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error when I run ge_stat() code of metan package #21
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@nivelive please, provide a reproducible example so that I can reproduce the error on my side. |
setwd("D:/PhD/Ragi")
library(readxl)
library(metan)
#> Registered S3 method overwritten by 'GGally':
#> method from
#> +.gg ggplot2
#> |=========================================================|
#> | Multi-Environment Trial Analysis (metan) v1.17.0 |
#> | Author: Tiago Olivoto |
#> | Type 'citation('metan')' to know how to cite metan |
#> | Type 'vignette('metan_start')' for a short tutorial |
#> | Visit 'https://bit.ly/pkgmetan' for a complete tutorial |
#> |=========================================================|
library(reprex)
Metan_analysis <- read_excel("Metan_analysis.xlsx",
col_types = c("text", "text", "text",
"numeric", "numeric", "numeric",
"numeric", "numeric", "numeric",
"numeric", "numeric", "numeric",
"numeric"))
View(Metan_analysis)
Metan_analysis$ENV <- as.factor(Metan_analysis$ENV)
Metan_analysis$GEN <- as.factor(Metan_analysis$GEN)
Metan_analysis$REP<- as.factor(Metan_analysis$REP)
inspect(Metan_analysis)
#> # A tibble: 13 x 10
#> Variable Class Missing Levels Valid_n Min Median Max Outlier Text
#> <chr> <chr> <chr> <chr> <int> <dbl> <dbl> <dbl> <dbl> <lgl>
#> 1 ENV factor No 3 252 NA NA NA NA NA
#> 2 GEN factor No 28 252 NA NA NA NA NA
#> 3 REP factor No 3 252 NA NA NA NA NA
#> 4 DF numeric No - 252 62 75 91 0 NA
#> 5 FLBL numeric No - 252 24.2 39.0 56.3 2 NA
#> 6 FLBW numeric No - 252 0.37 0.92 1.63 0 NA
#> 7 PL numeric No - 252 17.1 29.9 41.9 1 NA
#> 8 PH numeric No - 252 66.5 92.6 147 0 NA
#> 9 FL numeric No - 252 3.07 5.57 10.7 1 NA
#> 10 FW numeric No - 252 0.27 0.87 1.6 0 NA
#> 11 EHL numeric No - 252 3.1 6.53 11.9 0 NA
#> 12 FNME numeric No - 252 2.67 6.67 11.3 0 NA
#> 13 Y numeric No - 252 0.25 1.22 2.61 0 NA
#> Warning: Possible outliers in variable(s) FLBL, PL, FL. Use 'find_outliers()'
#> for more details.
stab <- ge_stats(Metan_analysis, ENV, GEN, REP)
#> Error in rowSums((gamma.n)^2): 'x' must be an array of at least two dimensions Created on 2023-02-03 with reprex v2.0.2 |
@nivelive In my side, with the example data library(metan)
#> Registered S3 method overwritten by 'GGally':
#> method from
#> +.gg ggplot2
#> |=========================================================|
#> | Multi-Environment Trial Analysis (metan) v1.17.0 |
#> | Author: Tiago Olivoto |
#> | Type 'citation('metan')' to know how to cite metan |
#> | Type 'vignette('metan_start')' for a short tutorial |
#> | Visit 'https://bit.ly/pkgmetan' for a complete tutorial |
#> |=========================================================|
args(ge_stats)
#> function (.data, env, gen, rep, resp, verbose = TRUE, prob = 0.05)
#> NULL
# by default, all numeric variables are analyzed.
# So, the argument 'resp' is not mandatory
model <- ge_stats(data_ge, ENV, GEN, REP)
#> Evaluating trait GY |====================== | 50% 00:00:09 Evaluating trait HM |============================================| 100% 00:00:20 Created on 2023-02-08 with reprex v2.0.2 |
I tried making it into a reproducible example using reprex() but I am not understanding why is it not working. can you please tell me what mistake I m doing so that I can send you the proper reproducible example. I have referred Jenifer Bryan video and as per her instructions I tried to reprex it. |
setwd("D:/PhD/Ragi")
library(metan)
#> Registered S3 method overwritten by 'GGally':
#> method from
#> +.gg ggplot2
#> |=========================================================|
#> | Multi-Environment Trial Analysis (metan) v1.17.0 |
#> | Author: Tiago Olivoto |
#> | Type 'citation('metan')' to know how to cite metan |
#> | Type 'vignette('metan_start')' for a short tutorial |
#> | Visit 'https://bit.ly/pkgmetan' for a complete tutorial |
#> |=========================================================|
library(readxl)
Metan_analysis <- read_excel("Metan_analysis.xlsx",
col_types = c("text", "text", "text",
"numeric", "numeric", "numeric",
"numeric", "numeric", "numeric",
"numeric", "numeric", "numeric",
"numeric"))
Metan_analysis$REP <- as.factor(Metan_analysis$REP)
Metan_analysis$GEN <- as.factor(Metan_analysis$GEN)
Metan_analysis$ENV <- as.factor(Metan_analysis$ENV)
inspect(Metan_analysis, plot = TRUE, threshold = 28)
#> # A tibble: 13 x 10
#> Variable Class Missing Levels Valid_n Min Median Max Outlier Text
#> <chr> <chr> <chr> <chr> <int> <dbl> <dbl> <dbl> <dbl> <lgl>
#> 1 ENV factor No 3 252 NA NA NA NA NA
#> 2 GEN factor No 28 252 NA NA NA NA NA
#> 3 REP factor No 3 252 NA NA NA NA NA
#> 4 DF numeric No - 252 62 75 91 0 NA
#> 5 FLBL numeric No - 252 24.2 39.0 56.3 2 NA
#> 6 FLBW numeric No - 252 0.37 0.92 1.63 0 NA
#> 7 PL numeric No - 252 17.1 29.9 41.9 1 NA
#> 8 PH numeric No - 252 66.5 92.6 147 0 NA
#> 9 FL numeric No - 252 3.07 5.57 10.7 1 NA
#> 10 FW numeric No - 252 0.27 0.87 1.6 0 NA
#> 11 EHL numeric No - 252 3.1 6.53 11.9 0 NA
#> 12 FNME numeric No - 252 2.67 6.67 11.3 0 NA
#> 13 Y numeric No - 252 0.25 1.22 2.61 0 NA
#> Warning: Possible outliers in variable(s) FLBL, PL, FL. Use 'find_outliers()'
#> for more details. descstat <- desc_stat(Metan_analysis, stats = "all")
anova<- anova_ind(Metan_analysis, env= ENV, gen = GEN, rep = REP)
#> Evaluating trait DF |==== | 10% 00:00:00 Evaluating trait FLBL |======== | 20% 00:00:00 Evaluating trait FLBW |============= | 30% 00:00:00 Evaluating trait PL |================== | 40% 00:00:00 Evaluating trait PH |====================== | 50% 00:00:00 Evaluating trait FL |========================== | 60% 00:00:00 Evaluating trait FW |=============================== | 70% 00:00:00 Evaluating trait EHL |================================== | 80% 00:00:00 Evaluating trait FNME |====================================== | 90% 00:00:00 Evaluating trait Y |=============================================| 100% 00:00:00
anova_joint <- anova_joint(Metan_analysis, env= ENV, gen = GEN, rep = REP)
#> variable DF
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 11353.7 5676.86 1788.21 3.83e-111
#> REP(ENV) 6.00 21.7 3.62 1.14 3.42e-01
#> GEN 27.00 3069.6 113.69 35.81 8.47e-55
#> GEN:ENV 54.00 753.8 13.96 4.40 1.59e-13
#> Residuals 162.00 514.3 3.17 NA NA
#> CV(%) 2.33 NA NA NA NA
#> MSR+/MSR- 14.87 NA NA NA NA
#> OVmean 76.38 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable FLBL
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 1114 557.1 38.14 2.67e-14
#> REP(ENV) 6.00 612 101.9 6.98 1.29e-06
#> GEN 27.00 2539 94.1 6.44 7.87e-15
#> GEN:ENV 54.00 957 17.7 1.21 1.79e-01
#> Residuals 162.00 2366 14.6 NA NA
#> CV(%) 9.90 NA NA NA NA
#> MSR+/MSR- 1.86 NA NA NA NA
#> OVmean 38.59 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable FLBW
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.000 10.066 5.033 360.46 2.24e-60
#> REP(ENV) 6.000 0.622 0.104 7.43 4.88e-07
#> GEN 27.000 5.356 0.198 14.21 2.84e-30
#> GEN:ENV 54.000 2.914 0.054 3.86 1.71e-11
#> Residuals 162.000 2.262 0.014 NA NA
#> CV(%) 12.771 NA NA NA NA
#> MSR+/MSR- 2.267 NA NA NA NA
#> OVmean 0.925 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable PL
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 2296.2 1148.12 194.134 9.64e-44
#> REP(ENV) 6.00 21.1 3.52 0.595 7.34e-01
#> GEN 27.00 722.3 26.75 4.524 6.31e-10
#> GEN:ENV 54.00 674.8 12.50 2.113 1.71e-04
#> Residuals 162.00 958.1 5.91 NA NA
#> CV(%) 8.25 NA NA NA NA
#> MSR+/MSR- 3.10 NA NA NA NA
#> OVmean 29.46 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable PH
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 60360 30180.0 508.06 1.60e-70
#> REP(ENV) 6.00 1509 251.4 4.23 5.50e-04
#> GEN 27.00 5466 202.4 3.41 7.05e-07
#> GEN:ENV 54.00 3218 59.6 1.00 4.80e-01
#> Residuals 162.00 9623 59.4 NA NA
#> CV(%) 7.87 NA NA NA NA
#> MSR+/MSR- 2.51 NA NA NA NA
#> OVmean 97.94 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable FL
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 343.54 171.770 500.85 4.34e-70
#> REP(ENV) 6.00 6.07 1.012 2.95 9.23e-03
#> GEN 27.00 198.95 7.368 21.48 1.58e-40
#> GEN:ENV 54.00 37.81 0.700 2.04 3.23e-04
#> Residuals 162.00 55.56 0.343 NA NA
#> CV(%) 9.96 NA NA NA NA
#> MSR+/MSR- 2.14 NA NA NA NA
#> OVmean 5.88 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable FW
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.000 10.207 5.1033 378.52 8.73e-62
#> REP(ENV) 6.000 0.492 0.0820 6.08 9.11e-06
#> GEN 27.000 2.399 0.0888 6.59 3.41e-15
#> GEN:ENV 54.000 1.870 0.0346 2.57 2.63e-06
#> Residuals 162.000 2.184 0.0135 NA NA
#> CV(%) 14.450 NA NA NA NA
#> MSR+/MSR- 3.611 NA NA NA NA
#> OVmean 0.804 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable EHL
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 802.870 401.435 845.198 1.92e-86
#> REP(ENV) 6.00 0.742 0.124 0.261 9.54e-01
#> GEN 27.00 262.922 9.738 20.502 2.65e-39
#> GEN:ENV 54.00 77.340 1.432 3.015 4.06e-08
#> Residuals 162.00 76.943 0.475 NA NA
#> CV(%) 10.12 NA NA NA NA
#> MSR+/MSR- 2.63 NA NA NA NA
#> OVmean 6.81 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable FNME
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 416.49 208.244 353.63 7.93e-60
#> REP(ENV) 6.00 6.23 1.039 1.76 1.10e-01
#> GEN 27.00 62.95 2.331 3.96 2.14e-08
#> GEN:ENV 54.00 68.45 1.268 2.15 1.20e-04
#> Residuals 162.00 95.40 0.589 NA NA
#> CV(%) 11.61 NA NA NA NA
#> MSR+/MSR- 2.44 NA NA NA NA
#> OVmean 6.61 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> variable Y
#> ---------------------------------------------------------------------------
#> Joint ANOVA table
#> ---------------------------------------------------------------------------
#> Source Df Sum Sq Mean Sq F value Pr(>F)
#> ENV 2.00 35.90 17.9503 315.21 1.43e-56
#> REP(ENV) 6.00 1.90 0.3168 5.56 2.87e-05
#> GEN 27.00 13.28 0.4919 8.64 7.29e-20
#> GEN:ENV 54.00 7.14 0.1323 2.32 2.55e-05
#> Residuals 162.00 9.23 0.0569 NA NA
#> CV(%) 18.60 NA NA NA NA
#> MSR+/MSR- 1.98 NA NA NA NA
#> OVmean 1.28 NA NA NA NA
#> ---------------------------------------------------------------------------
#>
#> ---------------------------------------------------------------------------
#> Variables with nonsignificant GxE interaction
#> FLBL PH
#> ---------------------------------------------------------------------------
#> Done!
stab <- ge_stats(Metan_analysis, env= ENV, gen = GEN, rep = REP)
#> Error in rowSums((gamma.n)^2): 'x' must be an array of at least two dimensions Created on 2023-02-08 with reprex v2.0.2 |
In mixed-effect models (e.g., WAASB), traits with p-values equal to 1 for the GEI effects can generate such an error. I suggest you try to run one trait at once and see which one generates the error. It seems that such an error is being caused by a trait with non-significant GEI effects (try removing FLBL and PH) and run again. Since I don't have the raw data to check this out, It is difficult to say the exact source of the error. |
as per your suggestion I analyzed each trait separately and I could get results for only two traits out of 10. Is this means other traits have non significant GXE interaction? |
@nivelive If GXE interaction for one trait is 'highly' non-significant (p-value > 0.8, for example) it means that the response of genotypes is very likely to be not dependent on the environmental condition, but this must be interpreted with caution. For example, within 60-70 genotypes, a few (say, 2-3) may have different responses and interact with environments. So, it is always a good choice, to explore the dataset. Since I don't have the raw data to find the source of the error and this seems to be not a clear bug, I'm closing this now. Feel free to re-open it if you find it necessary. |
Created on 2023-02-01 with reprex v2.0.2
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