the errors about the brie-count

[liuyu988]

i have .fa, .gtf, .bam, barcodes.tsv files. And i have run thebriekit-event and briekit-event-filter to gain the SE.filter.gtf.
now i run the brie-count, i can't unterstand the errors, would you give me a hand?

brie-count -a AS_events/SE.lenient.gtf -s cellsorted_possorted_genome.bam -b barcode.tsv.gz -o test -p 15

thanks!

[liuyu988]

errors:
Exception ignored in: 'pysam.libcalignmentfile.AlignmentFile.dealloc'
FileNotFoundError: [Errno 2] No such file or directory
[E::bgzf_read_block] Invalid BGZF header at offset 19866824255
[E::bgzf_read] Read block operation failed with error 2 after 0 of 4 bytes

[huangyh09]

not very sure what the issue is but seems incompatibility in the .gz file from the error message. Possibly, you can turn your .gz file into plain text or gzip it instead of bgzip.

Yuanhua