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Hello!
I have been trying to utilize some of the new features in the most recent release and am getting a lot of errors. I am analyzing non-human species and using a custom library. I am using App version: 4.5.0; built=Fri Sep 22 05:39:05 PDT 2023; rev=cdb24b4fb7; lib=repseqio.v3.0.1
The -nMutations/-aaMutations [and related commands]
I run the following command:
App version: 4.5.0; built=Fri Sep 22 05:39:05 PDT 2023; rev=cdb24b4fb7; lib=repseqio.v3.0.1
-nMutations: Alignment attached base gene features not allowed (error in VRegionTrimmed)
com.milaboratory.app.ValidationException: -nMutations: Alignment attached base gene features not allowed (error in VRegionTrimmed)
at com.milaboratory.o.lt.a(SourceFile:71)
at com.milaboratory.o.lt.a(SourceFile:52)
at com.milaboratory.o.rC.invoke(SourceFile:1568)
at com.milaboratory.o.kQ.validateParameters(SourceFile:49)
at com.milaboratory.mixcr.export.FieldWithParameters.create(SourceFile:314)
at com.milaboratory.o.kM.createFields(SourceFile:18)
at com.milaboratory.o.kX.a(SourceFile:80)
at com.milaboratory.mixcr.cli.CommandExportClones$Cmd.run1(SourceFile:241)
at com.milaboratory.mixcr.cli.MiXCRCommandWithOutputs.run0(SourceFile:69)
at com.milaboratory.mixcr.cli.MiXCRCommand.run(SourceFile:37)
at picocli.CommandLine.executeUserObject(CommandLine.java:1939)
at picocli.CommandLine.access$1300(CommandLine.java:145)
at picocli.CommandLine$RunLast.executeUserObjectOfLastSubcommandWithSameParent(CommandLine.java:2358)
at picocli.CommandLine$RunLast.handle(CommandLine.java:2352)
at picocli.CommandLine$RunLast.handle(CommandLine.java:2314)
at picocli.CommandLine$AbstractParseResultHandler.execute(CommandLine.java:2179)
at picocli.CommandLine$RunLast.execute(CommandLine.java:2316)
at com.milaboratory.mixcr.cli.Main.registerLogger$lambda-26(SourceFile:447)
at picocli.CommandLine.execute(CommandLine.java:2078)
at com.milaboratory.mixcr.cli.Main.main(SourceFile:98)
the -nMutationRate/-aaMutationRate
I run the following command: $mixcr exportClones -f --verbose --filter-out-of-frames --filter-stops -nFeature "{VEndTrimmed:DBeginTrimmed}" -nFeature "{DEndTrimmed:JBeginTrimmed}" -nMutationRate "{FR1Begin:VEndTrimmed}" -aaMutationRate "{FR1Begin:VEndTrimmed}" epfu.2007.5RACEbulk.TermSpleen.allelic.clns epfu.2007.5RACEbulk.TermSpleen.allelic.VRegionTrimmed.TEST.tsv
And I receive the following error:
the -nLength/-aaLength
I run the following command: mixcr exportClones -f --verbose --filter-out-of-frames --filter-stops -nFeature "{VEndTrimmed:DBeginTrimmed}" -nFeature "{DEndTrimmed:JBeginTrimmed}" -aaLength "{FR1Begin:VEndTrimmed}" -nLength "{FR1Begin:VEndTrimmed}" epfu.2007.5RACEbulk.TermSpleen.allelic.clns epfu.2007.5RACEbulk.TermSpleen.allelic.VRegionTrimmed.TEST.tsv
And I receive the following error:
Hello!
I have been trying to utilize some of the new features in the most recent release and am getting a lot of errors. I am analyzing non-human species and using a custom library. I am using App version: 4.5.0; built=Fri Sep 22 05:39:05 PDT 2023; rev=cdb24b4fb7; lib=repseqio.v3.0.1
I run the following command:
mixcr exportClones -f --verbose --filter-out-of-frames --filter-stops -nFeature "{VEndTrimmed:DBeginTrimmed}" -nFeature "{DEndTrimmed:JBeginTrimmed}" -nFeature "{FR1Begin:VEndTrimmed}" -nMutations "{FR1Begin:VEndTrimmed}" -aaFeature "{FR1Begin:VEndTrimmed}" -aaMutations "{FR1Begin:VEndTrimmed}" -baseBiochemicalProperties "VRegionTrimmed" epfu.2007.5RACEbulk.TermSpleen.allelic.clns epfu.2007.5RACEbulk.TermSpleen.allelic.VRegionTrimmed.tsv
And I receive the following error:
I run the following command:
$mixcr exportClones -f --verbose --filter-out-of-frames --filter-stops -nFeature "{VEndTrimmed:DBeginTrimmed}" -nFeature "{DEndTrimmed:JBeginTrimmed}" -nMutationRate "{FR1Begin:VEndTrimmed}" -aaMutationRate "{FR1Begin:VEndTrimmed}" epfu.2007.5RACEbulk.TermSpleen.allelic.clns epfu.2007.5RACEbulk.TermSpleen.allelic.VRegionTrimmed.TEST.tsv
And I receive the following error:
I run the following command:
mixcr exportClones -f --verbose --filter-out-of-frames --filter-stops -nFeature "{VEndTrimmed:DBeginTrimmed}" -nFeature "{DEndTrimmed:JBeginTrimmed}" -aaLength "{FR1Begin:VEndTrimmed}" -nLength "{FR1Begin:VEndTrimmed}" epfu.2007.5RACEbulk.TermSpleen.allelic.clns epfu.2007.5RACEbulk.TermSpleen.allelic.VRegionTrimmed.TEST.tsv
And I receive the following error:
Thanks for your help!
-Taylor
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