See the original ALPINE workflow repo here. This repo contains the in-development Rust source code meant to replace the core components of the ALPINE pipeline that were previously written in Julia. This tool has a rich command line interface intended to allow researchers to perform ALPINE-type analyses without invoking the entire Nextflow pipeline and associated Docker containers.
When you run alpine --help, the tool usage that comes up looks like this:
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ALPINE: Anachronistic Lineage and Persistent INfection Explorer
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Command line interface for the core Rust components of ALPINE.
These commands are called in the full pipeline, which is imple-
mented in Nextflow alongside `seqkit`, `csvtk`, `nushell`,
`vsearch`, and a bin of bespoke Python scripts. However, users
may also use the commands available in this crate to run
similar analyses themselves via the command line.
Usage: alpine [OPTIONS] [COMMAND]
Commands:
replace-gaps Replace FASTA gap symbols '-' with masked bases 'N'.
filter-by-n Filter out FASTA records with more than the desired number of masked 'N' bases.
separate-by-month Use collection dates from FASTA record metadata to sort all FASTA records into a separate FASTA for each year-month combination.
distance-matrix Compute a symmetric pairwise distance matrix based on how dissimilar sequences in the provided FASTA are to one another.
help Print this message or the help of the given subcommand(s)
Options:
-v, --verbose... Increase logging verbosity
-q, --quiet... Decrease logging verbosity
-t, --threads <THREADS> [default: 3]
-h, --help Print help
To set up and try out the toolset, either install the Rust toolchain and build it yourself or run ./easy_install in this directory after cloning it to your machine.