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Position and Cluster_Position #30
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Hi @dsantesmasses, the
Note that
However, we suggest to use CRISPRme does not report the targets' end coordinates, but they can be computed by adding the length of the complete sequence Let us know if you have any further question. Manuel |
Hi @ManuelTgn , thanks very much for your reply! Just to make sure I got it right, in the case of Cas9,
Thanks! |
Hello @dsantesmasses Position corresponds always to the position of the first nucleotide in 5'. NNNNNNNPAM For both - and + strand, since the software applies reverse complement to negative stranded targets. If you use an upstream PAM as cas12, you will have something like this, PAMNNNNNNN Since in this case, the software applies reverse complement to positive stranded targets. Hope this helps and if you have any other question don't hesitate to ask. Best, |
Hi!
Thanks for the quick reply on the other issues! I managed to run it successfully on chr22.
I am inspecting
output.bestMerge.txt
. What doesPosition
correspond to? and what is the difference withCluster_Position
?How can I get start and end coordinates of the sequence in
DNA
?Thanks!
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