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compute-input-prot-another-way.py
executable file
·146 lines (120 loc) · 2.65 KB
/
compute-input-prot-another-way.py
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#! /usr/bin/env python
"""
Use the MurmurHash library mmh3 and separate Python code to calculate
a MinHash signature for input protein sequence, as a way to do an
external check on our C++ implementation.
The output of this is used in test_sourmash.py to verify our C++ code.
"""
dna_to_aa = {
"TTT": "F",
"TTC": "F",
"TTA": "L",
"TTG": "L",
"TCT": "S",
"TCC": "S",
"TCA": "S",
"TCG": "S",
"TAT": "Y",
"TAC": "Y",
"TAA": "*",
"TAG": "*",
"TGA": "*",
"TGT": "C",
"TGC": "C",
"TGG": "W",
"CTT": "L",
"CTC": "L",
"CTA": "L",
"CTG": "L",
"CCT": "P",
"CCC": "P",
"CCA": "P",
"CCG": "P",
"CAT": "H",
"CAC": "H",
"CAA": "Q",
"CAG": "Q",
"CGT": "R",
"CGC": "R",
"CGA": "R",
"CGG": "R",
"ATT": "I",
"ATC": "I",
"ATA": "I",
"ATG": "M",
"ACT": "T",
"ACC": "T",
"ACA": "T",
"ACG": "T",
"AAT": "N",
"AAC": "N",
"AAA": "K",
"AAG": "K",
"AGT": "S",
"AGC": "S",
"AGA": "R",
"AGG": "R",
"GTT": "V",
"GTC": "V",
"GTA": "V",
"GTG": "V",
"GCT": "A",
"GCC": "A",
"GCA": "A",
"GCG": "A",
"GAT": "D",
"GAC": "D",
"GAA": "E",
"GAG": "E",
"GGT": "G",
"GGC": "G",
"GGA": "G",
"GGG": "G",
}
__complementTranslation = {"A": "T", "C": "G", "G": "C", "T": "A", "N": "N"}
def complement(s):
"""
Return complement of 's'.
"""
c = "".join(__complementTranslation[n] for n in s)
return c
def reverse(s):
"""
Return reverse of 's'.
"""
r = "".join(reversed(s))
return r
def peptides(seq, start):
for i in range(start, len(seq), 3):
yield dna_to_aa.get(seq[i : i + 3], "X")
def translate(seq):
for i in range(3):
pep = peptides(seq, i)
yield "".join(pep)
revcomp = reverse(complement(seq))
for i in range(3):
pep = peptides(revcomp, i)
yield "".join(pep)
def kmers(seq, k):
for start in range(len(seq) - k + 1):
yield seq[start : start + k]
###
K = 21
import sys
import screed
import mmh3
import sourmash
print("imported sourmash:", sourmash, file=sys.stderr)
import sourmash.signature
record = next(iter(screed.open(sys.argv[1])))
print("loaded", record.name, file=sys.stderr)
mh = sourmash.MinHash(ksize=K, n=500, is_protein=True)
prot_ksize = int(K / 3)
for kmer in kmers(record.sequence, prot_ksize):
hash = mmh3.hash64(kmer, seed=42)[0]
# convert to unsigned int if negative
if hash < 0:
hash += 2**64
mh.add_hash(hash)
s = sourmash.signature.SourmashSignature("", mh, name=record.name)
print(sourmash.signature.save_signatures([s]))