Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

qc_read_collection() error: Can't combine <double> and <character> #22

Open
scrameri opened this issue Dec 21, 2021 · 0 comments
Open

Comments

@scrameri
Copy link

Dear Alboukadel,

Many thanks for this and other handy R packages!

I've beein using qc_read_collection(), on many "*_fastqc.zip" files, and noticed that this function suffers from dplyr issue #5358 when binding data.frames.

Here is a reprex leading to the error in lapply(res, dplyr::bind_rows, .id = "sample") inside qc_read_collection():

library(dplyr)
#> 
#> Attaching package: 'dplyr'
#> The following objects are masked from 'package:stats':
#> 
#>     filter, lag
#> The following objects are masked from 'package:base':
#> 
#>     intersect, setdiff, setequal, union

# create example data.frames to be bound using dplyr::bind_rows()
dn <- data.frame(Length = 150, Count = 2)
ds <- data.frame(Length = c("150-155"), Count = 4)
de <- data.frame(array(NA, dim = c(0,0)))
res <- list(module = list(Sample1=dn, Sample2=ds, Sample3=de))

str(res)
#> List of 1
#>  $ module:List of 3
#>   ..$ Sample1:'data.frame':  1 obs. of  2 variables:
#>   .. ..$ Length: num 150
#>   .. ..$ Count : num 2
#>   ..$ Sample2:'data.frame':  1 obs. of  2 variables:
#>   .. ..$ Length: chr "150-155"
#>   .. ..$ Count : num 4
#>   ..$ Sample3:'data.frame':  0 obs. of  0 variables

# reproduce the error
res <- lapply(res, dplyr::bind_rows, .id = "sample")
#> Error: Can't combine `Sample1$Length` <double> and `Sample2$Length` <character>.

The error above will occur when calling qc_read_collection(files, sample_names, modules = "all") on a collection of "*_fastqc.zip" files, if there is a sample in files that has a different class for any variable in the data.frame to be bound.

In my case, this happened mostly with the modules $sequence_length_distribution (variable "Length") or $kmer_content (variable "Max Obs/Exp Position").

Here is a possible fix I came up with:

# convert <double> to <character> if a column should be <character>
res <- lapply(res, function(x) {
  # tibble with classes for each non-emtpy data.frame column
  dcl <- dplyr::bind_rows(lapply(x, function(y) {
    if (nrow(y) > 0) sapply(y, class)
  }))
  # define classes to assign
  cl <- apply(dcl, 2, function(z) ifelse(any(z=="character"),"character",z[1]))
  # assign classes
  lapply(x, function(w) {
    if (nrow(w) > 0) {for (i in names(w)) {class(w[,i]) <- cl[i]} ; w}
  })
})

# reproduce the fix
res <- lapply(res, dplyr::bind_rows, .id = "sample")
str(res)
#> List of 1
#>  $ module:'data.frame':  2 obs. of  3 variables:
#>   ..$ sample: chr [1:2] "Sample1" "Sample2"
#>   ..$ Length: chr [1:2] "150" "150-155"
#>   ..$ Count : num [1:2] 2 4

Created on 2021-12-21 by the reprex package (v2.0.1)

Perhaps a patch for qc_read_collection() similar to the one below (enclosed by ##<##<##) could be useful generally, given that dplyr is not going to fix this because it is a "deliberate design decision" (see #5358)?

qc_read_collection <- function(files, sample_names, modules = "all", verbose=T) 
{
  module_data <- lapply(files, qc_read, modules = modules, 
                        verbose = verbose)
  if (missing(sample_names) || length(sample_names) != length(files)) {
    sample_names <- lapply(module_data, function(x) unique(x$summary))
    sample_names <- unlist(sample_names)
  }
  names(module_data) <- sample_names
  module_names <- unique(unlist(lapply(module_data, names)))
  res <- list()
  for (i in seq_along(module_names)) {
    res[[i]] <- lapply(module_data, function(x) as.data.frame(x[[module_names[i]]]))
  }
  names(res) <- module_names
  
  ##<##<## begin patch
  res <- lapply(res, function(x) {
    dcl <- dplyr::bind_rows(lapply(x, function(y) {
      if (nrow(y) > 0) sapply(y, class)
      }))
    cl <- apply(dcl, 2, function(z) ifelse(any(z=="character"),"character",z[1]))
    lapply(x, function(w) {
      if (nrow(w) > 0) {for (i in names(w)) {class(w[,i]) <- cl[i]} ; w}
    })
  })
  ##<##<## end patch
  
  res <- lapply(res, dplyr::bind_rows, .id = "sample")
  res <- structure(res, class = c("list", "qc_read_collection"))
  res
}

Perhaps you'd like to look into this yourself, and maybe come up with an easier and prettier solution? :)

Cheers,
Simon

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant