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MarBTNgenome

Code to accompany publication on genome evolution of soft-shell clam transmissible cancer

Samuel Hart, University of Washington Molecular and Cellular Biology PhD program

Michael Metzger, Pacific Northwest Research Institute



If you use any of these methods or data for your own research, please use the following citation:



Raw sequence data and the assembled genome are available via NCBI BioProject PRJNA874712

Software installations used:

  • bedtools (2.29.1)
  • BBTools (38.86)
  • trimmomatic (0.36)
  • bwa (0.7.12)
  • samtools (1.9)
  • somatypus (1.3)
  • helmsman (1.5.2)
  • bcftools (1.10.2)
  • delly (0.8.5)
  • seqtk (1.0)
  • blast+ (2.10.0)
  • CD-HIT (4.8.1)
  • RepeatModeler (2.0)
  • Repeatmasker (4.1.0)
  • REPdenovo (2019.07.20 download)
  • supernova (2.1.1)
  • FALCON-Unzip (with pbbioconda-0.0.5 and python 3.7)
  • FALCON-Phase (v0.1.0-beta)
  • SNAP(version 2006-07-28)
  • PBSuite (15.8.24, slightly modified: https://github.com/esrice/PBJelly)
  • blasr (5.1)
  • networkx (2.2 with Python 2.7)
  • Longranger62 (2.2.2)
  • FreeBayes (1.3.1)
  • Trinity (2.8.5)
  • MAKER (2.31.10)
  • exonerate (2.2.0)
  • BUSCO (v3)
  • telseq (v0.0.2)
  • STAR (2.7.5a_2020-06-29)

R packages used (R v3.6.0)

  • Biostrings (2.54.0)
  • sigfit (2.0.0)
  • mapdata (2.3.0)
  • maptools (1.1-1)
  • tidyverse (1.3.0)
  • ape (5.5)
  • lsa (0.73.2)
  • gridExtra (2.3)
  • zoo (1.8-8)
  • geiger (2.0.7)
  • nlme (3.1-139)
  • phytools (0.7-90)
  • dndscv (0.0.1.0)
  • devtools (2.3.2)
  • cn.mops (1.32.0)
  • mixtools (1.2.0)
  • bedr (1.0.7)
  • ggseqlogo (0.1)
  • DESeq2 (1.26.0)
  • pheatmap (1.0.12)
  • RColorBrewer (1.1.3)
  • viridis (0.5.1)
  • scales (1.2.1)

Note that paths were designed for our institutes working environment: paths will need to be altered manually to reproduce this analysis