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How to use FlowCal's Excel UI

To use the FlowCal’s Excel UI, follow these steps:

  1. Make and save an Excel file indicating the FCS files to process. Click here <input_format> for information on how to make a properly formatted input Excel file.
  2. Launch FlowCal’s Excel UI by double clicking on Run FlowCal (Windows).bat or Run FlowCal (macOS).
  3. A window will appear requesting an input Excel file. Locate the Excel file made in step 1 and click on “Open”.
  4. FlowCal will start processing<analysis> the indicated calibration beads and cell samples. A terminal window will appear indicating the progress of the analysis.
  5. When the analysis finishes, the message “Press Enter to finish...” will appear. Press Enter and close the terminal window. A set of plots<excel-ui-outputs-plots> and an Excel file<excel-ui-outputs-excel> with statistics will appear in the same directory in which the input Excel file was located.