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Our lab is doing research on some channel protein, the sequencing error seems different from R9 pore, so we want to training marginpolish/Helen with these new data, could you tell me how to do it, thanks.
The text was updated successfully, but these errors were encountered:
This sounds very interesting, and thanks for choosing MP/HELEN for your experiment. If this is a new channel and the error mode is different, I'd suggest you try PEPPER (https://github.com/kishwarshafin/pepper) to train and test your new pore. The reason would be simplicity and a faster trial-error loop. If you open an issue there or send me an email at shafin@ucsc.edu, I can help you with setting up a training run.
Things you need to train a polisher:
A truth assembly (high-quality assembly that can be regarded as the truth)
A draft assembly (an assembly that you are trying to improve)
Reads aligned to the draft assembly.
If you have these, open an issue in PEPPER repository and I will help you with that.
Our lab is doing research on some channel protein, the sequencing error seems different from R9 pore, so we want to training marginpolish/Helen with these new data, could you tell me how to do it, thanks.
The text was updated successfully, but these errors were encountered: