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MITE-Hunter produces no results #12
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Dear Sanzhen, MITE-Hunter sometimes chokes and emits random errors. Normally a fresh rerun can solve such issues. You can just run the MITE module with Hope this helps! |
I just pushed some new updates to EDTA, mainly to fix the TIR-Learner issue. Please reinstall EDTA and rerun it in the same work folder. Existing results will be reused so there is essentially no waste of time. Thank you for your patience and support! |
Shujun, thank you very much. This helps. I will update and test it. |
Dear Shujun, Here is the screen output Sun Aug 18 03:38:01 MDT 2019 Dependency checking: Sun Aug 18 03:38:12 MDT 2019 Start to find LTR candidates. Sun Aug 18 03:38:12 MDT 2019 Identify LTR retrotransposon candidates from scratch. Sun Aug 18 04:53:51 MDT 2019 Finish finding LTR candidates. Sun Aug 18 04:53:51 MDT 2019 Start to find TIR candidates. Sun Aug 18 04:53:51 MDT 2019 Identify TIR candidates from scratch. Species: others Sun Aug 18 08:17:50 MDT 2019 Start to find MITE candidates. Sun Aug 18 08:17:50 MDT 2019 Identify MITE candidates from scratch. The cmd hanging there:
I tried to rerun this specific cmd, and the error reproduced. Thanks, |
My rerunning with the updated EDTA failed at the TIR step again. Will check in more details to see what was going on. |
Hi Sanzhen, Sorry the TIR issue is not resolved. Please let me know the specific error message so that I can work on it. Best, |
Shujun, here is the error message: Fri Aug 16 21:13:30 CDT 2019 Identify TIR candidates from scratch. No output was generated for Module2 or Module3. thanks, -Sanzhen |
Hi @liu3zhenlab, @QiushiLi and @philippbayer, I updated the TIR-Learner to v1.23 and hope to solve this issue. I also abandoned MITE-Hunter for its poor performance. Please update EDTA ( Best, |
Thanks for that! |
Thanks a lot Shujun. MITE-Hunter is also slow. Glad you found an alternative solution. |
No you don't have to. The change is to copy over TIR results directly and
pretend it's MITE result, which will be removed entirely in the next
combination step. MITE-Hunter does not contribute a lot (usually just a
couple sequences) so you won't loss too much of sensitivity.
…On Mon, Aug 26, 2019, 2:28 AM Philipp Bayer ***@***.***> wrote:
Thanks for that!
So I'm still running the split-up EDTA_raw.pl with the four different
types, after pulling the changes MITE just finishes immediately with the
MITE-Hunter warning.
Should I rerun the TIR step separately since that's where the changes seem
to have happened?
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Shujun, I tested the v1.5 with a small data set. It showed errors as: ######################################################## Extensive de-novo TE Annotator (EDTA) v1.5Shujun Ou (shujun.ou.1@gmail.com)######################################################## Mon Aug 26 12:33:52 CDT 2019 Dependency checking: Mon Aug 26 12:33:57 CDT 2019 Start to find LTR candidates. Mon Aug 26 12:33:57 CDT 2019 Identify LTR retrotransposon candidates from scratch.
cp: cannot stat ‘TF05-1v012.fasta.mod.retriever.scn.adj’: No such file or directory ERROR: Raw LTR results not found in TF05-1v012.fasta.EDTA.raw/TF05-1v012.fasta.LTR.raw.fa at /homes/liu3zhen/.conda/envs/EDTA3/EDTA/EDTA.pl line 176. |
Hi,shujun ################################################################# 2019年 08月 25日 星期日 17:07:57 CST Start to find LTR candidates. 2019年 08月 25日 星期日 17:07:57 CST Identify LTR retrotransposon candidates from scratch. 2019年 08月 25日 星期日 18:38:13 CST Finish finding LTR candidates. 2019年 08月 25日 星期日 18:38:13 CST Start to find TIR candidates. 2019年 08月 25日 星期日 18:38:13 CST Identify TIR candidates from scratch. Species: others 2019年 08月 25日 星期日 20:55:41 CST Start to find MITE candidates. 2019年 08月 25日 星期日 20:55:41 CST Identify MITE candidates from scratch. 2019年 08月 26日 星期一 00:00:38 CST Finish finding MITE candidates. 2019年 08月 26日 星期一 00:00:38 CST Start to find Helitron candidates. 2019年 08月 26日 星期一 00:00:38 CST Identify Helitron candidates from scratch. 2019年 08月 26日 星期一 02:04:48 CST Finish finding Helitron candidates. 2019年 08月 26日 星期一 02:04:48 CST Execution of EDTA_raw.pl is finished! ################################################################# |
After changing blast version from 2.2.26 to 2.2.22, the |
Seems we have a problem for numpy in our cluster. I changed to another server and now I can fully run EDTA :). Thanks Shujun. |
I will put the requirement for |
Shujun,
Thank you for updating EDTA. I am using 1.3 on a maize genome and the MITE step took a long time (~11 days). The problem is that no MITE raw sequences were output after TIR and MITE runs. Now the running is at Helitron. I will update after the run is finished.
-Sanzhen
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