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Summary

Analysis run by Snakefile using this config file. See the README in the top directory for details.

Here is the rule graph of the computational workflow: rulegraph.svg

Here is the Markdown output of each notebook in the workflow:

  1. Count variants by barcode. Creates a variant counts file giving counts of each barcoded variant in each sample.

  2. Compute escape fractions for individual barcodes.

  3. Determine homolog escape fraction averaged across all barcodes. Generates summary plots including heatmaps.

  4. For antibodies with more quantitative titration escape, determine homolog EC50s averaged across all barcodes. Generates summary plots including heatmaps.