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02_basecalling.md

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02 - Basecalling

Created: 2022/08/11 11:02:25 Last modified: 2022/08/18 13:15:33

  • Aim: This document documents/describes basecalling for this project
  • Prerequisite software: slurm v20.11.6, GNU coreutils
  • OS: ORAC (CentOS Linux) (ESR production network)

Table of contents

Basecalling

The flowcell and kit information used in the basecalling script was found in the sequencing report file report_FAQ91555_20220804_0852_3fb67c88.md

Run bash script to get the run basecalling. See my script at ./scripts/02_basecalling/01_basecalling.sh

sbatch ./scripts/02_basecalling/01_basecalling.sh

Merge fastq files

Run bash script to get the merge the fastq files. See my script at ./scripts/02_basecalling/02_merge_fastq.sh

sbatch ./scripts/02_basecalling/02_merge_fastq.sh