hisat2-stringtie_wf_pe.cwl

cwlVersion: v1.0
class: Workflow

inputs:
  ## Common input
  nthreads: int

  ## Inputs for download-sra
  repo: string?
  run_ids: string[]

  ## Inputs for hisat2_mapping
  hisat2_idx_basedir: Directory
  hisat2_idx_basename: string

  ## Inputs for stringtie
  annotation: File

outputs:
  assemble_output:
    type: File
    outputSource: stringtie_assemble/assemble_output

steps:
  download-sra:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/download-sra/download-sra.cwl
    in:
      repo: repo
      run_ids: run_ids
    out:
      [sraFiles]

  pfastq-dump:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/pfastq-dump/pfastq-dump.cwl
    in:
      sraFiles: download-sra/sraFiles
      nthreads: nthreads
    out:
      [forward, reverse]

  hisat2_mapping:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/hisat2/mapping/paired_end/hisat2_mapping_pe.cwl
    in:
      hisat2_idx_basedir: hisat2_idx_basedir
      hisat2_idx_basename: hisat2_idx_basename
      fq1: pfastq-dump/forward
      fq2: pfastq-dump/reverse
      nthreads: nthreads
    out:
      [hisat2_sam]

  samtools_sam2bam:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/samtools/sam2bam/samtools_sam2bam.cwl
    in:
      input_sam: hisat2_mapping/hisat2_sam
    out: [bamfile]

  samtools_sort:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/samtools/sort/samtools_sort.cwl
    in:
      input_bam: samtools_sam2bam/bamfile
      nthreads: nthreads
    out: [sorted_bamfile]

  stringtie_assemble:
    run: https://raw.githubusercontent.com/pitagora-network/pitagora-cwl/master/tools/stringtie/assemble/stringtie_assemble.cwl
    in:
      input_bam: samtools_sort/sorted_bamfile
      nthreads: nthreads
      annotation: annotation
    out: [assemble_output]

$namespaces:
  s: https://schema.org/
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s:license: https://spdx.org/licenses/Apache-2.0
s:codeRepository: https://github.com/pitagora-network/pitagora-cwl
s:author:
  - class: s:Person
    s:identifier: https://orcid.org/0000-0003-3777-5945
    s:email: mailto:inutano@gmail.com
    s:name: Tazro Ohta

$schemas:
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