Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Cell detection only partially supports fluorescence images #14

Closed
petebankhead opened this issue Nov 6, 2016 · 1 comment
Closed

Cell detection only partially supports fluorescence images #14

petebankhead opened this issue Nov 6, 2016 · 1 comment
Assignees
Milestone

Comments

@petebankhead
Copy link
Member

The primary cell detection command under Analyze → Cell analysis → Cell detection is primarily intended for brightfield images.

However, it ought also to work for fluorescence. Under some conditions it does, namely when:

  • the first fluorescence channel contains a nuclear staining (e.g. DAPI)
  • the Max background intensity parameter is either set negative or very high, or Background radius is negative
  • the Threshold value is set appropriately high

This is because, in the case of fluorescence data, the command only looks for the first available channel within which to detect nuclei. Furthermore, the Max background intensity and Threshold parameters, by default, are selected for optical density units... which can be far away from being sensible values for fluorescence.

The command should be adapted to use more sensible defaults in the case of fluorescence images, and to permit the selection of alternative channels.

@petebankhead petebankhead added this to the v0.1 milestone Nov 6, 2016
@petebankhead petebankhead self-assigned this Nov 6, 2016
@petebankhead
Copy link
Member Author

This will be addressed in v0.0.5

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Projects
None yet
Development

No branches or pull requests

1 participant