Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

promethion ready - checklist #142

Open
5 tasks
WardDeb opened this issue Jan 3, 2024 · 1 comment
Open
5 tasks

promethion ready - checklist #142

WardDeb opened this issue Jan 3, 2024 · 1 comment
Assignees
Labels
enhancement New feature or request

Comments

@WardDeb
Copy link
Member

WardDeb commented Jan 3, 2024

Todo's to make the workflow 'promethion ready'. Related to #138, #139, #140

  • merge basecalled bam files (e.g. basecalled output)
  • fetch exact dorado (server) version + model used in MinKNOW and store this in the config / metadata to ship to end user. For MinKNOW v 23.07.12 the information in the json is confusing (e.g. guppy flags while dorado is used), the info in html should be correct.
  • basecalled bam files generated with minKNOW are not compatible with dorado align (v0.4.1 & 0.5.0).
  • basecalled bam files generated with minKNOW are not compatible with modbam2bed.
  • model_def & model are confusing in the metadata yaml (HAC vs SUP)
@WardDeb WardDeb added the enhancement New feature or request label Jan 3, 2024
@WardDeb
Copy link
Member Author

WardDeb commented Jan 31, 2024

per on site meeting:

  • parameters should be parsed from sequencingrun.html files rather then json/md
  • important to discriminate between minKNOW version/models & dorado models
  • bam header information should be parsed for consistency
  • explore expansion on epi2me's basecalling workflow for implementation into minKNOW

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
enhancement New feature or request
Projects
None yet
Development

No branches or pull requests

3 participants