case 1:
return &v.sizeCache
case 2:
return &v.unknownFields
default:
return nil
}
}
}
type x struct{}
out := protoimpl.TypeBuilder{
File: protoimpl.DescBuilder{
GoPackagePath: reflect.TypeOf(x{}).PkgPath(),
RawDescriptor: file_google_genomics_v1_readalignment_proto_rawDesc,
NumEnums: 0,
NumMessages: 3,
NumExtensions: 0,
NumServices: 0,
},
GoTypes: file_google_genomics_v1_readalignment_proto_goTypes,
DependencyIndexes: file_google_genomics_v1_readalignment_proto_depIdxs,
MessageInfos: file_google_genomics_v1_readalignment_proto_msgTypes,
}.Build()
File_google_genomics_v1_readalignment_proto = out.File
file_google_genomics_v1_readalignment_proto_rawDesc = nil
file_google_genomics_v1_readalignment_proto_goTypes = nil
file_google_genomics_v1_readalignment_proto_depIdxs = nil
}