mag_incubate.json

{
  "refs": {
    "pcr_6": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_5": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_4": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_3": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_2": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_1": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "pcr_0": {
      "new": "96-pcr", 
      "store": {
        "where": "cold_20"
      }
    }, 
    "test": {
      "new": "96-flat", 
      "discard": true
    }
  }, 
  "instructions": [
    {
      "groups": [
        [
          {
            "release": {
              "duration": "30.0:second", 
              "object": "pcr_0", 
              "frequency": "1.0:hertz", 
              "center": 0.05, 
              "amplitude": 0
            }
          }
        ]
      ], 
      "magnetic_head": "96-pcr", 
      "op": "magnetic_transfer"
    }, 
    {
      "groups": [
        {
          "distribute": {
            "to": [
              {
                "volume": "30.0:microliter", 
                "well": "test/7"
              }, 
              {
                "volume": "30.0:microliter", 
                "well": "test/8"
              }, 
              {
                "volume": "30.0:microliter", 
                "well": "test/9"
              }
            ], 
            "from": "test/1", 
            "allow_carryover": true
          }
        }, 
        {
          "distribute": {
            "to": [
              {
                "volume": "30.0:microliter", 
                "well": "test/10"
              }
            ], 
            "from": "test/2", 
            "allow_carryover": true
          }
        }, 
        {
          "distribute": {
            "to": [
              {
                "volume": "5.0:microliter", 
                "well": "test/1"
              }
            ], 
            "from": "test/0", 
            "allow_carryover": false
          }
        }
      ], 
      "op": "pipette"
    }, 
    {
      "groups": [
        [
          {
            "incubate": {
              "duration": "30.0:minute", 
              "tip_position": 1.5, 
              "object": "pcr_0", 
              "magnetize": false, 
              "temperature": "30.0:celsius"
            }
          }
        ]
      ], 
      "magnetic_head": "96-pcr", 
      "op": "magnetic_transfer"
    }
  ]
}