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error from signalConditions #73
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Thank your for the bug report! And thanks for including the data and the traceback. Could you try the current version of sctransform in the develop branch?
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still not working... attached is the traceback and session info. it looks the same as previously except attached "sctransform" was development version now. By the way, I think it is about the update made in the newest version compared with version 0.3, which running well. |
You are setting I would argue that genes that are detected in just one cell are not going to have a large influence on the downstream analysis and can safely be dropped. In your case, setting The default value for |
Hi there,
"bugmtx.rds" containing the matrix "bugmtx" and the txt file that prints the "err trackback" have been attached in the zip file,and
S <- CreateSeuratObject(counts = bugmtx, min.features = 1,min.cells = 1)
S<- subset(x = S, subset = nFeature_RNA > 200)
S<- SCTransform(S, verbose = T,return.only.var.genes=F, min_cells = 1)
gives err message in console:
Calculating cell attributes from input UMI matrix: log_umi
Variance stabilizing transformation of count matrix of size 11743 by 255
Model formula is y ~ log_umi
Get Negative Binomial regression parameters per gene
Using 2000 genes, 255 cells
|===================== | 25%
warning: solve(): system seems singular; attempting approx solution
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'x' in selecting a method for function 't': missing value where TRUE/FALSE needed
In addition: There were 50 or more warnings (use warnings() to see the first 50)
Called from: signalConditions(obj, exclude = getOption("future.relay.immediate",
"immediateCondition"), resignal = resignal, ...)
session info:
hope to hear your advice soon
thanks
data file and err traceback.zip
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