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Bioinformatics for Landscape Genomics

Exercises for workshop organized by year, taught at UNAM-Morelia, Mexico unless otherwise noted in the folder name. Topics include Linux basics, Illumina data quality assessment, SNP calling with Stacks de novo pipeline, and analyzing data with landscape analyses such as GDM, GF, LFMM, or other approaches. I recommend using the most recent year as it reflects the latest versions of software.

Required software

On local computer

Windows: PuTTY and FTP client (e.g., FileZilla or WinSCP)

MacOs: FTP client (e.g., FileZilla)

On server

Stacks

bowtie2

samtools

fastq_screen

fastqc (see "Requirements" section for dependencies)

plink (stable version)

vcftools

admixture

R (optionally with RStudio)

Then in R, run the following commands to install necessary packages (and any dependencies):

install.packages(c("raster", "rgdal", "LEA", "vegan", "gdm"))
install.packages("gradientForest", repos="http://R-Forge.R-project.org")
install.packages("extendedForest", repos="http://R-Forge.R-project.org")
if (!requireNamespace("BiocManager"))
  install.packages("BiocManager")
BiocManager::install()
BiocManager::install(c("qvalue","DESeq2","WGCNA"))