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clean up
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srobb1 committed Mar 16, 2013
1 parent 424b8ec commit 6c1cb50
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1 change: 1 addition & 0 deletions README.md
Expand Up @@ -452,3 +452,4 @@ For any of the listed reasons, or anything else, please leave us a <a href="http

<a href="https://github.com/srobb1/RelocaTE/issues?page=1&sort=comments&state=open">Leave a message here</a>


35 changes: 18 additions & 17 deletions sample_relocaTE_run/sample_readme.txt
Expand Up @@ -9,6 +9,7 @@ These scripts expect that the following programs are installed and included in y
3. BWA
4. BioPerl
5. Blat
6. Blast: formatdb and fastacmd

STEPS A-C
A. RelocaTE
Expand All @@ -32,32 +33,32 @@ A. To find TE insertions in the included fastq reads run RelocaTE:
or
sh run_relocaTE.sh

These scritps will run relocaTE directly or through a series of shell scripts.
If you are running
These scripts will run relocaTE directly or through a series of shell scripts.
If you are running
- run_relocaTE_qsub.sh:
1) follow the instructions that are printed to the screen (run run_these_jobs.sh)
2) once complete, view the results in 02052012_sample/mping/results
1) follow the instructions that are printed to the screen (run run_these_jobs.sh)
2) once complete, view the results in 02052012_sample/mping/results
or
- run_relocaTE_shell.sh
1) follow the instructions that are printed to the screen (run run_these_jobs.sh)
2) once complete, view the results in 02052012_sample/mping/results
1) follow the instructions that are printed to the screen (run run_these_jobs.sh)
2) once complete, view the results in 02052012_sample/mping/results
or
- run_relocaTE.sh
1) once complete, view the results in 02052012_sample/mping/results
1) once complete, view the results in 02052012_sample/mping/results


B. Find Spanners to help classify the insertions (homozygous, heterozygous, etc) by generating a BAM file of the reads not trimmed of TE to the referenc
e.
B. Find Spanners to help classify the insertions (homozygous, heterozygous, etc) by generating a BAM file of the reads not trimmed of TE to the reference.
A BAM file is included in the sample data set, but one can be genreted by running the included script:
create_bam.sh by:
- changing directory into the directory of this script
- and typing "sh create_bam.sh"
create_bam.sh by:
- changing directory into the directory of this script
- and typing "sh create_bam.sh"


C. To classify the insertions (homozygous, heterozygous, etc) run_characTErizer.sh
- change directory into the directory of this script
- type "sh run_characTErizer.sh"
- change directory into the directory of this script
- type "sh run_characTErizer.sh"
- once complete, view the resulting files in the directory you ran characTErizer.pl
1) sample.inserts_characTErized.gff: GFF file of the classified insertions including excisions
2) sample.inserts_characTErized.txt: Text file of the classified insertions including excisions
3) excisions_with_footprint.vcfinfo: additional information on the insertions that have been classified as exicision events
1) sample.inserts_characTErized.gff: GFF file of the classified insertions including excisions
2) sample.inserts_characTErized.txt: Text file of the classified insertions including excisions
3) excisions_with_footprint.vcfinfo: additional information on the insertions that have been classified as exicision events

1 change: 1 addition & 0 deletions scripts/characterizer.pl
Expand Up @@ -310,3 +310,4 @@ sub getHelp {
}
}
}

312 changes: 0 additions & 312 deletions scripts/characterizer.pl~

This file was deleted.

4 changes: 2 additions & 2 deletions scripts/relocaTE.pl
Expand Up @@ -147,8 +147,8 @@
}
else {
my $fq_path = File::Spec->rel2abs($fq_dir);
@fq_files = <$fq_path/*fq>;
my @fastq_files = <$fq_path/*fastq>;
@fq_files = <$fq_path/*.fq>;
my @fastq_files = <$fq_path/*.fastq>;
push @fq_files, @fastq_files;
if ( scalar @fq_files == 0 ) {
print "Must provide at least 1 short read file\n";
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