Running :command:`evol search -h` displays:
usage: evol search [-h] [--quiet] [--examples] [-b] [-s] [-g] [-e FLOAT] [-t INT] [-o STR] [-d STR] query positional arguments: query protein UniProt ID or sequence, a PDB identifier, or a sequence file, where sequence have no gaps and 12 or more characters optional arguments: -h, --help show this help message and exit --quiet suppress info messages to stderr --examples show usage examples and exit sequence search options: -b, --searchBs search Pfam-B families -s, --skipAs do not search Pfam-A families -g, --ga use gathering threshold -e FLOAT, --evalue FLOAT e-value cutoff, must be less than 10.0 -t INT, --timeout INT timeout in seconds for blocking connection attempt (default: 60) output options: -o STR, --outname STR name for output file, default is standard output -d STR, --delimiter STR delimiter for output data columns (default: )
Running :command:`evol search --examples` displays:
Sequence coevolution analysis involves several steps that including retrieving data and refining it for calculations. These steps are illustrated below for RnaseA protein family. Search Pfam database: $ evol search 2w5i Download Pfam MSA file: $ evol fetch RnaseA Refine MSA file: $ evol refine RnaseA_full.slx -l RNAS1_BOVIN --seqid 0.98 --rowocc 0.8 Checking occupancy: $ evol occupancy RnaseA_full.slx -l RNAS1_BOVIN -o col -S Conservation analysis: $ evol conserv RnaseA_full_refined.slx Coevolution analysis: $ evol coevol RnaseA_full_refined.slx -S -c apc Rank order analysis: $ evol rankorder RnaseA_full_refined_mutinfo_corr_apc.txt -p 2w5i_1-121.pdb --seq-sep 3