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Not an issue but questions #54

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FritzPeleke opened this issue Apr 16, 2020 · 2 comments
Open

Not an issue but questions #54

FritzPeleke opened this issue Apr 16, 2020 · 2 comments

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@FritzPeleke
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Hi I am new to NGS in general and I have a few may be dumb questions.

  • When should I use sickle se and when should I use pe?
    -Is it possible to run sickle like in a for loop so it trims like all my fastq files? If yes how
    -could you please elaborate more on its installation or direct me to a tutorial?
    Thanks :)
@pentalpha
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SE is for Single End fastq files and PE for Paired End.
A fastq (or just .fq) file is Paired End when, for each sample sequenced, there's a pair of files instead of just one.
Their names will usually be "something.1.fastq and something.2.fastq" or "something.L.fastq and something.R.fastq".

@FritzPeleke
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Thanks @pentalpha

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