doc/sourmash-collections.ipynb

{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# sourmash: working with private collections of signatures\n",
    "\n",
    "### Running this notebook.\n",
    "\n",
    "You can run this notebook interactively via mybinder; click on this button:\n",
    "[![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/dib-lab/sourmash/master?filepath=doc%2Fsourmash-collections.ipynb)\n",
    "\n",
    "A rendered version of this notebook is available at [sourmash.readthedocs.io](https://sourmash.readthedocs.io) under \"Tutorials and notebooks\".\n",
    "\n",
    "You can also get this notebook from the [doc/ subdirectory of the sourmash github repository](https://github.com/dib-lab/sourmash/tree/master/doc). See [binder/environment.yaml](https://github.com/dib-lab/sourmash/blob/master/binder/environment.yml) for installation dependencies.\n",
    "\n",
    "### What is this?\n",
    "\n",
    "This is a Jupyter Notebook using Python 3. If you are running this via [binder](https://mybinder.org), you can use Shift-ENTER to run cells, and double click on code cells to edit them.\n",
    "\n",
    "Contact: C. Titus Brown, ctbrown@ucdavis.edu. Please [file issues on GitHub](https://github.com/dib-lab/sourmash/issues/) if you have any questions or comments!"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## download a bunch of genomes"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "/Users/t/dev/sourmash/doc/big_genomes\n",
      "  % Total    % Received % Xferd  Average Speed   Time    Time     Time  Current\n",
      "                                 Dload  Upload   Total   Spent    Left  Speed\n",
      "100   459  100   459    0     0    750      0 --:--:-- --:--:-- --:--:--   750\n",
      "100 61.1M  100 61.1M    0     0  2966k      0  0:00:21  0:00:21 --:--:-- 3496k\n"
     ]
    }
   ],
   "source": [
    "!mkdir -p big_genomes\n",
    "!curl -L https://osf.io/8uxj9/?action=download | (cd big_genomes && tar xzf -)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## compute signatures for each file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "/Users/t/dev/sourmash/doc/big_genomes\n",
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Ksetting num_hashes to 0 because --scaled is set\n",
      "\u001b[Kcomputing signatures for files: 0.fa, 1.fa, 10.fa, 11.fa, 12.fa, 13.fa, 14.fa, 15.fa, 16.fa, 17.fa, 18.fa, 19.fa, 2.fa, 20.fa, 21.fa, 22.fa, 23.fa, 24.fa, 25.fa, 26.fa, 27.fa, 28.fa, 29.fa, 3.fa, 30.fa, 31.fa, 32.fa, 33.fa, 34.fa, 35.fa, 36.fa, 37.fa, 38.fa, 39.fa, 4.fa, 40.fa, 41.fa, 42.fa, 43.fa, 44.fa, 45.fa, 46.fa, 47.fa, 48.fa, 49.fa, 5.fa, 50.fa, 51.fa, 52.fa, 53.fa, 54.fa, 55.fa, 56.fa, 57.fa, 58.fa, 59.fa, 6.fa, 60.fa, 61.fa, 62.fa, 63.fa, 7.fa, 8.fa, 9.fa\n",
      "\u001b[KComputing signature for ksizes: [31]\n",
      "\u001b[KComputing only nucleotide (and not protein) signatures.\n",
      "\u001b[KComputing a total of 1 signature(s).\n",
      "\u001b[K... reading sequences from 0.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 0.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 1.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 1.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 10.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 10.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 11.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 11.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 12.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 12.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 13.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 13.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 14.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 14.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 15.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 15.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 16.fa\n",
      "\u001b[Kcalculated 1 signatures for 4 sequences in 16.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 17.fa\n",
      "\u001b[Kcalculated 1 signatures for 2 sequences in 17.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 18.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 18.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 19.fa\n",
      "\u001b[Kcalculated 1 signatures for 9 sequences in 19.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 2.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 2.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 20.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 20.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 21.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 21.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 22.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 22.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 23.fa\n",
      "\u001b[Kcalculated 1 signatures for 5 sequences in 23.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 24.fa\n",
      "\u001b[Kcalculated 1 signatures for 3 sequences in 24.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 25.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 25.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 26.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 26.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 27.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 27.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 28.fa\n",
      "\u001b[Kcalculated 1 signatures for 3 sequences in 28.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 29.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 29.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 3.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 3.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 30.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 30.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 31.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 31.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 32.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 32.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 33.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 33.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 34.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 34.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 35.fa\n",
      "\u001b[Kcalculated 1 signatures for 7 sequences in 35.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 36.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 36.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 37.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 37.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 38.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 38.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 39.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 39.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 4.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 4.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 40.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 40.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 41.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 41.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 42.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 42.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 43.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 43.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 44.fa\n",
      "\u001b[Kcalculated 1 signatures for 2 sequences in 44.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 45.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 45.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 46.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 46.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 47.fa\n",
      "\u001b[Kcalculated 1 signatures for 2 sequences in 47.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 48.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 48.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 49.fa\n",
      "\u001b[Kcalculated 1 signatures for 228 sequences in 49.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 5.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 5.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 50.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 50.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 51.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 51.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 52.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 52.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 53.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 53.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 54.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 54.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 55.fa\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[Kcalculated 1 signatures for 1 sequences in 55.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 56.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 56.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 57.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 57.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 58.fa\n",
      "\u001b[Kcalculated 1 signatures for 30 sequences in 58.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 59.fa\n",
      "\u001b[Kcalculated 1 signatures for 5 sequences in 59.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 6.fa\n",
      "\u001b[Kcalculated 1 signatures for 76 sequences in 6.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 60.fa\n",
      "\u001b[Kcalculated 1 signatures for 11 sequences in 60.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 61.fa\n",
      "\u001b[Kcalculated 1 signatures for 47 sequences in 61.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 62.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 62.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 63.fa\n",
      "\u001b[Kcalculated 1 signatures for 4 sequences in 63.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 7.fa\n",
      "\u001b[Kcalculated 1 signatures for 3 sequences in 7.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 8.fa\n",
      "\u001b[Kcalculated 1 signatures for 1 sequences in 8.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n",
      "\u001b[K... reading sequences from 9.fa\n",
      "\u001b[Kcalculated 1 signatures for 3 sequences in 9.fa\n",
      "\u001b[Ksaved 1 signature(s). Note: signature license is CC0.\n"
     ]
    }
   ],
   "source": [
    "!cd big_genomes/ && sourmash compute -k 31 --scaled=1000 --name-from-first *.fa"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Compare them all"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kloaded 64 signatures total.                                                    \n",
      "\u001b[Kdownsampling to scaled value of 1000\n",
      "\u001b[K\n",
      "min similarity in matrix: 0.000\n",
      "\u001b[Ksaving labels to: compare_all.mat.labels.txt\n",
      "\u001b[Ksaving distance matrix to: compare_all.mat\n",
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kloading comparison matrix from compare_all.mat...\n",
      "\u001b[K...got 64 x 64 matrix.\n",
      "\u001b[Kloading labels from compare_all.mat.labels.txt\n",
      "\u001b[Ksaving histogram of matrix values => compare_all.mat.hist.png\n",
      "\u001b[Kwrote dendrogram to: compare_all.mat.dendro.png\n",
      "\u001b[Kwrote numpy distance matrix to: compare_all.mat.matrix.png\n"
     ]
    }
   ],
   "source": [
    "!sourmash compare big_genomes/*.sig -o compare_all.mat\n",
    "!sourmash plot compare_all.mat"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "image/png": 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\n",
      "text/plain": [
       "<IPython.core.display.Image object>"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "from IPython.display import Image\n",
    "Image(filename='compare_all.mat.matrix.png') "
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## make a fast(er) search database for all of them"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kloading 64 files into SBT\n",
      "\u001b[Kreading from big_genomes/9.fa.sig (63 signatures so far))\n",
      "\u001b[Kloaded 64 sigs; saving SBT under \"all-genomes\"\n",
      "\u001b[K127 of 127 nodes saved\n",
      "Finished saving nodes, now saving SBT json file.\n"
     ]
    }
   ],
   "source": [
    "!sourmash index -k 31 all-genomes big_genomes/*.sig"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "You can now use this to search, and gather."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kselecting default query k=31.\n",
      "\u001b[Kloaded query: NC_009665.1 Shewanella baltica... (k=31, DNA)\n",
      "\u001b[Kloaded 1 databases.                                                            \n",
      "\n",
      "2 matches:\n",
      "similarity   match\n",
      "----------   -----\n",
      "  9.5%       NC_009665.1 Shewanella baltica OS185, complete genome\n",
      "  4.4%       NC_011663.1 Shewanella baltica OS223, complete genome\n"
     ]
    }
   ],
   "source": [
    "!sourmash search shew_os185.fa.sig all-genomes --threshold=0.001"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\r",
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\r\n",
      "\r",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\r\n",
      "\r\n",
      "\r",
      "\u001b[Ksetting num_hashes to 0 because --scaled is set\r\n",
      "\r",
      "\u001b[Kcomputing signatures for files: fake-metagenome.fa\r\n",
      "\r",
      "\u001b[KComputing signature for ksizes: [31]\r\n",
      "\r",
      "\u001b[KComputing only nucleotide (and not protein) signatures.\r\n",
      "\r",
      "\u001b[KComputing a total of 1 signature(s).\r\n",
      "\r",
      "\u001b[Kskipping fake-metagenome.fa - already done\r\n"
     ]
    }
   ],
   "source": [
    "# (make fake metagenome again, just in case)\n",
    "!cat genomes/*.fa > fake-metagenome.fa\n",
    "!sourmash compute -k 31 --scaled=1000 fake-metagenome.fa"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kselect query k=31 automatically.\n",
      "\u001b[Kloaded query: fake-metagenome.fa... (k=31, DNA)\n",
      "\u001b[Kloaded 1 databases.                                                            \n",
      "\n",
      "\n",
      "overlap     p_query p_match\n",
      "---------   ------- -------\n",
      "0.5 Mbp       42.2%   10.5%    NC_011663.1 Shewanella baltica OS223,...\n",
      "499.0 kbp     38.4%   18.5%    CP001071.1 Akkermansia muciniphila AT...\n",
      "0.5 Mbp       19.4%    4.9%    NC_009665.1 Shewanella baltica OS185,...\n",
      "\n",
      "found 3 matches total;\n",
      "the recovered matches hit 100.0% of the query\n",
      "\n"
     ]
    }
   ],
   "source": [
    "!sourmash gather fake-metagenome.fa.sig all-genomes"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# build a database with taxonomic information --\n",
    "\n",
    "for this, we need to provide a metadata file that contains accession => tax information."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>accession</th>\n",
       "      <th>taxid</th>\n",
       "      <th>superkingdom</th>\n",
       "      <th>phylum</th>\n",
       "      <th>class</th>\n",
       "      <th>order</th>\n",
       "      <th>family</th>\n",
       "      <th>genus</th>\n",
       "      <th>species</th>\n",
       "      <th>strain</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>AE000782</td>\n",
       "      <td>224325</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Archaeoglobi</td>\n",
       "      <td>Archaeoglobales</td>\n",
       "      <td>Archaeoglobaceae</td>\n",
       "      <td>Archaeoglobus</td>\n",
       "      <td>Archaeoglobus fulgidus</td>\n",
       "      <td>Archaeoglobus fulgidus DSM 4304</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>NC_000909</td>\n",
       "      <td>243232</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Methanococci</td>\n",
       "      <td>Methanococcales</td>\n",
       "      <td>Methanocaldococcaceae</td>\n",
       "      <td>Methanocaldococcus</td>\n",
       "      <td>Methanocaldococcus jannaschii</td>\n",
       "      <td>Methanocaldococcus jannaschii DSM 2661</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>NC_003272</td>\n",
       "      <td>103690</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Cyanobacteria</td>\n",
       "      <td>NaN</td>\n",
       "      <td>Nostocales</td>\n",
       "      <td>Nostocaceae</td>\n",
       "      <td>Nostoc</td>\n",
       "      <td>Nostoc sp. PCC 7120</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>AE009441</td>\n",
       "      <td>178306</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Crenarchaeota</td>\n",
       "      <td>Thermoprotei</td>\n",
       "      <td>Thermoproteales</td>\n",
       "      <td>Thermoproteaceae</td>\n",
       "      <td>Pyrobaculum</td>\n",
       "      <td>Pyrobaculum aerophilum</td>\n",
       "      <td>Pyrobaculum aerophilum str. IM2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>AE009950</td>\n",
       "      <td>186497</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Thermococci</td>\n",
       "      <td>Thermococcales</td>\n",
       "      <td>Thermococcaceae</td>\n",
       "      <td>Pyrococcus</td>\n",
       "      <td>Pyrococcus furiosus</td>\n",
       "      <td>Pyrococcus furiosus DSM 3638</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>AE009951</td>\n",
       "      <td>190304</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Fusobacteria</td>\n",
       "      <td>Fusobacteriia</td>\n",
       "      <td>Fusobacteriales</td>\n",
       "      <td>Fusobacteriaceae</td>\n",
       "      <td>Fusobacterium</td>\n",
       "      <td>Fusobacterium nucleatum</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>AE010299</td>\n",
       "      <td>188937</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Methanomicrobia</td>\n",
       "      <td>Methanosarcinales</td>\n",
       "      <td>Methanosarcinaceae</td>\n",
       "      <td>Methanosarcina</td>\n",
       "      <td>Methanosarcina acetivorans</td>\n",
       "      <td>Methanosarcina acetivorans C2A</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>AE009439</td>\n",
       "      <td>190192</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Methanopyri</td>\n",
       "      <td>Methanopyrales</td>\n",
       "      <td>Methanopyraceae</td>\n",
       "      <td>Methanopyrus</td>\n",
       "      <td>Methanopyrus kandleri</td>\n",
       "      <td>Methanopyrus kandleri AV19</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>NC_003911</td>\n",
       "      <td>246200</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Alphaproteobacteria</td>\n",
       "      <td>Rhodobacterales</td>\n",
       "      <td>Rhodobacteraceae</td>\n",
       "      <td>Ruegeria</td>\n",
       "      <td>Ruegeria pomeroyi</td>\n",
       "      <td>Ruegeria pomeroyi DSS-3</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>AE006470</td>\n",
       "      <td>194439</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chlorobi</td>\n",
       "      <td>Chlorobia</td>\n",
       "      <td>Chlorobiales</td>\n",
       "      <td>Chlorobiaceae</td>\n",
       "      <td>Chlorobaculum</td>\n",
       "      <td>Chlorobaculum tepidum</td>\n",
       "      <td>Chlorobaculum tepidum TLS</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>AE015928</td>\n",
       "      <td>226186</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Bacteroidetes</td>\n",
       "      <td>Bacteroidia</td>\n",
       "      <td>Bacteroidales</td>\n",
       "      <td>Bacteroidaceae</td>\n",
       "      <td>Bacteroides</td>\n",
       "      <td>Bacteroides thetaiotaomicron</td>\n",
       "      <td>Bacteroides thetaiotaomicron VPI-5482</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>AL954747</td>\n",
       "      <td>228410</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Betaproteobacteria</td>\n",
       "      <td>Nitrosomonadales</td>\n",
       "      <td>Nitrosomonadaceae</td>\n",
       "      <td>Nitrosomonas</td>\n",
       "      <td>Nitrosomonas europaea</td>\n",
       "      <td>Nitrosomonas europaea ATCC 19718</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>BX119912</td>\n",
       "      <td>243090</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Planctomycetes</td>\n",
       "      <td>Planctomycetia</td>\n",
       "      <td>Planctomycetales</td>\n",
       "      <td>Planctomycetaceae</td>\n",
       "      <td>Rhodopirellula</td>\n",
       "      <td>Rhodopirellula baltica</td>\n",
       "      <td>Rhodopirellula baltica SH 1</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>BX571656</td>\n",
       "      <td>273121</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Epsilonproteobacteria</td>\n",
       "      <td>Campylobacterales</td>\n",
       "      <td>Helicobacteraceae</td>\n",
       "      <td>Wolinella</td>\n",
       "      <td>Wolinella succinogenes</td>\n",
       "      <td>Wolinella succinogenes DSM 1740</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>AE017180</td>\n",
       "      <td>243231</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Deltaproteobacteria</td>\n",
       "      <td>Desulfuromonadales</td>\n",
       "      <td>Geobacteraceae</td>\n",
       "      <td>Geobacter</td>\n",
       "      <td>Geobacter sulfurreducens</td>\n",
       "      <td>Geobacter sulfurreducens PCA</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>15</th>\n",
       "      <td>AE017226</td>\n",
       "      <td>243275</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Spirochaetes</td>\n",
       "      <td>Spirochaetia</td>\n",
       "      <td>Spirochaetales</td>\n",
       "      <td>Spirochaetaceae</td>\n",
       "      <td>Treponema</td>\n",
       "      <td>Treponema denticola</td>\n",
       "      <td>Treponema denticola ATCC 35405</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>16</th>\n",
       "      <td>BX950229</td>\n",
       "      <td>267377</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Methanococci</td>\n",
       "      <td>Methanococcales</td>\n",
       "      <td>Methanococcaceae</td>\n",
       "      <td>Methanococcus</td>\n",
       "      <td>Methanococcus maripaludis</td>\n",
       "      <td>Methanococcus maripaludis S2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>AE017221</td>\n",
       "      <td>262724</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Deinococcus-Thermus</td>\n",
       "      <td>Deinococci</td>\n",
       "      <td>Thermales</td>\n",
       "      <td>Thermaceae</td>\n",
       "      <td>Thermus</td>\n",
       "      <td>Thermus thermophilus</td>\n",
       "      <td>Thermus thermophilus HB27</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>18</th>\n",
       "      <td>BA000001</td>\n",
       "      <td>70601</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Thermococci</td>\n",
       "      <td>Thermococcales</td>\n",
       "      <td>Thermococcaceae</td>\n",
       "      <td>Pyrococcus</td>\n",
       "      <td>Pyrococcus horikoshii</td>\n",
       "      <td>Pyrococcus horikoshii OT3</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>BA000023</td>\n",
       "      <td>273063</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Crenarchaeota</td>\n",
       "      <td>Thermoprotei</td>\n",
       "      <td>Sulfolobales</td>\n",
       "      <td>Sulfolobaceae</td>\n",
       "      <td>Sulfolobus</td>\n",
       "      <td>Sulfolobus tokodaii</td>\n",
       "      <td>Sulfolobus tokodaii str. 7</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>NC_007951</td>\n",
       "      <td>266265</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Betaproteobacteria</td>\n",
       "      <td>Burkholderiales</td>\n",
       "      <td>Burkholderiaceae</td>\n",
       "      <td>Paraburkholderia</td>\n",
       "      <td>Paraburkholderia xenovorans</td>\n",
       "      <td>Paraburkholderia xenovorans LB400</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>21</th>\n",
       "      <td>CP000492</td>\n",
       "      <td>290317</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chlorobi</td>\n",
       "      <td>Chlorobia</td>\n",
       "      <td>Chlorobiales</td>\n",
       "      <td>Chlorobiaceae</td>\n",
       "      <td>Chlorobium</td>\n",
       "      <td>Chlorobium phaeobacteroides</td>\n",
       "      <td>Chlorobium phaeobacteroides DSM 266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>22</th>\n",
       "      <td>NC_008751</td>\n",
       "      <td>391774</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Deltaproteobacteria</td>\n",
       "      <td>Desulfovibrionales</td>\n",
       "      <td>Desulfovibrionaceae</td>\n",
       "      <td>Desulfovibrio</td>\n",
       "      <td>Desulfovibrio vulgaris</td>\n",
       "      <td>Desulfovibrio vulgaris DP4</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>23</th>\n",
       "      <td>CP000568</td>\n",
       "      <td>203119</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Firmicutes</td>\n",
       "      <td>Clostridia</td>\n",
       "      <td>Clostridiales</td>\n",
       "      <td>Ruminococcaceae</td>\n",
       "      <td>Ruminiclostridium</td>\n",
       "      <td>Ruminiclostridium thermocellum</td>\n",
       "      <td>Ruminiclostridium thermocellum ATCC 27405</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>24</th>\n",
       "      <td>CP000561</td>\n",
       "      <td>410359</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Crenarchaeota</td>\n",
       "      <td>Thermoprotei</td>\n",
       "      <td>Thermoproteales</td>\n",
       "      <td>Thermoproteaceae</td>\n",
       "      <td>Pyrobaculum</td>\n",
       "      <td>Pyrobaculum calidifontis</td>\n",
       "      <td>Pyrobaculum calidifontis JCM 11548</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>25</th>\n",
       "      <td>CP000609</td>\n",
       "      <td>402880</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Methanococci</td>\n",
       "      <td>Methanococcales</td>\n",
       "      <td>Methanococcaceae</td>\n",
       "      <td>Methanococcus</td>\n",
       "      <td>Methanococcus maripaludis</td>\n",
       "      <td>Methanococcus maripaludis C5</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>26</th>\n",
       "      <td>CP000607</td>\n",
       "      <td>290318</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chlorobi</td>\n",
       "      <td>Chlorobia</td>\n",
       "      <td>Chlorobiales</td>\n",
       "      <td>Chlorobiaceae</td>\n",
       "      <td>Chlorobium</td>\n",
       "      <td>Chlorobium phaeovibrioides</td>\n",
       "      <td>Chlorobium phaeovibrioides DSM 265</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>27</th>\n",
       "      <td>CP000660</td>\n",
       "      <td>340102</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Crenarchaeota</td>\n",
       "      <td>Thermoprotei</td>\n",
       "      <td>Thermoproteales</td>\n",
       "      <td>Thermoproteaceae</td>\n",
       "      <td>Pyrobaculum</td>\n",
       "      <td>Pyrobaculum arsenaticum</td>\n",
       "      <td>Pyrobaculum arsenaticum DSM 13514</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>28</th>\n",
       "      <td>CP000667</td>\n",
       "      <td>369723</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Actinobacteria</td>\n",
       "      <td>Actinobacteria</td>\n",
       "      <td>Micromonosporales</td>\n",
       "      <td>Micromonosporaceae</td>\n",
       "      <td>Salinispora</td>\n",
       "      <td>Salinispora tropica</td>\n",
       "      <td>Salinispora tropica CNB-440</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>29</th>\n",
       "      <td>CP000679</td>\n",
       "      <td>351627</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Firmicutes</td>\n",
       "      <td>Clostridia</td>\n",
       "      <td>Thermoanaerobacterales</td>\n",
       "      <td>Thermoanaerobacterales Family III. Incertae Sedis</td>\n",
       "      <td>Caldicellulosiruptor</td>\n",
       "      <td>Caldicellulosiruptor saccharolyticus</td>\n",
       "      <td>Caldicellulosiruptor saccharolyticus DSM 8903</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>34</th>\n",
       "      <td>CP000850</td>\n",
       "      <td>391037</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Actinobacteria</td>\n",
       "      <td>Actinobacteria</td>\n",
       "      <td>Micromonosporales</td>\n",
       "      <td>Micromonosporaceae</td>\n",
       "      <td>Salinispora</td>\n",
       "      <td>Salinispora arenicola</td>\n",
       "      <td>Salinispora arenicola CNS-205</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>35</th>\n",
       "      <td>CP000909</td>\n",
       "      <td>324602</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chloroflexi</td>\n",
       "      <td>Chloroflexia</td>\n",
       "      <td>Chloroflexales</td>\n",
       "      <td>Chloroflexaceae</td>\n",
       "      <td>Chloroflexus</td>\n",
       "      <td>Chloroflexus aurantiacus</td>\n",
       "      <td>Chloroflexus aurantiacus J-10-fl</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>36</th>\n",
       "      <td>CP000924</td>\n",
       "      <td>340099</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Firmicutes</td>\n",
       "      <td>Clostridia</td>\n",
       "      <td>Thermoanaerobacterales</td>\n",
       "      <td>Thermoanaerobacteraceae</td>\n",
       "      <td>Thermoanaerobacter</td>\n",
       "      <td>Thermoanaerobacter pseudethanolicus</td>\n",
       "      <td>Thermoanaerobacter pseudethanolicus ATCC 33223</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>37</th>\n",
       "      <td>CP000969</td>\n",
       "      <td>126740</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Thermotogae</td>\n",
       "      <td>Thermotogae</td>\n",
       "      <td>Thermotogales</td>\n",
       "      <td>Thermotogaceae</td>\n",
       "      <td>Thermotoga</td>\n",
       "      <td>Thermotoga sp. RQ2</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>38</th>\n",
       "      <td>CP001013</td>\n",
       "      <td>395495</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Betaproteobacteria</td>\n",
       "      <td>Burkholderiales</td>\n",
       "      <td>NaN</td>\n",
       "      <td>Leptothrix</td>\n",
       "      <td>Leptothrix cholodnii</td>\n",
       "      <td>Leptothrix cholodnii SP-6</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>39</th>\n",
       "      <td>CP001071</td>\n",
       "      <td>349741</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Verrucomicrobia</td>\n",
       "      <td>Verrucomicrobiae</td>\n",
       "      <td>Verrucomicrobiales</td>\n",
       "      <td>Akkermansiaceae</td>\n",
       "      <td>Akkermansia</td>\n",
       "      <td>Akkermansia muciniphila</td>\n",
       "      <td>Akkermansia muciniphila ATCC BAA-835</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>40</th>\n",
       "      <td>AP009380</td>\n",
       "      <td>431947</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Bacteroidetes</td>\n",
       "      <td>Bacteroidia</td>\n",
       "      <td>Bacteroidales</td>\n",
       "      <td>Porphyromonadaceae</td>\n",
       "      <td>Porphyromonas</td>\n",
       "      <td>Porphyromonas gingivalis</td>\n",
       "      <td>Porphyromonas gingivalis ATCC 33277</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>41</th>\n",
       "      <td>NC_010730</td>\n",
       "      <td>436114</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificales</td>\n",
       "      <td>Hydrogenothermaceae</td>\n",
       "      <td>Sulfurihydrogenibium</td>\n",
       "      <td>Sulfurihydrogenibium sp. YO3AOP1</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>42</th>\n",
       "      <td>CP001097</td>\n",
       "      <td>290315</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chlorobi</td>\n",
       "      <td>Chlorobia</td>\n",
       "      <td>Chlorobiales</td>\n",
       "      <td>Chlorobiaceae</td>\n",
       "      <td>Chlorobium</td>\n",
       "      <td>Chlorobium limicola</td>\n",
       "      <td>Chlorobium limicola DSM 245</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>43</th>\n",
       "      <td>CP001110</td>\n",
       "      <td>324925</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chlorobi</td>\n",
       "      <td>Chlorobia</td>\n",
       "      <td>Chlorobiales</td>\n",
       "      <td>Chlorobiaceae</td>\n",
       "      <td>Pelodictyon</td>\n",
       "      <td>Pelodictyon phaeoclathratiforme</td>\n",
       "      <td>Pelodictyon phaeoclathratiforme BU-1</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>44</th>\n",
       "      <td>CP001130</td>\n",
       "      <td>380749</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificales</td>\n",
       "      <td>Aquificaceae</td>\n",
       "      <td>Hydrogenobaculum</td>\n",
       "      <td>Hydrogenobaculum sp. Y04AAS1</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>45</th>\n",
       "      <td>NZ_CH959311</td>\n",
       "      <td>52598</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Alphaproteobacteria</td>\n",
       "      <td>Rhodobacterales</td>\n",
       "      <td>Rhodobacteraceae</td>\n",
       "      <td>Sulfitobacter</td>\n",
       "      <td>Sulfitobacter sp. EE-36</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>46</th>\n",
       "      <td>NZ_CH959317</td>\n",
       "      <td>314267</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Alphaproteobacteria</td>\n",
       "      <td>Rhodobacterales</td>\n",
       "      <td>Rhodobacteraceae</td>\n",
       "      <td>Sulfitobacter</td>\n",
       "      <td>Sulfitobacter sp. NAS-14.1</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>47</th>\n",
       "      <td>CP001251</td>\n",
       "      <td>515635</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Dictyoglomi</td>\n",
       "      <td>Dictyoglomia</td>\n",
       "      <td>Dictyoglomales</td>\n",
       "      <td>Dictyoglomaceae</td>\n",
       "      <td>Dictyoglomus</td>\n",
       "      <td>Dictyoglomus turgidum</td>\n",
       "      <td>Dictyoglomus turgidum DSM 6724</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>48</th>\n",
       "      <td>NC_011663</td>\n",
       "      <td>407976</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Gammaproteobacteria</td>\n",
       "      <td>Alteromonadales</td>\n",
       "      <td>Shewanellaceae</td>\n",
       "      <td>Shewanella</td>\n",
       "      <td>Shewanella baltica</td>\n",
       "      <td>Shewanella baltica OS223</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>49</th>\n",
       "      <td>CP000916</td>\n",
       "      <td>309803</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Thermotogae</td>\n",
       "      <td>Thermotogae</td>\n",
       "      <td>Thermotogales</td>\n",
       "      <td>Thermotogaceae</td>\n",
       "      <td>Thermotoga</td>\n",
       "      <td>Thermotoga neapolitana</td>\n",
       "      <td>Thermotoga neapolitana DSM 4359</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>50</th>\n",
       "      <td>NZ_DS996397</td>\n",
       "      <td>411464</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Deltaproteobacteria</td>\n",
       "      <td>Desulfovibrionales</td>\n",
       "      <td>Desulfovibrionaceae</td>\n",
       "      <td>Desulfovibrio</td>\n",
       "      <td>Desulfovibrio piger</td>\n",
       "      <td>Desulfovibrio piger ATCC 29098</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>51</th>\n",
       "      <td>CP001230</td>\n",
       "      <td>123214</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificales</td>\n",
       "      <td>Hydrogenothermaceae</td>\n",
       "      <td>Persephonella</td>\n",
       "      <td>Persephonella marina</td>\n",
       "      <td>Persephonella marina EX-H1</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>52</th>\n",
       "      <td>CP001472</td>\n",
       "      <td>240015</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Acidobacteria</td>\n",
       "      <td>Acidobacteriia</td>\n",
       "      <td>Acidobacteriales</td>\n",
       "      <td>Acidobacteriaceae</td>\n",
       "      <td>Acidobacterium</td>\n",
       "      <td>Acidobacterium capsulatum</td>\n",
       "      <td>Acidobacterium capsulatum ATCC 51196</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>53</th>\n",
       "      <td>AP009153</td>\n",
       "      <td>379066</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Gemmatimonadetes</td>\n",
       "      <td>Gemmatimonadetes</td>\n",
       "      <td>Gemmatimonadales</td>\n",
       "      <td>Gemmatimonadaceae</td>\n",
       "      <td>Gemmatimonas</td>\n",
       "      <td>Gemmatimonas aurantiaca</td>\n",
       "      <td>Gemmatimonas aurantiaca T-27</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>54</th>\n",
       "      <td>CP001941</td>\n",
       "      <td>439481</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>NaN</td>\n",
       "      <td>NaN</td>\n",
       "      <td>NaN</td>\n",
       "      <td>Aciduliprofundum</td>\n",
       "      <td>Aciduliprofundum boonei</td>\n",
       "      <td>Aciduliprofundum boonei T469</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>55</th>\n",
       "      <td>NC_013968</td>\n",
       "      <td>309800</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Euryarchaeota</td>\n",
       "      <td>Halobacteria</td>\n",
       "      <td>Haloferacales</td>\n",
       "      <td>Haloferacaceae</td>\n",
       "      <td>Haloferax</td>\n",
       "      <td>Haloferax volcanii</td>\n",
       "      <td>Haloferax volcanii DS2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>56</th>\n",
       "      <td>NZ_KE136524</td>\n",
       "      <td>226185</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Firmicutes</td>\n",
       "      <td>Bacilli</td>\n",
       "      <td>Lactobacillales</td>\n",
       "      <td>Enterococcaceae</td>\n",
       "      <td>Enterococcus</td>\n",
       "      <td>Enterococcus faecalis</td>\n",
       "      <td>Enterococcus faecalis V583</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>57</th>\n",
       "      <td>NZ_KQ961402</td>\n",
       "      <td>542</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Alphaproteobacteria</td>\n",
       "      <td>Sphingomonadales</td>\n",
       "      <td>Sphingomonadaceae</td>\n",
       "      <td>Zymomonas</td>\n",
       "      <td>Zymomonas mobilis</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>58</th>\n",
       "      <td>NZ_CP015081</td>\n",
       "      <td>243230</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Deinococcus-Thermus</td>\n",
       "      <td>Deinococci</td>\n",
       "      <td>Deinococcales</td>\n",
       "      <td>Deinococcaceae</td>\n",
       "      <td>Deinococcus</td>\n",
       "      <td>Deinococcus radiodurans</td>\n",
       "      <td>Deinococcus radiodurans R1</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>59</th>\n",
       "      <td>NZ_ABZS01000228</td>\n",
       "      <td>432331</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificae</td>\n",
       "      <td>Aquificales</td>\n",
       "      <td>Hydrogenothermaceae</td>\n",
       "      <td>Sulfurihydrogenibium</td>\n",
       "      <td>Sulfurihydrogenibium yellowstonense</td>\n",
       "      <td>Sulfurihydrogenibium yellowstonense SS-5</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>60</th>\n",
       "      <td>NZ_JGWU01000001</td>\n",
       "      <td>1458259</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Proteobacteria</td>\n",
       "      <td>Betaproteobacteria</td>\n",
       "      <td>Burkholderiales</td>\n",
       "      <td>Alcaligenaceae</td>\n",
       "      <td>Bordetella</td>\n",
       "      <td>Bordetella bronchiseptica</td>\n",
       "      <td>Bordetella bronchiseptica D989</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>61</th>\n",
       "      <td>NZ_FWDH01000003</td>\n",
       "      <td>31899</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Firmicutes</td>\n",
       "      <td>Clostridia</td>\n",
       "      <td>Thermoanaerobacterales</td>\n",
       "      <td>Thermoanaerobacterales Family III. Incertae Sedis</td>\n",
       "      <td>Caldicellulosiruptor</td>\n",
       "      <td>Caldicellulosiruptor bescii</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>62</th>\n",
       "      <td>NC_009972</td>\n",
       "      <td>316274</td>\n",
       "      <td>Bacteria</td>\n",
       "      <td>Chloroflexi</td>\n",
       "      <td>Chloroflexia</td>\n",
       "      <td>Herpetosiphonales</td>\n",
       "      <td>Herpetosiphonaceae</td>\n",
       "      <td>Herpetosiphon</td>\n",
       "      <td>Herpetosiphon aurantiacus</td>\n",
       "      <td>Herpetosiphon aurantiacus DSM 785</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>63</th>\n",
       "      <td>NC_005213</td>\n",
       "      <td>228908</td>\n",
       "      <td>Archaea</td>\n",
       "      <td>Nanoarchaeota</td>\n",
       "      <td>NaN</td>\n",
       "      <td>Nanoarchaeales</td>\n",
       "      <td>Nanoarchaeaceae</td>\n",
       "      <td>Nanoarchaeum</td>\n",
       "      <td>Nanoarchaeum equitans</td>\n",
       "      <td>Nanoarchaeum equitans Kin4-M</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>64 rows × 10 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "          accession    taxid superkingdom               phylum  \\\n",
       "0          AE000782   224325      Archaea        Euryarchaeota   \n",
       "1         NC_000909   243232      Archaea        Euryarchaeota   \n",
       "2         NC_003272   103690     Bacteria        Cyanobacteria   \n",
       "3          AE009441   178306      Archaea        Crenarchaeota   \n",
       "4          AE009950   186497      Archaea        Euryarchaeota   \n",
       "5          AE009951   190304     Bacteria         Fusobacteria   \n",
       "6          AE010299   188937      Archaea        Euryarchaeota   \n",
       "7          AE009439   190192      Archaea        Euryarchaeota   \n",
       "8         NC_003911   246200     Bacteria       Proteobacteria   \n",
       "9          AE006470   194439     Bacteria             Chlorobi   \n",
       "10         AE015928   226186     Bacteria        Bacteroidetes   \n",
       "11         AL954747   228410     Bacteria       Proteobacteria   \n",
       "12         BX119912   243090     Bacteria       Planctomycetes   \n",
       "13         BX571656   273121     Bacteria       Proteobacteria   \n",
       "14         AE017180   243231     Bacteria       Proteobacteria   \n",
       "15         AE017226   243275     Bacteria         Spirochaetes   \n",
       "16         BX950229   267377      Archaea        Euryarchaeota   \n",
       "17         AE017221   262724     Bacteria  Deinococcus-Thermus   \n",
       "18         BA000001    70601      Archaea        Euryarchaeota   \n",
       "19         BA000023   273063      Archaea        Crenarchaeota   \n",
       "20        NC_007951   266265     Bacteria       Proteobacteria   \n",
       "21         CP000492   290317     Bacteria             Chlorobi   \n",
       "22        NC_008751   391774     Bacteria       Proteobacteria   \n",
       "23         CP000568   203119     Bacteria           Firmicutes   \n",
       "24         CP000561   410359      Archaea        Crenarchaeota   \n",
       "25         CP000609   402880      Archaea        Euryarchaeota   \n",
       "26         CP000607   290318     Bacteria             Chlorobi   \n",
       "27         CP000660   340102      Archaea        Crenarchaeota   \n",
       "28         CP000667   369723     Bacteria       Actinobacteria   \n",
       "29         CP000679   351627     Bacteria           Firmicutes   \n",
       "..              ...      ...          ...                  ...   \n",
       "34         CP000850   391037     Bacteria       Actinobacteria   \n",
       "35         CP000909   324602     Bacteria          Chloroflexi   \n",
       "36         CP000924   340099     Bacteria           Firmicutes   \n",
       "37         CP000969   126740     Bacteria          Thermotogae   \n",
       "38         CP001013   395495     Bacteria       Proteobacteria   \n",
       "39         CP001071   349741     Bacteria      Verrucomicrobia   \n",
       "40         AP009380   431947     Bacteria        Bacteroidetes   \n",
       "41        NC_010730   436114     Bacteria            Aquificae   \n",
       "42         CP001097   290315     Bacteria             Chlorobi   \n",
       "43         CP001110   324925     Bacteria             Chlorobi   \n",
       "44         CP001130   380749     Bacteria            Aquificae   \n",
       "45      NZ_CH959311    52598     Bacteria       Proteobacteria   \n",
       "46      NZ_CH959317   314267     Bacteria       Proteobacteria   \n",
       "47         CP001251   515635     Bacteria          Dictyoglomi   \n",
       "48        NC_011663   407976     Bacteria       Proteobacteria   \n",
       "49         CP000916   309803     Bacteria          Thermotogae   \n",
       "50      NZ_DS996397   411464     Bacteria       Proteobacteria   \n",
       "51         CP001230   123214     Bacteria            Aquificae   \n",
       "52         CP001472   240015     Bacteria        Acidobacteria   \n",
       "53         AP009153   379066     Bacteria     Gemmatimonadetes   \n",
       "54         CP001941   439481      Archaea        Euryarchaeota   \n",
       "55        NC_013968   309800      Archaea        Euryarchaeota   \n",
       "56      NZ_KE136524   226185     Bacteria           Firmicutes   \n",
       "57      NZ_KQ961402      542     Bacteria       Proteobacteria   \n",
       "58      NZ_CP015081   243230     Bacteria  Deinococcus-Thermus   \n",
       "59  NZ_ABZS01000228   432331     Bacteria            Aquificae   \n",
       "60  NZ_JGWU01000001  1458259     Bacteria       Proteobacteria   \n",
       "61  NZ_FWDH01000003    31899     Bacteria           Firmicutes   \n",
       "62        NC_009972   316274     Bacteria          Chloroflexi   \n",
       "63        NC_005213   228908      Archaea        Nanoarchaeota   \n",
       "\n",
       "                    class                   order  \\\n",
       "0            Archaeoglobi         Archaeoglobales   \n",
       "1            Methanococci         Methanococcales   \n",
       "2                     NaN              Nostocales   \n",
       "3            Thermoprotei         Thermoproteales   \n",
       "4             Thermococci          Thermococcales   \n",
       "5           Fusobacteriia         Fusobacteriales   \n",
       "6         Methanomicrobia       Methanosarcinales   \n",
       "7             Methanopyri          Methanopyrales   \n",
       "8     Alphaproteobacteria         Rhodobacterales   \n",
       "9               Chlorobia            Chlorobiales   \n",
       "10            Bacteroidia           Bacteroidales   \n",
       "11     Betaproteobacteria        Nitrosomonadales   \n",
       "12         Planctomycetia        Planctomycetales   \n",
       "13  Epsilonproteobacteria       Campylobacterales   \n",
       "14    Deltaproteobacteria      Desulfuromonadales   \n",
       "15           Spirochaetia          Spirochaetales   \n",
       "16           Methanococci         Methanococcales   \n",
       "17             Deinococci               Thermales   \n",
       "18            Thermococci          Thermococcales   \n",
       "19           Thermoprotei            Sulfolobales   \n",
       "20     Betaproteobacteria         Burkholderiales   \n",
       "21              Chlorobia            Chlorobiales   \n",
       "22    Deltaproteobacteria      Desulfovibrionales   \n",
       "23             Clostridia           Clostridiales   \n",
       "24           Thermoprotei         Thermoproteales   \n",
       "25           Methanococci         Methanococcales   \n",
       "26              Chlorobia            Chlorobiales   \n",
       "27           Thermoprotei         Thermoproteales   \n",
       "28         Actinobacteria       Micromonosporales   \n",
       "29             Clostridia  Thermoanaerobacterales   \n",
       "..                    ...                     ...   \n",
       "34         Actinobacteria       Micromonosporales   \n",
       "35           Chloroflexia          Chloroflexales   \n",
       "36             Clostridia  Thermoanaerobacterales   \n",
       "37            Thermotogae           Thermotogales   \n",
       "38     Betaproteobacteria         Burkholderiales   \n",
       "39       Verrucomicrobiae      Verrucomicrobiales   \n",
       "40            Bacteroidia           Bacteroidales   \n",
       "41              Aquificae             Aquificales   \n",
       "42              Chlorobia            Chlorobiales   \n",
       "43              Chlorobia            Chlorobiales   \n",
       "44              Aquificae             Aquificales   \n",
       "45    Alphaproteobacteria         Rhodobacterales   \n",
       "46    Alphaproteobacteria         Rhodobacterales   \n",
       "47           Dictyoglomia          Dictyoglomales   \n",
       "48    Gammaproteobacteria         Alteromonadales   \n",
       "49            Thermotogae           Thermotogales   \n",
       "50    Deltaproteobacteria      Desulfovibrionales   \n",
       "51              Aquificae             Aquificales   \n",
       "52         Acidobacteriia        Acidobacteriales   \n",
       "53       Gemmatimonadetes        Gemmatimonadales   \n",
       "54                    NaN                     NaN   \n",
       "55           Halobacteria           Haloferacales   \n",
       "56                Bacilli         Lactobacillales   \n",
       "57    Alphaproteobacteria        Sphingomonadales   \n",
       "58             Deinococci           Deinococcales   \n",
       "59              Aquificae             Aquificales   \n",
       "60     Betaproteobacteria         Burkholderiales   \n",
       "61             Clostridia  Thermoanaerobacterales   \n",
       "62           Chloroflexia       Herpetosiphonales   \n",
       "63                    NaN          Nanoarchaeales   \n",
       "\n",
       "                                               family                 genus  \\\n",
       "0                                    Archaeoglobaceae         Archaeoglobus   \n",
       "1                               Methanocaldococcaceae    Methanocaldococcus   \n",
       "2                                         Nostocaceae                Nostoc   \n",
       "3                                    Thermoproteaceae           Pyrobaculum   \n",
       "4                                     Thermococcaceae            Pyrococcus   \n",
       "5                                    Fusobacteriaceae         Fusobacterium   \n",
       "6                                  Methanosarcinaceae        Methanosarcina   \n",
       "7                                     Methanopyraceae          Methanopyrus   \n",
       "8                                    Rhodobacteraceae              Ruegeria   \n",
       "9                                       Chlorobiaceae         Chlorobaculum   \n",
       "10                                     Bacteroidaceae           Bacteroides   \n",
       "11                                  Nitrosomonadaceae          Nitrosomonas   \n",
       "12                                  Planctomycetaceae        Rhodopirellula   \n",
       "13                                  Helicobacteraceae             Wolinella   \n",
       "14                                     Geobacteraceae             Geobacter   \n",
       "15                                    Spirochaetaceae             Treponema   \n",
       "16                                   Methanococcaceae         Methanococcus   \n",
       "17                                         Thermaceae               Thermus   \n",
       "18                                    Thermococcaceae            Pyrococcus   \n",
       "19                                      Sulfolobaceae            Sulfolobus   \n",
       "20                                   Burkholderiaceae      Paraburkholderia   \n",
       "21                                      Chlorobiaceae            Chlorobium   \n",
       "22                                Desulfovibrionaceae         Desulfovibrio   \n",
       "23                                    Ruminococcaceae     Ruminiclostridium   \n",
       "24                                   Thermoproteaceae           Pyrobaculum   \n",
       "25                                   Methanococcaceae         Methanococcus   \n",
       "26                                      Chlorobiaceae            Chlorobium   \n",
       "27                                   Thermoproteaceae           Pyrobaculum   \n",
       "28                                 Micromonosporaceae           Salinispora   \n",
       "29  Thermoanaerobacterales Family III. Incertae Sedis  Caldicellulosiruptor   \n",
       "..                                                ...                   ...   \n",
       "34                                 Micromonosporaceae           Salinispora   \n",
       "35                                    Chloroflexaceae          Chloroflexus   \n",
       "36                            Thermoanaerobacteraceae    Thermoanaerobacter   \n",
       "37                                     Thermotogaceae            Thermotoga   \n",
       "38                                                NaN            Leptothrix   \n",
       "39                                    Akkermansiaceae           Akkermansia   \n",
       "40                                 Porphyromonadaceae         Porphyromonas   \n",
       "41                                Hydrogenothermaceae  Sulfurihydrogenibium   \n",
       "42                                      Chlorobiaceae            Chlorobium   \n",
       "43                                      Chlorobiaceae           Pelodictyon   \n",
       "44                                       Aquificaceae      Hydrogenobaculum   \n",
       "45                                   Rhodobacteraceae         Sulfitobacter   \n",
       "46                                   Rhodobacteraceae         Sulfitobacter   \n",
       "47                                    Dictyoglomaceae          Dictyoglomus   \n",
       "48                                     Shewanellaceae            Shewanella   \n",
       "49                                     Thermotogaceae            Thermotoga   \n",
       "50                                Desulfovibrionaceae         Desulfovibrio   \n",
       "51                                Hydrogenothermaceae         Persephonella   \n",
       "52                                  Acidobacteriaceae        Acidobacterium   \n",
       "53                                  Gemmatimonadaceae          Gemmatimonas   \n",
       "54                                                NaN      Aciduliprofundum   \n",
       "55                                     Haloferacaceae             Haloferax   \n",
       "56                                    Enterococcaceae          Enterococcus   \n",
       "57                                  Sphingomonadaceae             Zymomonas   \n",
       "58                                     Deinococcaceae           Deinococcus   \n",
       "59                                Hydrogenothermaceae  Sulfurihydrogenibium   \n",
       "60                                     Alcaligenaceae            Bordetella   \n",
       "61  Thermoanaerobacterales Family III. Incertae Sedis  Caldicellulosiruptor   \n",
       "62                                 Herpetosiphonaceae         Herpetosiphon   \n",
       "63                                    Nanoarchaeaceae          Nanoarchaeum   \n",
       "\n",
       "                                 species  \\\n",
       "0                 Archaeoglobus fulgidus   \n",
       "1          Methanocaldococcus jannaschii   \n",
       "2                    Nostoc sp. PCC 7120   \n",
       "3                 Pyrobaculum aerophilum   \n",
       "4                    Pyrococcus furiosus   \n",
       "5                Fusobacterium nucleatum   \n",
       "6             Methanosarcina acetivorans   \n",
       "7                  Methanopyrus kandleri   \n",
       "8                      Ruegeria pomeroyi   \n",
       "9                  Chlorobaculum tepidum   \n",
       "10          Bacteroides thetaiotaomicron   \n",
       "11                 Nitrosomonas europaea   \n",
       "12                Rhodopirellula baltica   \n",
       "13                Wolinella succinogenes   \n",
       "14              Geobacter sulfurreducens   \n",
       "15                   Treponema denticola   \n",
       "16             Methanococcus maripaludis   \n",
       "17                  Thermus thermophilus   \n",
       "18                 Pyrococcus horikoshii   \n",
       "19                   Sulfolobus tokodaii   \n",
       "20           Paraburkholderia xenovorans   \n",
       "21           Chlorobium phaeobacteroides   \n",
       "22                Desulfovibrio vulgaris   \n",
       "23        Ruminiclostridium thermocellum   \n",
       "24              Pyrobaculum calidifontis   \n",
       "25             Methanococcus maripaludis   \n",
       "26            Chlorobium phaeovibrioides   \n",
       "27               Pyrobaculum arsenaticum   \n",
       "28                   Salinispora tropica   \n",
       "29  Caldicellulosiruptor saccharolyticus   \n",
       "..                                   ...   \n",
       "34                 Salinispora arenicola   \n",
       "35              Chloroflexus aurantiacus   \n",
       "36   Thermoanaerobacter pseudethanolicus   \n",
       "37                    Thermotoga sp. RQ2   \n",
       "38                  Leptothrix cholodnii   \n",
       "39               Akkermansia muciniphila   \n",
       "40              Porphyromonas gingivalis   \n",
       "41      Sulfurihydrogenibium sp. YO3AOP1   \n",
       "42                   Chlorobium limicola   \n",
       "43       Pelodictyon phaeoclathratiforme   \n",
       "44          Hydrogenobaculum sp. Y04AAS1   \n",
       "45               Sulfitobacter sp. EE-36   \n",
       "46            Sulfitobacter sp. NAS-14.1   \n",
       "47                 Dictyoglomus turgidum   \n",
       "48                    Shewanella baltica   \n",
       "49                Thermotoga neapolitana   \n",
       "50                   Desulfovibrio piger   \n",
       "51                  Persephonella marina   \n",
       "52             Acidobacterium capsulatum   \n",
       "53               Gemmatimonas aurantiaca   \n",
       "54               Aciduliprofundum boonei   \n",
       "55                    Haloferax volcanii   \n",
       "56                 Enterococcus faecalis   \n",
       "57                     Zymomonas mobilis   \n",
       "58               Deinococcus radiodurans   \n",
       "59   Sulfurihydrogenibium yellowstonense   \n",
       "60             Bordetella bronchiseptica   \n",
       "61           Caldicellulosiruptor bescii   \n",
       "62             Herpetosiphon aurantiacus   \n",
       "63                 Nanoarchaeum equitans   \n",
       "\n",
       "                                            strain  \n",
       "0                  Archaeoglobus fulgidus DSM 4304  \n",
       "1           Methanocaldococcus jannaschii DSM 2661  \n",
       "2                                              NaN  \n",
       "3                  Pyrobaculum aerophilum str. IM2  \n",
       "4                     Pyrococcus furiosus DSM 3638  \n",
       "5                                              NaN  \n",
       "6                   Methanosarcina acetivorans C2A  \n",
       "7                       Methanopyrus kandleri AV19  \n",
       "8                          Ruegeria pomeroyi DSS-3  \n",
       "9                        Chlorobaculum tepidum TLS  \n",
       "10           Bacteroides thetaiotaomicron VPI-5482  \n",
       "11                Nitrosomonas europaea ATCC 19718  \n",
       "12                     Rhodopirellula baltica SH 1  \n",
       "13                 Wolinella succinogenes DSM 1740  \n",
       "14                    Geobacter sulfurreducens PCA  \n",
       "15                  Treponema denticola ATCC 35405  \n",
       "16                    Methanococcus maripaludis S2  \n",
       "17                       Thermus thermophilus HB27  \n",
       "18                       Pyrococcus horikoshii OT3  \n",
       "19                      Sulfolobus tokodaii str. 7  \n",
       "20               Paraburkholderia xenovorans LB400  \n",
       "21             Chlorobium phaeobacteroides DSM 266  \n",
       "22                      Desulfovibrio vulgaris DP4  \n",
       "23       Ruminiclostridium thermocellum ATCC 27405  \n",
       "24              Pyrobaculum calidifontis JCM 11548  \n",
       "25                    Methanococcus maripaludis C5  \n",
       "26              Chlorobium phaeovibrioides DSM 265  \n",
       "27               Pyrobaculum arsenaticum DSM 13514  \n",
       "28                     Salinispora tropica CNB-440  \n",
       "29   Caldicellulosiruptor saccharolyticus DSM 8903  \n",
       "..                                             ...  \n",
       "34                   Salinispora arenicola CNS-205  \n",
       "35                Chloroflexus aurantiacus J-10-fl  \n",
       "36  Thermoanaerobacter pseudethanolicus ATCC 33223  \n",
       "37                                             NaN  \n",
       "38                       Leptothrix cholodnii SP-6  \n",
       "39            Akkermansia muciniphila ATCC BAA-835  \n",
       "40             Porphyromonas gingivalis ATCC 33277  \n",
       "41                                             NaN  \n",
       "42                     Chlorobium limicola DSM 245  \n",
       "43            Pelodictyon phaeoclathratiforme BU-1  \n",
       "44                                             NaN  \n",
       "45                                             NaN  \n",
       "46                                             NaN  \n",
       "47                  Dictyoglomus turgidum DSM 6724  \n",
       "48                        Shewanella baltica OS223  \n",
       "49                 Thermotoga neapolitana DSM 4359  \n",
       "50                  Desulfovibrio piger ATCC 29098  \n",
       "51                      Persephonella marina EX-H1  \n",
       "52            Acidobacterium capsulatum ATCC 51196  \n",
       "53                    Gemmatimonas aurantiaca T-27  \n",
       "54                    Aciduliprofundum boonei T469  \n",
       "55                          Haloferax volcanii DS2  \n",
       "56                      Enterococcus faecalis V583  \n",
       "57                                             NaN  \n",
       "58                      Deinococcus radiodurans R1  \n",
       "59        Sulfurihydrogenibium yellowstonense SS-5  \n",
       "60                  Bordetella bronchiseptica D989  \n",
       "61                                             NaN  \n",
       "62               Herpetosiphon aurantiacus DSM 785  \n",
       "63                    Nanoarchaeum equitans Kin4-M  \n",
       "\n",
       "[64 rows x 10 columns]"
      ]
     },
     "execution_count": 9,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "import pandas\n",
    "df = pandas.read_csv('podar-lineage.csv')\n",
    "df"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kexamining spreadsheet headers...\n",
      "\u001b[K** assuming column 'accession' is identifiers in spreadsheet\n",
      "\u001b[K64 distinct identities in spreadsheet out of 64 rows.\n",
      "\u001b[K64 distinct lineages in spreadsheet out of 64 rows.\n",
      "\u001b[K64 assigned lineages out of 64 distinct lineages in spreadsheet. 64)\n",
      "\u001b[K64 identifiers used out of 64 distinct identifiers in spreadsheet.\n",
      "\u001b[Ksaving to LCA DB: taxdb.lca.json\n"
     ]
    }
   ],
   "source": [
    "!sourmash lca index podar-lineage.csv taxdb big_genomes/*.sig  -C 3 --split-identifiers"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "This database 'taxdb.lca.json' can be used for search and gather as above:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kselect query k=31 automatically.\n",
      "\u001b[Kloaded query: fake-metagenome.fa... (k=31, DNA)\n",
      "\u001b[Kloaded 1 databases.                                                            \n",
      "\n",
      "\n",
      "overlap     p_query p_match\n",
      "---------   ------- -------\n",
      "0.6 Mbp       46.7%   11.6%    NC_011663.1 Shewanella baltica OS223,...\n",
      "0.5 Mbp       38.7%   19.3%    CP001071.1 Akkermansia muciniphila AT...\n",
      "0.5 Mbp       14.6%    3.9%    NC_009665.1 Shewanella baltica OS185,...\n",
      "\n",
      "found 3 matches total;\n",
      "the recovered matches hit 100.0% of the query\n",
      "\n"
     ]
    }
   ],
   "source": [
    "!sourmash gather fake-metagenome.fa.sig taxdb.lca.json"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "...but can also be used for taxonomic summarization:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\u001b[K== This is sourmash version 2.0.0a12.dev48+ga92289b. ==\n",
      "\u001b[K== Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==\n",
      "\n",
      "\u001b[Kloaded 1 LCA databases. ksize=31, scaled=10000\n",
      "\u001b[Kfinding query signatures...\n",
      "\u001b[Kloaded 1 signatures from 1 files total.of 1)\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae;Verrucomicrobiales;Akkermansiaceae;Akkermansia;Akkermansia muciniphila;Akkermansia muciniphila ATCC BAA-835\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae;Verrucomicrobiales;Akkermansiaceae;Akkermansia;Akkermansia muciniphila\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae;Verrucomicrobiales;Akkermansiaceae;Akkermansia\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae;Verrucomicrobiales;Akkermansiaceae\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae;Verrucomicrobiales\n",
      "38.7%    53   Bacteria;Verrucomicrobia;Verrucomicrobiae\n",
      "38.7%    53   Bacteria;Verrucomicrobia\n",
      "100.0%   137   Bacteria\n",
      "61.3%    84   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Shewanellaceae;Shewanella;Shewanella baltica\n",
      "61.3%    84   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Shewanellaceae;Shewanella\n",
      "61.3%    84   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Shewanellaceae\n",
      "61.3%    84   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales\n",
      "61.3%    84   Bacteria;Proteobacteria;Gammaproteobacteria\n",
      "61.3%    84   Bacteria;Proteobacteria\n",
      "22.6%    31   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Shewanellaceae;Shewanella;Shewanella baltica;Shewanella baltica OS223\n",
      "14.6%    20   Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Shewanellaceae;Shewanella;Shewanella baltica;Shewanella baltica OS185\n"
     ]
    }
   ],
   "source": [
    "!sourmash lca summarize --query fake-metagenome.fa.sig --db taxdb.lca.json"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## A full list of notebooks\n",
    "\n",
    "[An introduction to k-mers for genome comparison and analysis](kmers-and-minhash.ipynb)\n",
    "\n",
    "[Some sourmash command line examples!](sourmash-examples.ipynb)\n",
    "\n",
    "[Working with private collections of signatures.](sourmash-collections.ipynb)"
   ]
  }
 ],
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