Analysis run by Snakefile using this config file. See the README in the top directory for details.
Here is the rule graph of the computational workflow:
Here is the Markdown output of each notebook in the workflow:
-
Count variants by barcode. Creates a variant counts file giving counts of each barcoded variant in each sample.
-
Compute escape fractions for individual barcodes.
-
Determine homolog escape fraction averaged across all barcodes. Generates summary plots including heatmaps.
-
For antibodies with more quantitative titration escape, determine homolog EC50s averaged across all barcodes. Generates summary plots including heatmaps.