Yeskit is an R package designed for single-cell gene expression data importation, integration, clustering, differential analysis, functional analysis, and visualization. Since Yeskit does not change the default data structure of Seurat, it can be easily integrated into most existing scRNA-seq analysis workflows.
Yeskit can be used to read other information (such as gene mutation-by-cell matrix, pathogen count-by-cell matrix) and store them as additional data in the Seurat obj@metadata slot. In addition, Yeskit also has the function of reading and distinguishing the source of cells from the scRNA-seq data of xenografts samples (PDX model).
When there are many points in the vector diagram, editing becomes difficult. To this end, most visualization functions in Yeskit have the option to rasterize the geom_point layer of ggplot2 and keep all axes, labels, and text in vector format.
Yeskit package can be easily installed under the following instruction:
devtools::install_github("ncrna/Yeskit")
Please check the following link to get a rapid understanding about Yeskit:
Walkthrough - Decoding Intracellular Pathogens of H3N2 at the Single-Cell level using Yeskit
In a particular case, the Cairo package can be loaded but crashes the R process when called. This is probably the case that the version of Cairo was built with an older version of R. Try the following instruction:
Bioconductor::install("Cairo", force = TRUE)
For OS X users, you may happen to an error like this:
Error : .onLoad failed in loadNamespace() for ‘Cairo’
...
Library not loaded: /opt/X11/lib/libXrender.1.dylib
The reason is X11 doesn't ship with OS X any more, users can download and install XQuartz from https://www.xquartz.org to solve this problem.
If you have any problems, comments or suggestions, please feel free to contact Wei Zhang, admin@ncrna.net.
Decoding Intracellular Pathogens of scRNA-seq experiments with PathogenTrack and Yeskit.