OIST Skill Pill on analyzing RNA-seq data, from raw reads to functional enrichment analysis.
2019 December 4, 5, 11, & 12; 3-5pm
Main lesson materials are in:
RNA-seq.md
&
R_RNA.Rmd
The tutorials are specific to working on the OIST HPC, Tombo, but could be adapted for use on other systems.
Softwares used:
- Fastqc
- Multiqc
- Trimmomatic
- Trinity
- Dammit
- Salmon
- R Studio
- DESeq2
- GOstats