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spliced and unspliced counts in loom for RNA velocity #42

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Sa753 opened this issue May 30, 2020 · 1 comment
Closed

spliced and unspliced counts in loom for RNA velocity #42

Sa753 opened this issue May 30, 2020 · 1 comment

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@Sa753
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Sa753 commented May 30, 2020

I am trying to use RNAvelocity. I am not sure that I started correctly.

I converted Seurat object to loom using as.loom function but the loom object that is produced doesn't have spliced and unspliced assays needed for RunVelocity function.

Could you please help?
@jmrussell
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You need to run Velocyto on your sample first

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@jmrussell @mojaveazure @Sa753