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Problem running scde on linux cluster. Fortran "dqrls" not resolved #55
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Hi Florian, Thanks for the thorough documentation of your issue. Can you please try to see if the error persists with Also, can you please check if the multicore issue is being caused by mclapply or bplapply? We currently handle multicore processing using this function:
You can explicitly modify the
or just add a |
I tried running knn.error.models and it seems to calculate error models correctly when using
When setting linear fit to false, it will still give the same error.
Using the papply code right in front of calculating error models seem to fix the multicore issue still gives me this error after execution for the code for 1 comparison group in my data. (I am iterating over clusters from my single cells and doing DE for two groups in each cluster).
|
Sorry, so the multicore module still fails for me at this point. I think I am using it wrong. How exactly do I have to implement the papply solution? I actually need the multicore since single core is really slow when comparing hundreds of cells. Any other tips for speed improvement? |
So I have been trying around with this a bit now and have not found a solution. Single core on my cells takes way too long. The error seems to come from bplapply for me but none of the modes in the papply function seems to work on our cluster. Any suggestions?! |
Can you please try a simple test:
This should take about 10 seconds.
This should take less than 10 seconds, though there's time associated with forking so it should be more than 1 second. If you want to benchmark the time spent for forking, you can try:
And the difference between t3 and t2 should be about 1 second if your mclapply is working properly. And lastly:
If these are failing on your cluster, then it is an issue with the parallelization. |
Just ran the tests and it seemed to execute all of them without any problems...
So seems its not a problem with these packages? Do I need to specify BiocParallel or parralel when running scde? I was guessing they are dependencies and are automatically loaded? |
They should be automatically loaded. Based on the error you saw previously:
it sounds like this could be a memory issue ("*** caught segfault *** address 0xf2d1, cause 'memory not mapped'" is a more common error message for really large datasets). Does the error reproduce for the example data provided with the package or just your own data? |
I might be having a similar problem as I am getting the following error when running scde.error.models:
I will try out the different apply functions and let you know |
Hi there,
I am trying to run SCDE on a Linux computational cluster.
I am getting the following error when running on a single core:
Which seems to be a similar issue to this thread:
(https://github.com/hms-dbmi/scde/issues/21)
I have recompiled SCDE from the binary of the developer version but this did not solve the issue. I also tried revertingg the flexmix version and using it with the stable scde version. I tried both of this on the cluster as well on my local machine and both gave the same error.
When trying with multiple cores, I am getting the same problem described here:
(https://github.com/hms-dbmi/scde/issues/31)
Any suggestions what I can try for either single core but preferably to get the multicore command do work?
My sessionInfo() :
R version 3.4.0 (2017-04-21)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Scientific Linux release 6.3 (Carbon)
Matrix products: default
BLAS: /home/apps/Logiciels/R/3.4.0-gcc/lib64/R/lib/libRblas.so
LAPACK: /home/apps/Logiciels/R/3.4.0-gcc/lib64/R/lib/libRlapack.so
locale:
[1] C
attached base packages:
[1] stats4 grDevices datasets parallel stats graphics utils
[8] methods base
other attached packages:
[1] scde_2.7.0 flexmix_2.3-13
[3] lattice_0.20-35 bindrcpp_0.2
[5] qvalue_2.10.0 edgeR_3.20.1
[7] limma_3.34.1 gtools_3.5.0
[9] scater_1.6.0 SingleCellExperiment_1.0.0
[11] SummarizedExperiment_1.8.0 DelayedArray_0.4.1
[13] matrixStats_0.52.2 GenomicRanges_1.30.0
[15] GenomeInfoDb_1.14.0 IRanges_2.12.0
[17] S4Vectors_0.16.0 ggrepel_0.7.0
[19] RColorBrewer_1.1-2 ggsci_2.8
[21] dplyr_0.7.4 tidyr_0.7.2
[23] data.table_1.10.4-3 Seurat_2.1.0
[25] Matrix_1.2-9 cowplot_0.8.0
[27] ggplot2_2.2.1 pander_0.6.1
[29] knitr_1.16 bigmemory_4.5.31
[31] bigmemory.sri_0.1.3 Biobase_2.38.0
[33] BiocGenerics_0.24.0
loaded via a namespace (and not attached):
[1] shinydashboard_0.6.1 R.utils_2.6.0
[3] lme4_1.1-13 RSQLite_2.0
[5] AnnotationDbi_1.40.0 htmlwidgets_0.9
[7] grid_3.4.0 trimcluster_0.1-2
[9] ranger_0.8.0 BiocParallel_1.12.0
[11] Rtsne_0.13 munsell_0.4.3
[13] codetools_0.2-15 ica_1.0-1
[15] colorspace_1.3-2 ROCR_1.0-7
[17] robustbase_0.92-7 dtw_1.18-1
[19] distillery_1.0-4 NMF_0.20.6
[21] labeling_0.3 lars_1.2
[23] tximport_1.6.0 GenomeInfoDbData_0.99.1
[25] mnormt_1.5-5 bit64_0.9-7
[27] extRemes_2.0-8 rhdf5_2.22.0
[29] diptest_0.75-7 R6_2.2.2
[31] doParallel_1.0.11 ggbeeswarm_0.6.0
[33] VGAM_1.0-4 locfit_1.5-9.1
[35] RcppArmadillo_0.8.100.1.0 bitops_1.0-6
[37] assertthat_0.2.0 SDMTools_1.1-221
[39] scales_0.4.1 nnet_7.3-12
[41] ggjoy_0.3.0 beeswarm_0.2.3
[43] gtable_0.2.0 Cairo_1.5-9
[45] rlang_0.1.4 MatrixModels_0.4-1
[47] scatterplot3d_0.3-40 splines_3.4.0
[49] lazyeval_0.2.0 ModelMetrics_1.1.0
[51] acepack_1.4.1 brew_1.0-6
[53] checkmate_1.8.3 reshape2_1.4.2
[55] backports_1.1.0 httpuv_1.3.5
[57] Hmisc_4.0-3 caret_6.0-76
[59] tools_3.4.0 gridBase_0.4-7
[61] gplots_3.0.1 proxy_0.4-17
[63] Rcpp_0.12.14 plyr_1.8.4
[65] base64enc_0.1-3 progress_1.1.2
[67] zlibbioc_1.24.0 purrr_0.2.4
[69] RCurl_1.95-4.8 prettyunits_1.0.2
[71] rpart_4.1-11 pbapply_1.3-3
[73] viridis_0.4.0 cluster_2.0.6
[75] magrittr_1.5 SparseM_1.77
[77] pcaMethods_1.70.0 mvtnorm_1.0-6
[79] mime_0.5 xtable_1.8-2
[81] pbkrtest_0.4-7 XML_3.98-1.9
[83] mclust_5.3 RMTstat_0.3
[85] gridExtra_2.2.1 compiler_3.4.0
[87] biomaRt_2.34.0 tibble_1.3.4
[89] KernSmooth_2.23-15 minqa_1.2.4
[91] R.oo_1.21.0 htmltools_0.3.6
[93] segmented_0.5-2.1 mgcv_1.8-17
[95] Formula_1.2-2 tclust_1.2-7
[97] DBI_0.7 diffusionMap_1.1-0
[99] MASS_7.3-47 fpc_2.1-10
[101] car_2.1-6 R.methodsS3_1.7.1
[103] gdata_2.18.0 bindr_0.1
[105] igraph_1.1.2 pkgconfig_2.0.1
[107] sn_1.5-0 registry_0.3
[109] numDeriv_2016.8-1 foreign_0.8-67
[111] foreach_1.4.3 vipor_0.4.5
[113] rngtools_1.2.4 pkgmaker_0.22
[115] XVector_0.18.0 stringr_1.2.0
[117] digest_0.6.12 tsne_0.1-3
[119] Rook_1.1-1 htmlTable_1.9
[121] kernlab_0.9-25 Lmoments_1.2-3
[123] shiny_1.0.3 quantreg_5.34
[125] modeltools_0.2-21 rjson_0.2.15
[127] nloptr_1.0.4 nlme_3.1-131
[129] viridisLite_0.2.0 DEoptimR_1.0-8
[131] survival_2.41-3 glue_1.1.1
[133] FNN_1.1 prabclus_2.2-6
[135] iterators_1.0.8 bit_1.1-12
[137] class_7.3-14 stringi_1.1.5
[139] mixtools_1.1.0 blob_1.1.0
[141] latticeExtra_0.6-28 caTools_1.17.1
[143] memoise_1.1.0 irlba_2.2.1
[145] ape_4.1
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