You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi, I really like percolator and use it in my pipeline with MSGF+.
I'm running a metaproteomic study and hence have a large fasta database (5Gb), I split it to five 1Gb files and ran MSGF+ with each, with -tda 0 (no decoy search) and additionalFeatures on. I generated a decoy database using decoyPyrat and split it too to 5 files and ran MSGF+ against it.
I then merged the .mzid files using the MzidMerger program and performed several runs (different parameters) to convert using msgf2pin.
Each time I get the same error -
double free or corruption (out)
Aborted (core dumped)
I even tried running msgf2pin on one of the split file sets and got the same error.
Anyways, msgf2pin does generate a .tab file of 75.7 Mb that can be fed into percolator, but I guess it is not complete, right?
I would greatly appreciate any advice and help.
Thanks,
Lior
The text was updated successfully, but these errors were encountered:
This sounds like a problem in the format of the mzid file after MzidMerger.
Did you manage to solve the problem already?
If not, please share a (small) example file that causes this problem.
Hi, I really like percolator and use it in my pipeline with MSGF+.
I'm running a metaproteomic study and hence have a large fasta database (5Gb), I split it to five 1Gb files and ran MSGF+ with each, with -tda 0 (no decoy search) and additionalFeatures on. I generated a decoy database using decoyPyrat and split it too to 5 files and ran MSGF+ against it.
I then merged the .mzid files using the MzidMerger program and performed several runs (different parameters) to convert using msgf2pin.
Each time I get the same error -
double free or corruption (out)
Aborted (core dumped)
I even tried running msgf2pin on one of the split file sets and got the same error.
Anyways, msgf2pin does generate a .tab file of 75.7 Mb that can be fed into percolator, but I guess it is not complete, right?
I would greatly appreciate any advice and help.
Thanks,
Lior
The text was updated successfully, but these errors were encountered: