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Everytime I try to run migmap on fastq files generated from pandaseq I get the following error
WARNING: An illegal reflective access operation has occurred
WARNING: Illegal reflective access by org.codehaus.groovy.reflection.CachedClass (file:/Users/aroederer/anaconda3/share/migmap.jar) to method java.lang.Object.finalize()
WARNING: Please consider reporting this to the maintainers of org.codehaus.groovy.reflection.CachedClass
WARNING: Use --illegal-access=warn to enable warnings of further illegal reflective access operations
WARNING: All illegal access operations will be denied in a future release
usage: migmap [options] input.(fa/fastq)[.gz] (output_file/- for stdout)
--all-alleles Will use all alleles during
alignment (this is going to be
slower). [default = use only major
(*01) alleles]
--allow-incomplete Report clonotypes with partial
CDR3 mapping.
--allow-no-cdr3 Report clonotypes with no CDR3
mapping.
--allow-noncanonical Report clonotypes that have
non-canonical CDR3 (do not start
with C or end with F/W residues).
--allow-noncoding Report clonotypes that have either
stop codon or frameshift in their
receptor sequence.
--blast-dir Path to folder that contains
'igblastn' and 'makeblastdb'
binaries. [default = assume they
are added to $PATH and execute
them directly]
--by-read Will output mapping details for
each read. [default = assemble
clonotypes and output clonotype
abundance table]
--custom-database Path to a custom segments
database. [default = use built-in
database]
--data-dir Path to folder that contains data
bundle (internal_data/ and
optional_file/ directories).
[default = $install_dir/data/]
--details <field1,field2,.../all> Additional fields to provide for
output, allowed values:
fr1nt,cdr1nt,fr2nt,cdr2nt,fr3nt,fr
4nt,contignt,fr1aa,cdr1aa,fr2aa,cd
r2aa,fr3aa,fr4aa,contigaa.
-h Display this help message
-n Number of reads to take. [default
= all]
-p Number of cores to use. [default =
all available processors]
-q <2..40> Threshold for average quality of
mutations and N-regions of CDR3
[default = 25]
-R <chain1,...> Receptor gene and chain. Several
chains can be specified, separated
with commas. Allowed values: [TRA,
TRB, TRG, TRD, IGH, IGL, IGK].
[required]
--report File to store MIGMAP report. Will
append report line if file exists.
-S Species. Allowed values: [human,
mouse, rat, rabbit,
rhesus_monkey]. [required]
--unmapped <fastq[.gz]> Output unmapped reads in specified
file.
--use-kabat Will use KABAT nomenclature for
CDR/FW partitioning. [default =
use IMGT nomenclature]
The text was updated successfully, but these errors were encountered:
Everytime I try to run migmap on fastq files generated from pandaseq I get the following error
WARNING: An illegal reflective access operation has occurred
WARNING: Illegal reflective access by org.codehaus.groovy.reflection.CachedClass (file:/Users/aroederer/anaconda3/share/migmap.jar) to method java.lang.Object.finalize()
WARNING: Please consider reporting this to the maintainers of org.codehaus.groovy.reflection.CachedClass
WARNING: Use --illegal-access=warn to enable warnings of further illegal reflective access operations
WARNING: All illegal access operations will be denied in a future release
usage: migmap [options] input.(fa/fastq)[.gz] (output_file/- for stdout)
--all-alleles Will use all alleles during
alignment (this is going to be
slower). [default = use only major
(*01) alleles]
--allow-incomplete Report clonotypes with partial
CDR3 mapping.
--allow-no-cdr3 Report clonotypes with no CDR3
mapping.
--allow-noncanonical Report clonotypes that have
non-canonical CDR3 (do not start
with C or end with F/W residues).
--allow-noncoding Report clonotypes that have either
stop codon or frameshift in their
receptor sequence.
--blast-dir Path to folder that contains
'igblastn' and 'makeblastdb'
binaries. [default = assume they
are added to $PATH and execute
them directly]
--by-read Will output mapping details for
each read. [default = assemble
clonotypes and output clonotype
abundance table]
--custom-database Path to a custom segments
database. [default = use built-in
database]
--data-dir Path to folder that contains data
bundle (internal_data/ and
optional_file/ directories).
[default = $install_dir/data/]
--details <field1,field2,.../all> Additional fields to provide for
output, allowed values:
fr1nt,cdr1nt,fr2nt,cdr2nt,fr3nt,fr
4nt,contignt,fr1aa,cdr1aa,fr2aa,cd
r2aa,fr3aa,fr4aa,contigaa.
-h Display this help message
-n Number of reads to take. [default
= all]
-p Number of cores to use. [default =
all available processors]
-q <2..40> Threshold for average quality of
mutations and N-regions of CDR3
[default = 25]
-R <chain1,...> Receptor gene and chain. Several
chains can be specified, separated
with commas. Allowed values: [TRA,
TRB, TRG, TRD, IGH, IGL, IGK].
[required]
--report File to store MIGMAP report. Will
append report line if file exists.
-S Species. Allowed values: [human,
mouse, rat, rabbit,
rhesus_monkey]. [required]
--unmapped <fastq[.gz]> Output unmapped reads in specified
file.
--use-kabat Will use KABAT nomenclature for
CDR/FW partitioning. [default =
use IMGT nomenclature]
The text was updated successfully, but these errors were encountered: