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MSI with Illumina paired end data #6

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Lveillat opened this issue Feb 14, 2023 · 1 comment
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MSI with Illumina paired end data #6

Lveillat opened this issue Feb 14, 2023 · 1 comment
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@Lveillat
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Hello,
Is it possible to use the MSI tool with Illumina paired-end data? If so, how should we proceed ?
Thank you.

@nunofonseca nunofonseca self-assigned this Mar 25, 2023
@nunofonseca
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Hi,
MSI was designed for long reads therefore it will not work with paired-end data.
However, if you really want to try MSI on your data then the paired reads would have to be merged before being provided to msi (as single reads). There are several tools available to merge reads: https://www.biostars.org/p/225683/
Cheers.

@nunofonseca nunofonseca added the question Further information is requested label Apr 7, 2023
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