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this issue is replicated with the latest code at ff93a8b
(lefse1.1.2) $ lefse_format_input.py LEfSe.txt input.in -c 1 -o 1000000 (lefse1.1.2) $ lefse_run.py input.in input.res Number of significantly discriminative features: 11 ( 115 ) before internal wilcoxon Number of discriminative features with abs LDA score > 2.0 : 11 (lefse1.1.2) $ lefse_plot_res.py input.res input.png (lefse1.1.2) $ lefse_plot_cladogram.py input.res input.cladogram.png --format png clade_sep parameter too large, lowered to 0.15016937255859375 (lefse1.1.2) $ lefse_plot_features.py input.in input.res biomarkers_raw_images/ Exporting Bacteria.Acidobacteria /home/agean/anaconda3/envs/lefse1.1.2/lib/python3.10/site-packages/lefse/lefse_plot_features.py:99: UserWarning: color is redundantly defined by the 'color' keyword argument and the fmt string "k--" (-> color='k'). The keyword argument will take precedence. if params['subcl_median'] == 'y': ax.plot([fr,to-1],[median,median],"k--",linewidth=1,color=params['fore_color']) Exporting Bacteria.Acidobacteria.Acidobacteria Exporting Bacteria.Proteobacteria.Betaproteobacteria Exporting Bacteria.Acidobacteria.Acidobacteria.Subgroup_4 Exporting Bacteria.Acidobacteria.Acidobacteria.Subgroup_4.Unknown_Family Exporting Bacteria.Firmicutes.Clostridia.Clostridiales.Clostridiaceae_1 Exporting Bacteria.Acidobacteria.Acidobacteria.Subgroup_4.Unknown_Family.Blastocatella Exporting Bacteria.Firmicutes.Clostridia.Clostridiales.Clostridiaceae_1.Clostridium_sensu_stricto_1 Exporting Bacteria.Firmicutes.Clostridia.Clostridiales.Clostridiaceae_1.Clostridium_sensu_stricto_5 Exporting Bacteria.Proteobacteria.Gammaproteobacteria.Xanthomonadales.Xanthomonadaceae.Lysobacter Exporting Bacteria.Proteobacteria.Gammaproteobacteria.Xanthomonadales.Xanthomonadaceae.Thermomonas
and the header of the input.txt:
Class BS BS BS BS BS BS BS BS BS BS BS BS BS BS BS BS BS BS B B B B B B B B B B B B B B B B B B CK CK CK CK CK CK CK CK CK CK CK CK CK CK CK CK CK CK GB GB GB GB GB GB GB GB GB GB GB GB GB GB GB GB GB GB G G G G G G G G G G G G G G G G G G S S S S S S S S S S S S S S S S S Bacteria 30.6630944407234 28.0821189235188 26.2622912439519 28.464878565641 34.0280283892698 24.8668178701408 22.8306348917591 31.542049846728 23.2917247712663 31.2347608627696 29.4716136471546 33.8675415407855 23.7967782722212 20.9295630477896 16.8389490772952 41.5306788511749 27.9520872441226 41.5160760384886 32.0162510953557 30.5940213210243 34.4588532746106 27.2795123771613 30.7127927313057 30.4716322664274 32.4128984432913 34.3659315147998 34.1582780535325 26.6592442798391 7.51135216952573 27.68968456948 26.8868323548818 27.1602061915718 33.0120737131964 31.2339943342776 27.3729269730603 30.3363761410529 28.8902074636038 28.4102670254606 39.7076572348129 32.3783972938295 29.5300601623964 33.4062458821979 33.1469209633102 35.9502682107585 36.2998335865712 24.3506352716708 23.7893388631373 23.256418251741 28.0696576151122 21.6789806996381 23.8571794215511 36.2356581152507 35.8637953914827 37.3437666915928 32.8514798366666 34.4840999708256 35.1296847853065 72.7115748820974 71.1305790044441 74.8551649646504 43.1020881670534 37.8530510585305 39.7420472592521 39.573396655135 13.5892681862799 40.7100192068216 43.8096802677783 34.1613101198359 25.9357010744197 61.0303454919648 65.8615798885897 64.4623346751006 37.0374356490699 39.2138401788867 33.2591449977964 72.4046368770435 72.6245608431811 74.7023809523809 37.3352244284999 41.5997726788378 44.1844532279315 26.6893642217452 27.5398071316439 32.6823014855592 28.6704100556357 28.2418676698385 21.1531816162314 66.9901899394698 69.2487698195735 60.8118064703387 31.4522599625568 31.8027103021078 24.6580792758741 23.507164544825 26.4721392160309 20.0196976149915 29.3584854263144 29.0867374695461 36.7328458560659 36.2979081847536 29.657192887931 21.7576749491643 25.3858360818968 25.2721021785988 37.7703724904935 44.7464562326465 39.7644054731162 Bacteria|Acidobacteria 0.116097343156955 0.0936070630783959 0.0916965818635867 0.0519394178630046 0.0390953927583303 0.0131863494910069 0.0254684373294524 0.0666400106624017 0.0748273868235774 0.112535167239762 0.0768726401229962 0.0849697885196375 0.206899949096044 0.13274902437464 0.115959314846105 0.0550750652741514 0.0774708739310509 0.058671673316123 0.220398842242226 0.175843499285636 0.280020661718729 0.0787826014844301 0.0575864863711982 0.0598265031408914 0.240919199406968 0.340975043528729 0.243540381314654 0.181772738633523 0.0567608476286579 0.381926683716965 0.504170402828768 0.372775722981041 0.431582291887312 0.0634560906515581 0.128162407402661 0.112542203326247 0.233105172815699 0.0672738563444421 0.301729918197667 0.08865041409075 0.132525558500568 0.0658848333113717 0.265071925004041 0.308317040156414 0.465475242987724 0.28402931355969 0.143327640888021 0.223469493815612 0.346141461843941 0.282720144752714 0.258510366009726 0.208843625112801 0.0965134515623115 0.0293772032902468 0.05066913057733 0.0486239424292522 0.0620501365103003 0.024137546882543 0.00850539029109698 0.0196386488609584 0.0603248259860789 0.0473225404732254 0.0794070937003706 0.0941638861026386 0.0581670120333006 0.160408619852677 0.112943302462164 0.0952873154817662 0.0768927035058916 0.0262731532162718 0.0308331106497564 0.0123223527478847 0.0627813951819764 0.0294221489937625 0.143234905244601 0.00990785693054592 0.00598850207601405 0.00220458553791887 0.0389343122531843 0.0449906466813478 0.0395256916996047 0.159129598105248 0.156985871271586 0.17595455345248 0.293632804450855 0.154901526886479 0.182652910105149 0.0438321853475266 0.0262438490978677 0.0379557480631405 0.130382455201926 0.103591890234879 0.172410934831982 0.0665849890800618 0.0615222358938302 0.0851788756388416 0.146417869790248 0.0898833881306621 0.162995494985625 0.0795544948289578 0.148168103448276 0.175238473110814 0.161802227892215 0.288579724602312 0.122664267898761 0.0730673681134006 0.0674503757949509 Bacteria|Actinobacteria 0.82830989060058 1.02329539410701 1.00085843608553 1.69322502233395 1.21496451341273 2.93264412679994 2.139348735674 1.25283220045315 1.65980748954117 0.803376055017193 1.14404576183047 1.13765105740181 0.821031544031922 0.799692917919519 0.977371367988603 1.56453981723238 1.38553678376687 1.50903543769068 1.30114979155049 1.79415320364875 1.21795394611641 4.48646183190281 2.86652732158853 2.84923721208495 1.48026315789474 1.69762042948346 1.5470612793988 1.80409443093772 0.113521695257316 2.13128729752771 0.795168084998393 0.765937618312607 0.680470239356069 1.88101983002833 2.46584471842719 2.32274602976116 0.436542414545763 0.507141378596564 1.0694649322784 4.05458999183483 3.22058142959317 3.50243773883252 1.20575400032326 1.43630621146037 1.2468948218894 1.22284376219592 1.69553549646255 1.58767279908533 1.0759901255769 1.34574788902292 1.27975428717686 1.08031455459585 1.57739172397153 2.28340989210554 1.16240946618581 0.717203150831469 1.38682055100521 0.271083218834713 0.433774904845946 0.375589159465829 1.42227378190255 1.27272727272727 1.7204870301747 1.40427012753065 0.737993965172502 1.01943899195846 1.10479084953899 0.702542071772344 0.442652590452836 0.823225467443184 0.628995457255031 0.751663517620965 0.89867082817629 1.23278804283865 1.01366240634641 0.34083027841078 0.461114659853082 0.440917107583774 1.07625563157017 1.00636972839857 1.45718050065876 2.30182814003405 1.70441803094864 1.45790915717769 1.79785699567278 0.146603230803275 1.37730167349558 0.540596952619495 0.376161837069437 0.875214896514769 0.775608451457609 0.471448806579142 0.606753866812553 2.35977201299739 2.55097556688346 1.03545570698467 1.00789975483519 1.39319251602526 0.830824259162837 0.807244138705602 0.737473060344828 0.704260278728364 0.847905905781318 0.575378092879919 1.3758842049311 1.12036631107214 1.75370977066872
the output res and cladogram only show 4 groups:
the feature plot is ok.
The text was updated successfully, but these errors were encountered:
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this issue is replicated with the latest code at ff93a8b
and the header of the input.txt:
the output res and cladogram only show 4 groups:
![input](https://user-images.githubusercontent.com/4170246/173543763-aff75a10-23c2-4a9d-9a37-7afd9413c692.png)
![input cladogram](https://user-images.githubusercontent.com/4170246/173543789-b5ff81e2-92b8-4604-ac48-689ea798aac9.png)
the feature plot is ok.
![1_Bacteria-Acidobacteria-Acidobacteria-Subgroup_4-Unknown_Family](https://user-images.githubusercontent.com/4170246/173543869-9d783d03-40a9-483f-ae04-8e13be767bf6.png)
The text was updated successfully, but these errors were encountered: