/
ThresholdAdjuster.java
960 lines (896 loc) · 28.9 KB
/
ThresholdAdjuster.java
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
package ij.plugin.frame;
import java.awt.*;
import java.awt.event.*;
import java.awt.image.*;
import ij.*;
import ij.plugin.*;
import ij.process.*;
import ij.gui.*;
import ij.measure.*;
import ij.plugin.frame.Recorder;
import ij.plugin.filter.*;
import ij.plugin.ChannelSplitter;
import ij.plugin.Thresholder;
/** Adjusts the lower and upper threshold levels of the active image. This
class is multi-threaded to provide a more responsive user interface. */
public class ThresholdAdjuster extends PlugInDialog implements PlugIn, Measurements,
Runnable, ActionListener, AdjustmentListener, ItemListener, ImageListener {
public static final String LOC_KEY = "threshold.loc";
public static final String MODE_KEY = "threshold.mode";
public static final String DARK_BACKGROUND = "threshold.dark";
static final int RED=0, BLACK_AND_WHITE=1, OVER_UNDER=2;
static final String[] modes = {"Red","B&W", "Over/Under"};
static final double defaultMinThreshold = 85;
static final double defaultMaxThreshold = 170;
static final int DEFAULT = 0;
static boolean fill1 = true;
static boolean fill2 = true;
static boolean useBW = true;
static boolean backgroundToNaN = true;
static ThresholdAdjuster instance;
static int mode = RED;
static String[] methodNames = AutoThresholder.getMethods();
static String method = methodNames[DEFAULT];
static AutoThresholder thresholder = new AutoThresholder();
ThresholdPlot plot = new ThresholdPlot();
Thread thread;
int minValue = -1;
int maxValue = -1;
int sliderRange = 256;
boolean doAutoAdjust,doReset,doApplyLut,doStateChange,doSet;
Panel panel;
Button autoB, resetB, applyB, setB;
int previousImageID;
int previousImageType;
int previousRoiHashCode;
double previousMin, previousMax;
int previousSlice;
boolean imageWasUpdated;
ImageJ ij;
double minThreshold, maxThreshold; // 0-255
Scrollbar minSlider, maxSlider;
Label label1, label2; // for current threshold
Label percentiles;
boolean done;
int lutColor;
Choice methodChoice, modeChoice;
Checkbox darkBackground, stackHistogram;
boolean firstActivation = true;
public ThresholdAdjuster() {
super("Threshold");
ImagePlus cimp = WindowManager.getCurrentImage();
if (cimp!=null && cimp.getBitDepth()==24) {
IJ.run(cimp, "Color Threshold...", "");
return;
}
if (instance!=null) {
instance.firstActivation = true;
instance.toFront();
instance.setup(cimp, true);
return;
}
WindowManager.addWindow(this);
instance = this;
mode = (int)Prefs.get(MODE_KEY, RED);
if (mode<RED || mode>OVER_UNDER) mode = RED;
setLutColor(mode);
IJ.register(PasteController.class);
ij = IJ.getInstance();
Font font = new Font("SansSerif", Font.PLAIN, 10);
GridBagLayout gridbag = new GridBagLayout();
GridBagConstraints c = new GridBagConstraints();
setLayout(gridbag);
// plot
int y = 0;
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.fill = GridBagConstraints.BOTH;
c.anchor = GridBagConstraints.CENTER;
c.insets = new Insets(10, 10, 0, 10); //top left bottom right
add(plot, c);
plot.addKeyListener(ij);
// percentiles
c.gridx = 0;
c.gridy = y++;
c.insets = new Insets(1, 10, 0, 10);
percentiles = new Label("");
percentiles.setFont(font);
add(percentiles, c);
// minThreshold slider
minSlider = new Scrollbar(Scrollbar.HORIZONTAL, sliderRange/3, 1, 0, sliderRange);
GUI.fix(minSlider);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh()?90:100;
c.fill = GridBagConstraints.HORIZONTAL;
c.insets = new Insets(1, 10, 0, 0);
add(minSlider, c);
minSlider.addAdjustmentListener(this);
minSlider.addKeyListener(ij);
minSlider.setUnitIncrement(1);
minSlider.setFocusable(false);
// minThreshold slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh()?10:0;
c.insets = new Insets(5, 0, 0, 10);
String text = IJ.isMacOSX()?"000000":"00000000";
label1 = new Label(text, Label.RIGHT);
label1.setFont(font);
add(label1, c);
// maxThreshold slider
maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, sliderRange*2/3, 1, 0, sliderRange);
GUI.fix(maxSlider);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = 100;
c.insets = new Insets(2, 10, 0, 0);
add(maxSlider, c);
maxSlider.addAdjustmentListener(this);
maxSlider.addKeyListener(ij);
maxSlider.setUnitIncrement(1);
maxSlider.setFocusable(false);
// maxThreshold slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(2, 0, 0, 10);
label2 = new Label(text, Label.RIGHT);
label2.setFont(font);
add(label2, c);
// choices
panel = new Panel();
methodChoice = new Choice();
for (int i=0; i<methodNames.length; i++)
methodChoice.addItem(methodNames[i]);
methodChoice.select(method);
methodChoice.addItemListener(this);
//methodChoice.addKeyListener(ij);
panel.add(methodChoice);
modeChoice = new Choice();
for (int i=0; i<modes.length; i++)
modeChoice.addItem(modes[i]);
modeChoice.select(mode);
modeChoice.addItemListener(this);
//modeChoice.addKeyListener(ij);
panel.add(modeChoice);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(8, 5, 0, 5);
c.anchor = GridBagConstraints.CENTER;
c.fill = GridBagConstraints.NONE;
add(panel, c);
// checkboxes
panel = new Panel();
boolean db = Prefs.get(DARK_BACKGROUND, Prefs.blackBackground?true:false);
darkBackground = new Checkbox("Dark background");
darkBackground.setState(db);
darkBackground.addItemListener(this);
panel.add(darkBackground);
stackHistogram = new Checkbox("Stack histogram");
stackHistogram.setState(false);
stackHistogram.addItemListener(this);
panel.add(stackHistogram);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(5, 5, 0, 5);
add(panel, c);
// buttons
int trim = IJ.isMacOSX()?11:0;
panel = new Panel();
autoB = new TrimmedButton("Auto",trim);
autoB.addActionListener(this);
autoB.addKeyListener(ij);
panel.add(autoB);
applyB = new TrimmedButton("Apply",trim);
applyB.addActionListener(this);
applyB.addKeyListener(ij);
panel.add(applyB);
resetB = new TrimmedButton("Reset",trim);
resetB.addActionListener(this);
resetB.addKeyListener(ij);
panel.add(resetB);
setB = new TrimmedButton("Set",trim);
setB.addActionListener(this);
setB.addKeyListener(ij);
panel.add(setB);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(0, 5, 10, 5);
add(panel, c);
addKeyListener(ij); // ImageJ handles keyboard shortcuts
pack();
Point loc = Prefs.getLocation(LOC_KEY);
if (loc!=null)
setLocation(loc);
else
GUI.center(this);
if (IJ.isMacOSX()) setResizable(false);
show();
thread = new Thread(this, "ThresholdAdjuster");
//thread.setPriority(thread.getPriority()-1);
thread.start();
ImagePlus imp = WindowManager.getCurrentImage();
ImagePlus.addImageListener(this);
if (imp!=null)
setup(imp, true);
}
public synchronized void adjustmentValueChanged(AdjustmentEvent e) {
if (e.getSource()==minSlider)
minValue = minSlider.getValue();
else
maxValue = maxSlider.getValue();
notify();
}
public synchronized void actionPerformed(ActionEvent e) {
Button b = (Button)e.getSource();
if (b==null) return;
if (b==resetB)
doReset = true;
else if (b==autoB)
doAutoAdjust = true;
else if (b==applyB)
doApplyLut = true;
else if (b==setB)
doSet = true;
notify();
}
public void imageUpdated(ImagePlus imp) {
if (imp.getID() == previousImageID && Thread.currentThread() != thread)
imageWasUpdated = true;
}
public void imageOpened(ImagePlus imp) {}
public void imageClosed(ImagePlus imp) {}
void setLutColor(int mode) {
switch (mode) {
case RED:
lutColor = ImageProcessor.RED_LUT;
break;
case BLACK_AND_WHITE:
lutColor = ImageProcessor.BLACK_AND_WHITE_LUT;
break;
case OVER_UNDER:
lutColor = ImageProcessor.OVER_UNDER_LUT;
break;
}
}
public synchronized void itemStateChanged(ItemEvent e) {
Object source = e.getSource();
if (source==methodChoice) {
method = methodChoice.getSelectedItem();
doAutoAdjust = true;
} else if (source==modeChoice) {
mode = modeChoice.getSelectedIndex();
setLutColor(mode);
doStateChange = true;
} else
doAutoAdjust = true;
notify();
}
ImageProcessor setup(ImagePlus imp, boolean enableAutoThreshold) {
ImageProcessor ip;
int type = imp.getType();
if (type==ImagePlus.COLOR_RGB || (imp.isComposite()&&((CompositeImage)imp).getMode()==IJ.COMPOSITE))
return null;
ip = imp.getProcessor();
boolean minMaxChange = false;
boolean not8Bits = type==ImagePlus.GRAY16 || type==ImagePlus.GRAY32;
int slice = imp.getCurrentSlice();
if (not8Bits) {
if (ip.getMin()==plot.stackMin && ip.getMax()==plot.stackMax && !imageWasUpdated)
minMaxChange = false;
else if (ip.getMin()!=previousMin || ip.getMax()!=previousMax || imageWasUpdated) {
minMaxChange = true;
previousMin = ip.getMin();
previousMax = ip.getMax();
} else if (slice!=previousSlice)
minMaxChange = true;
}
int id = imp.getID();
int roiHashCode = roiHashCode(imp.getRoi());
if (minMaxChange || id!=previousImageID || type!=previousImageType ||
imageWasUpdated || roiHashCode!=previousRoiHashCode) {
minThreshold = ip.getMinThreshold();
maxThreshold = ip.getMaxThreshold();
boolean isThresholded = minThreshold != ImageProcessor.NO_THRESHOLD
&& ip.getCurrentColorModel() != ip.getColorModel(); //does not work???
//IJ.log("Changed: min/max:"+minMaxChange +" id:"+ (id!=previousImageID)+" type:"+(type!=previousImageType)+" updated:"+imageWasUpdated+". isThresh="+isThresholded);
//IJ.log(minThreshold+"..."+maxThreshold);
//Undo.reset(); removed 2014-02-06 M. Schmid - why was it there?
if (not8Bits && minMaxChange && !isThresholded)
ip.resetMinAndMax(); //imp.updateAndDraw() is below
ImageStatistics stats = plot.setHistogram(imp, entireStack(imp));
if (stats == null)
return null;
if (isThresholded) {
minThreshold = scaleDown(ip, minThreshold);
maxThreshold = scaleDown(ip, maxThreshold);
} else {
if (enableAutoThreshold && !isThresholded)
autoSetLevels(ip, stats);
else
minThreshold = ImageProcessor.NO_THRESHOLD; //may be an invisible threshold after 'apply'
}
scaleUpAndSet(ip, minThreshold, maxThreshold);
updateLabels(imp, ip);
updatePercentiles(imp, ip);
updatePlot(ip);
updateScrollBars();
imp.updateAndDraw();
imageWasUpdated = false;
}
previousImageID = id;
previousImageType = type;
previousRoiHashCode = roiHashCode;
previousSlice = slice;
firstActivation = false;
return ip;
}
boolean entireStack(ImagePlus imp) {
return stackHistogram!=null && stackHistogram.getState() && imp.getStackSize()>1;
}
void autoSetLevels(ImageProcessor ip, ImageStatistics stats) {
if (stats==null || stats.histogram==null) {
minThreshold = defaultMinThreshold;
maxThreshold = defaultMaxThreshold;
return;
}
//int threshold = ip.getAutoThreshold(stats.histogram);
boolean darkb = darkBackground!=null && darkBackground.getState();
boolean invertedLut = ip.isInvertedLut();
int modifiedModeCount = stats.histogram[stats.mode];
if (!method.equals(methodNames[DEFAULT]))
stats.histogram[stats.mode] = plot.originalModeCount;
int threshold = thresholder.getThreshold(method, stats.histogram);
stats.histogram[stats.mode] = modifiedModeCount;
if (darkb) {
if (invertedLut)
{minThreshold=0; maxThreshold=threshold;}
else
{minThreshold=threshold+1; maxThreshold=255;}
} else {
if (invertedLut)
{minThreshold=threshold+1; maxThreshold=255;}
else
{minThreshold=0; maxThreshold=threshold;}
}
if (minThreshold>255) minThreshold = 255;
if (Recorder.record) {
boolean stack = stackHistogram!=null && stackHistogram.getState();
String options = method+(darkb?" dark":"")+(stack?" stack":"");
if (Recorder.scriptMode())
Recorder.recordCall("IJ.setAutoThreshold(imp, \""+options+"\");");
else
Recorder.record("setAutoThreshold", options);
}
}
/** Scales threshold levels in the range 0-255 to the actual levels. */
void scaleUpAndSet(ImageProcessor ip, double minThreshold, double maxThreshold) {
if (!(ip instanceof ByteProcessor) && minThreshold!=ImageProcessor.NO_THRESHOLD) {
double min = ip.getMin();
double max = ip.getMax();
if (max>min) {
minThreshold = min + (minThreshold/255.0)*(max-min);
maxThreshold = min + (maxThreshold/255.0)*(max-min);
} else
minThreshold = maxThreshold = min;
}
ip.setThreshold(minThreshold, maxThreshold, lutColor);
//ip.setSnapshotPixels(null); // disable undo removed 20140206 M. Schmid
}
/** Scales a threshold level to the range 0-255. */
double scaleDown(ImageProcessor ip, double threshold) {
if (ip instanceof ByteProcessor)
return threshold;
double min = ip.getMin();
double max = ip.getMax();
if (max>min)
return ((threshold-min)/(max-min))*255.0;
else
return ImageProcessor.NO_THRESHOLD;
}
/** Scales a threshold level in the range 0-255 to the actual level. */
double scaleUp(ImageProcessor ip, double threshold) {
double min = ip.getMin();
double max = ip.getMax();
if (max>min)
return min + (threshold/255.0)*(max-min);
else
return ImageProcessor.NO_THRESHOLD;
}
void updatePlot(ImageProcessor ip) {
plot.minThreshold = ip.getMinThreshold()==ImageProcessor.NO_THRESHOLD ?
-1 : (int)Math.round(minThreshold);
plot.maxThreshold = (int)Math.round(maxThreshold);
plot.mode = mode;
plot.repaint();
}
void updatePercentiles(ImagePlus imp, ImageProcessor ip) {
if (percentiles==null)
return;
ImageStatistics stats = plot.stats;
int minThresholdInt = (int)Math.round(minThreshold);
int maxThresholdInt = (int)Math.round(maxThreshold);
if (stats != null && stats.histogram != null && stats.histogram.length==256 &&
ip.getMinThreshold()!=ImageProcessor.NO_THRESHOLD &&
minThresholdInt>=0 && minThresholdInt<256 && maxThresholdInt>=0 && maxThresholdInt<256) {
int[] histogram = stats.histogram;
int below = 0, inside = 0, above = 0;
int minValue=0, maxValue=255;
if (imp.getBitDepth()==16 && !entireStack(imp)) {
ip.setRoi(imp.getRoi());
histogram = ip.getHistogram();
minThresholdInt = (int)Math.round(ip.getMinThreshold());
maxThresholdInt = (int)Math.round(ip.getMaxThreshold());
minValue=(int)ip.getMin(); maxValue=(int)ip.getMax();
}
for (int i=minValue; i<minThresholdInt; i++)
below += histogram[i];
for (int i=minThresholdInt; i<=maxThresholdInt; i++)
inside += histogram[i];
for (int i=maxThresholdInt+1; i<maxValue; i++)
above += histogram[i];
int total = below + inside + above;
//IJ.log("<"+minThresholdInt+":"+below+" in:"+inside+"; >"+maxThresholdInt+":"+above+" sum="+total);
int digits = imp.getCalibration()!=null || (ip instanceof FloatProcessor) ?
Math.max(Analyzer.getPrecision(), 2) : 0;
if (mode==OVER_UNDER)
percentiles.setText("below: "+IJ.d2s(100.*below/total)+" %, above: "+IJ.d2s(100.*above/total)+" %");
else
percentiles.setText(IJ.d2s(100.*inside/total)+" %");
} else
percentiles.setText("");
}
void updateLabels(ImagePlus imp, ImageProcessor ip) {
if (label1==null || label2==null)
return;
double min = ip.getMinThreshold();
double max = ip.getMaxThreshold();
if (min==ImageProcessor.NO_THRESHOLD) {
label1.setText("");
label2.setText("");
} else {
Calibration cal = imp.getCalibration();
if (cal.calibrated()) {
min = cal.getCValue((int)min);
max = cal.getCValue((int)max);
}
if (((int)min==min && (int)max==max) || (ip instanceof ShortProcessor) || max>99999.0) {
label1.setText(""+(int)min);
label2.setText(""+(int)max);
} else {
label1.setText(""+IJ.d2s(min,2));
label2.setText(""+IJ.d2s(max,2));
}
}
}
void updateScrollBars() {
minSlider.setValue((int)minThreshold);
maxSlider.setValue((int)maxThreshold);
}
/** Restore image outside non-rectangular roi. */
void doMasking(ImagePlus imp, ImageProcessor ip) {
ImageProcessor mask = imp.getMask();
if (mask!=null)
ip.reset(mask);
}
void adjustMinThreshold(ImagePlus imp, ImageProcessor ip, double value) {
if (IJ.altKeyDown() || IJ.shiftKeyDown() ) {
double width = maxThreshold-minThreshold;
if (width<1.0) width = 1.0;
minThreshold = value;
maxThreshold = minThreshold+width;
if ((minThreshold+width)>255) {
minThreshold = 255-width;
maxThreshold = minThreshold+width;
minSlider.setValue((int)minThreshold);
}
maxSlider.setValue((int)maxThreshold);
scaleUpAndSet(ip, minThreshold, maxThreshold);
return;
}
minThreshold = value;
if (maxThreshold<minThreshold) {
maxThreshold = minThreshold;
maxSlider.setValue((int)maxThreshold);
}
scaleUpAndSet(ip, minThreshold, maxThreshold);
}
void adjustMaxThreshold(ImagePlus imp, ImageProcessor ip, int cvalue) {
maxThreshold = cvalue;
if (minThreshold>maxThreshold) {
minThreshold = maxThreshold;
minSlider.setValue((int)minThreshold);
}
if (minThreshold < 0) { //remove NO_THRESHOLD
minThreshold = 0;
minSlider.setValue((int)minThreshold);
}
scaleUpAndSet(ip, minThreshold, maxThreshold);
IJ.setKeyUp(KeyEvent.VK_ALT);
IJ.setKeyUp(KeyEvent.VK_SHIFT);
}
void reset(ImagePlus imp, ImageProcessor ip) {
ip.resetThreshold();
ImageStatistics stats = plot.setHistogram(imp, entireStack(imp));
if (!(ip instanceof ByteProcessor)) {
if (entireStack(imp))
ip.setMinAndMax(stats.min, stats.max);
else
ip.resetMinAndMax();
}
updateScrollBars();
if (Recorder.record) {
if (Recorder.scriptMode())
Recorder.recordCall("IJ.resetThreshold(imp);");
else
Recorder.record("resetThreshold");
}
}
void doSet(ImagePlus imp, ImageProcessor ip) {
double level1 = ip.getMinThreshold();
double level2 = ip.getMaxThreshold();
if (level1==ImageProcessor.NO_THRESHOLD) {
level1 = scaleUp(ip, defaultMinThreshold);
level2 = scaleUp(ip, defaultMaxThreshold);
}
Calibration cal = imp.getCalibration();
int digits = (ip instanceof FloatProcessor)||cal.calibrated()?2:0;
level1 = cal.getCValue(level1);
level2 = cal.getCValue(level2);
GenericDialog gd = new GenericDialog("Set Threshold Levels");
gd.addNumericField("Lower Threshold Level: ", level1, digits);
gd.addNumericField("Upper Threshold Level: ", level2, digits);
gd.showDialog();
if (gd.wasCanceled())
return;
level1 = gd.getNextNumber();
level2 = gd.getNextNumber();
level1 = cal.getRawValue(level1);
level2 = cal.getRawValue(level2);
if (level2<level1)
level2 = level1;
double minDisplay = ip.getMin();
double maxDisplay = ip.getMax();
ip.resetMinAndMax();
double minValue = ip.getMin();
double maxValue = ip.getMax();
if (level1<minValue) level1 = minValue;
if (level2>maxValue) level2 = maxValue;
IJ.wait(500);
ip.setThreshold(level1, level2, lutColor);
ip.setSnapshotPixels(null); // disable undo
setup(imp, false);
if (Recorder.record) {
if (imp.getBitDepth()==32) {
if (Recorder.scriptMode())
Recorder.recordCall("IJ.setThreshold("+ip.getMinThreshold()+", "+ip.getMaxThreshold()+");");
else
Recorder.record("setThreshold", ip.getMinThreshold(), ip.getMaxThreshold());
} else {
int min = (int)ip.getMinThreshold();
int max = (int)ip.getMaxThreshold();
if (cal.isSigned16Bit()) {
min = (int)cal.getCValue(level1);
max = (int)cal.getCValue(level2);
}
if (Recorder.scriptMode())
Recorder.recordCall("IJ.setThreshold(imp, "+min+", "+max+");");
else
Recorder.record("setThreshold", min, max);
}
}
}
void changeState(ImagePlus imp, ImageProcessor ip) {
scaleUpAndSet(ip, minThreshold, maxThreshold);
updateScrollBars();
}
void autoThreshold(ImagePlus imp, ImageProcessor ip) {
ip.resetThreshold();
previousImageID = 0;
setup(imp, true);
}
void apply(ImagePlus imp) {
try {
if (imp.getBitDepth()==32) {
GenericDialog gd = new GenericDialog("NaN Backround");
gd.addCheckbox("Set Background Pixels to NaN", backgroundToNaN);
gd.showDialog();
if (gd.wasCanceled()) {
runThresholdCommand();
return;
}
backgroundToNaN = gd.getNextBoolean();
if (backgroundToNaN) {
Recorder.recordInMacros = true;
IJ.run("NaN Background");
Recorder.recordInMacros = false;
} else
runThresholdCommand();
} else
runThresholdCommand();
} catch (Exception e)
{/* do nothing */}
//close();
}
void runThresholdCommand() {
Thresholder.setMethod(method);
Thresholder.setBackground(darkBackground.getState()?"Dark":"Light");
if (Recorder.record) {
Recorder.setCommand("Convert to Mask");
(new Thresholder()).run("mask");
Recorder.saveCommand();
} else
(new Thresholder()).run("mask");
}
static final int RESET=0, AUTO=1, HIST=2, APPLY=3, STATE_CHANGE=4, MIN_THRESHOLD=5, MAX_THRESHOLD=6, SET=7;
// Separate thread that does the potentially time-consuming processing
public void run() {
while (!done) {
synchronized(this) {
try {wait();}
catch(InterruptedException e) {}
}
doUpdate();
}
}
void doUpdate() {
ImagePlus imp;
ImageProcessor ip;
int action;
int min = minValue;
int max = maxValue;
if (doReset) action = RESET;
else if (doAutoAdjust) action = AUTO;
else if (doApplyLut) action = APPLY;
else if (doStateChange) action = STATE_CHANGE;
else if (doSet) action = SET;
else if (minValue>=0) action = MIN_THRESHOLD;
else if (maxValue>=0) action = MAX_THRESHOLD;
else return;
minValue = -1;
maxValue = -1;
doReset = false;
doAutoAdjust = false;
doApplyLut = false;
doStateChange = false;
doSet = false;
imp = WindowManager.getCurrentImage();
if (imp==null) {
IJ.beep();
IJ.showStatus("No image");
return;
}
ip = setup(imp, false);
if (ip==null) {
imp.unlock();
IJ.beep();
if (imp.isComposite())
IJ.showStatus("\"Composite\" mode images cannot be thresholded");
else
IJ.showStatus("RGB images cannot be thresholded");
return;
}
//IJ.write("setup: "+(imp==null?"null":imp.getTitle()));
switch (action) {
case RESET: reset(imp, ip); break;
case AUTO: autoThreshold(imp, ip); break;
case APPLY: apply(imp); break;
case STATE_CHANGE: changeState(imp, ip); break;
case SET: doSet(imp, ip); break;
case MIN_THRESHOLD: adjustMinThreshold(imp, ip, min); break;
case MAX_THRESHOLD: adjustMaxThreshold(imp, ip, max); break;
}
updatePlot(ip);
updateLabels(imp, ip);
updatePercentiles(imp, ip);
ip.setLutAnimation(true);
imp.updateAndDraw();
}
/** Overrides close() in PlugInFrame. */
public void close() {
super.close();
instance = null;
done = true;
Prefs.saveLocation(LOC_KEY, getLocation());
Prefs.set(MODE_KEY, mode);
Prefs.set(DARK_BACKGROUND, darkBackground.getState());
synchronized(this) {
notify();
}
}
public void windowActivated(WindowEvent e) {
super.windowActivated(e);
plot.requestFocus();
ImagePlus imp = WindowManager.getCurrentImage();
if (!firstActivation && imp!=null)
setup(imp, false);
}
// Returns a hashcode for the specified ROI that typically changes
// if it is moved, even though is still the same object.
int roiHashCode(Roi roi) {
return roi!=null?roi.getHashCode():0;
}
/** Notifies the ThresholdAdjuster that the image has changed.
* If the image has no threshold, it does not autothreshold the image. */
public static void update() {
if (instance!=null) {
ThresholdAdjuster ta = ((ThresholdAdjuster)instance);
ImagePlus imp = WindowManager.getCurrentImage();
if (imp!=null && ta.previousImageID==imp.getID()) {
ta.previousImageID = 0;
ta.setup(imp, false);
}
}
}
/** Returns the current method ("Default", "Huang", etc). */
public static String getMethod() {
return method;
}
/** Returns the current mode ("Red","B&W" or"Over/Under"). */
public static String getMode() {
return modes[mode];
}
} // ThresholdAdjuster class
class ThresholdPlot extends Canvas implements Measurements, MouseListener {
static final int WIDTH = 256, HEIGHT=48;
int minThreshold = -1;
int maxThreshold = 170;
ImageStatistics stats;
int[] histogram;
Color[] hColors;
int hmax;
Image os;
Graphics osg;
int mode;
int originalModeCount;
double stackMin, stackMax;
int imageID2;
boolean entireStack2;
double mean2;
public ThresholdPlot() {
addMouseListener(this);
setSize(WIDTH+2, HEIGHT+2);
}
/** Overrides Component getPreferredSize(). Added to work
around a bug in Java 1.4.1 on Mac OS X.*/
public Dimension getPreferredSize() {
return new Dimension(WIDTH+2, HEIGHT+2);
}
ImageStatistics setHistogram(ImagePlus imp, boolean entireStack) {
double mean = entireStack?imp.getProcessor().getStatistics().mean:0.0;
if (entireStack && stats!=null && imp.getID()==imageID2
&& entireStack==entireStack2 && mean==mean2)
return stats;
mean2 = mean;
ImageProcessor ip = imp.getProcessor();
ColorModel cm = ip.getColorModel();
stats = null;
if (entireStack) {
if (imp.isHyperStack()) {
ImageStack stack = ChannelSplitter.getChannel(imp, imp.getChannel());
stats = new StackStatistics(new ImagePlus("", stack));
} else
stats = new StackStatistics(imp);
}
if (!(ip instanceof ByteProcessor)) {
if (entireStack) {
if (imp.getLocalCalibration().isSigned16Bit())
{stats.min += 32768; stats.max += 32768;}
stackMin = stats.min;
stackMax = stats.max;
ip.setMinAndMax(stackMin, stackMax);
imp.updateAndDraw();
} else {
stackMin = stackMax = 0.0;
if (entireStack2) {
ip.resetMinAndMax();
imp.updateAndDraw();
}
}
Calibration cal = imp.getCalibration();
if (ip instanceof FloatProcessor) {
int digits = Math.max(Analyzer.getPrecision(), 2);
IJ.showStatus("min="+IJ.d2s(ip.getMin(),digits)+", max="+IJ.d2s(ip.getMax(),digits));
} else
IJ.showStatus("min="+(int)cal.getCValue(ip.getMin())+", max="+(int)cal.getCValue(ip.getMax()));
ip = ip.convertToByte(true);
ip.setColorModel(ip.getDefaultColorModel());
}
Roi roi = imp.getRoi();
if (roi!=null && !roi.isArea()) roi = null;
ip.setRoi(roi);
if (stats==null)
stats = ImageStatistics.getStatistics(ip, AREA+MIN_MAX+MODE, null);
int maxCount2 = 0;
histogram = stats.histogram;
originalModeCount = histogram[stats.mode];
for (int i = 0; i < stats.nBins; i++)
if ((histogram[i] > maxCount2) && (i != stats.mode))
maxCount2 = histogram[i];
hmax = stats.maxCount;
if ((hmax>(maxCount2 * 2)) && (maxCount2 != 0))
hmax = (int)(maxCount2 * 1.5);
os = null;
if (!(cm instanceof IndexColorModel))
return null;
IndexColorModel icm = (IndexColorModel)cm;
int mapSize = icm.getMapSize();
if (mapSize!=256)
return null;
byte[] r = new byte[256];
byte[] g = new byte[256];
byte[] b = new byte[256];
icm.getReds(r);
icm.getGreens(g);
icm.getBlues(b);
hColors = new Color[256];
final int brightnessLimit = 1800; // 0 ... 2550 scale; brightness is reduced above
for (int i=0; i<256; i++) { //avoid colors that are too bright (invisible)
int sum = 4*(r[i]&255) + 5*(g[i]&255) + (b[i]&255);
if (sum > brightnessLimit) {
r[i] = (byte)(((r[i]&255)*brightnessLimit*2)/(sum+brightnessLimit));
g[i] = (byte)(((g[i]&255)*brightnessLimit*2)/(sum+brightnessLimit));
b[i] = (byte)(((b[i]&255)*brightnessLimit*2)/(sum+brightnessLimit));
}
hColors[i] = new Color(r[i]&255, g[i]&255, b[i]&255);
}
imageID2 = imp.getID();
entireStack2 = entireStack;
return stats;
}
public void update(Graphics g) {
paint(g);
}
public void paint(Graphics g) {
if (g==null) return;
if (histogram!=null) {
if (os==null && hmax>0) {
os = createImage(WIDTH,HEIGHT);
osg = os.getGraphics();
osg.setColor(Color.white);
osg.fillRect(0, 0, WIDTH, HEIGHT);
osg.setColor(Color.gray);
for (int i = 0; i < WIDTH; i++) {
if (hColors!=null) osg.setColor(hColors[i]);
int histValue = histogram[i]<hmax ? histogram[i] : hmax;
osg.drawLine(i, HEIGHT, i, HEIGHT - (HEIGHT*histogram[i]+hmax-1)/hmax);
}
osg.dispose();
}
if (os==null) return;
g.drawImage(os, 1, 1, this);
} else {
g.setColor(Color.white);
g.fillRect(1, 1, WIDTH, HEIGHT);
}
g.setColor(Color.black);
g.drawRect(0, 0, WIDTH+1, HEIGHT+1);
if (minThreshold>=0 && minThreshold<=255 && maxThreshold>=0 && maxThreshold<=255) {
if (mode==ThresholdAdjuster.OVER_UNDER) {
g.setColor(Color.blue);
g.drawRect(0, 0, minThreshold, HEIGHT+1);
g.drawRect(0, 1, minThreshold, 1);
g.setColor(Color.green);
g.drawRect(maxThreshold+2, 0, WIDTH-maxThreshold-1, HEIGHT+1);
g.drawLine(maxThreshold+2, 1, WIDTH+1,1);
return;
}
if (mode==ThresholdAdjuster.RED)
g.setColor(Color.red);
g.drawRect(minThreshold+1, 0, maxThreshold-minThreshold, HEIGHT+1);
g.drawLine(minThreshold+1, 1, maxThreshold+1, 1);
}
}
public void mousePressed(MouseEvent e) {}
public void mouseReleased(MouseEvent e) {}
public void mouseExited(MouseEvent e) {}
public void mouseClicked(MouseEvent e) {}
public void mouseEntered(MouseEvent e) {}
} // ThresholdPlot class