Related schema specification: PatchClampVariation
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/cellAttachedPatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique in which the cell membrane remains intact.
- description
For this method, the pipette is sealed onto the cell membrane to obtain a gigaseal (a seal with electrical resistance on the order of a gigaohm), while ensuring that the cell membrane remains intact. This allows the recording of currents through single, or a few, ion channels contained in the patch of membrane captured by the pipette. By only attaching to the exterior of the cell membrane, there is very little disturbance of the cell structure. Also, by not disrupting the interior of the cell, any intracellular mechanisms normally influencing the channel will still be able to function as they would physiologically. [Wikipedia]
- name
cell-attached patch
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/insideOutPatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique in which a patch of membrane is excised and the cytosolic surface exposed.
- description
In the inside-out method, a patch of the membrane is attached to the patch pipette, detached from the rest of the cell, and the cytosolic surface of the membrane is exposed to the external media, or bath. One advantage of this method is that the experimenter has access to the intracellular surface of the membrane via the bath and can change the chemical composition of what the inside surface of the membrane is exposed to. This is useful when an experimenter wishes to manipulate the environment at the intracellular surface of single ion channels. [Wikipedia]
- name
inside-out patch
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/loosePatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique involving a seal with low electrical resistance.
- description
This technique employs a loose seal (low electrical resistance) rather than the tight gigaseal used in the conventional technique. A significant advantage of the loose seal is that the pipette can be repeatedly removed from the membrane after recording, and the membrane will remain intact. This allows repeated measurements in a variety of locations on the same cell without destroying the integrity of the membrane. [Wikipedia]
- name
loose patch
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/outsideOutPatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique in which a patch of membrane is excised and the external surface exposed.
- description
The name 'outside-out' emphasizes both this technique's complementarity to the inside-out technique, and the fact that it places the external rather than intracellular surface of the cell membrane on the outside of the patch of membrane, in relation to the patch electrode. Outside-out patching gives the experimenter the opportunity to examine the properties of an ion channel when it is isolated from the cell and exposed successively to different solutions on the extracellular surface of the membrane. [Wikipedia]
- name
outside-out patch
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/perforatedPatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique in which the cell membrane is perforated.
- description
This variation of the patch clamp method is very similar to the whole-cell configuration. The main difference lies in the fact that when the experimenter forms the gigaohm seal, suction is not used to rupture the patch membrane. Instead, the electrode solution contains small amounts of an antifungal or antibiotic agent which diffuses into the membrane patch and forms small pores in the membrane, providing electrical access to the cell interior. [Wikipedia]
- name
perforated patch
metadata sheet
- @id
https://openminds.ebrains.eu/instances/patchClampVariation/wholeCellPatch
- @type
https://openminds.ebrains.eu/controlledTerms/PatchClampVariation
- definition
A variation of the patch-clamp technique in which the patch is ruptured, giving access to the intracellular space.
- description
Whole-cell recordings involve recording currents through multiple channels simultaneously, over a large region of the cell membrane. The electrode is left in place on the cell, as in cell-attached recordings, but more suction is applied to rupture the membrane patch, thus providing access from the interior of the pipette to the intracellular space of the cell. This provides a means to administer and study how treatments (e.g. drugs) can affect cells in real time. [Wikipedia]
- name
whole-cell patch