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iu-merge-pairs error #3
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Hi, Can you please provide some example files? The first 1,000 lines of R1 and R2 reads for one of your samples in that dataset would be the best (i.e., Thanks, |
Thanks for your quick reply. I'm attaching the first 1000 lines. |
Hi, I found what causes the error and I will add a control when I have a chance for the next version. Clearly it should be illegal to use The reason you are forced to use
and this is how yours look like:
If you look at the specification on the Illumina page, you will realize that the format they describe matches to the first one: Best, |
Here is my case. I got my V1-V3 data sequenced by an external provider. They say they used the Illumina Casava pipeline version 1.8.3.
As a start I tried to generate a config file. I followed the steps listed at https://github.com/meren/illumina-utils:
I first generated a tab file listing all the sample names and the corresponding paired end fastq files. Than I ran iu-gen-configs and I was surprised that instead of generating a single config file it generated a config file for each sample.
Than I decided to merge the paired end fastq files for each sample by using iu-merge-pairs using the --compute-qual-dicts option. When I ran it for the first sample it produced the following error:
I added the parameter as requested. Than I got another error message:
I don't know what to do now. Can you, perhaps, advise?
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