Non float32 support not implemented yet

[ljamesherald]

Test .fcs file works fine, but whhen replaced wit my .fcs files,
flowrun = load(raw"C:\Users\ljame\Documents\GitHub\FCS-with-Julia\FCS_data\BD-FACS-Aria-II.fcs")
I get ther error Non float32 support not implemented yet. Please see github issues for this project. Is there a workaround for this issue?

[tlnagy]

Can you send me a FCS file (either upload to this issue or via email) that causes this error?

FCSFiles.jl/src/parse.jl

Line 63 in adc97eb

(text_mappings["\$DATATYPE"] != "F") && error("Non float32 support not implemented yet. Please see github issues for this project.")

It looks like I only implemented support for reading Float32 values for the $DataType. From the spec it looks like there are 4 options:

This keyword describes the type of data written in the DATA segment of the data set. The four
allowed values are 'I', 'F', 'D', or 'A'. The DATA segment is a continuous bit stream with no
delimiters. 'I' stands for unsigned binary integer, F stands for single precision IEEE floating point,
'D' stands for double precision IEEE floating point, and 'A' stands for ASCII. The additional
keywords $PnB (bits per parameter) and $PnR (range per parameter) are needed to completely
describe an event in the DATA segment.

It doesn't appear to be too difficult to add support for these other datatypes.

[ljamesherald]

Sure thing, here is a link to the .fcs file. Unfortunately, GitHub doesn't allow for .fcs files to be uploaded directly.
https://drive.google.com/file/d/1AixgKRw6nFqAmjK9um1AR0mrDg83wiOO/view?usp=sharing

[tlnagy]

It looks like your file stores data as an unsigned binary integer (I) , which is not currently implemented.

I can add this feature, but I would also be happy to review and merge a PR if you want to add support. The relevant function is linked to in my previous comment. It doesn't look too difficult to do, here's the relevant section in the spec:

$DATATYPE/I/ means that the events are written as unsigned binary integers. For each
parameter in an event, both the maximum length in bits allocated for storage of the parameter
and the actual integer range used by the parameter within that allocation are needed. The
number of bits per parameter is specified by $PnB. For example, $P1B/16/ specifies that 16 bits
are allocated for parameter 1. $P1R/1024/ specifies that parameter 1 values range from 0 to
1023. This allows the data word length to be specified, facilitating compatibility between machines
with different data word lengths and enabling bit compression of the data.

[tlnagy]

Here's a dump of all the parameters from your FCS file:

 "\$P7G" => "1.0000000000"
 "\$BEGINANALYSIS" => "00000000000000"
 "\$BTIM" => "10:21:18"
 "\$P4S" => "488-FSC"
 "\$ENDSTEXT" => "00000000000000"
 "\$P4N" => "FSC1-Area"
 "\$TIMESTEP" => "0.0000000100"
 "\$FIL" => "D:\\Data\\Alberts\\Apr 23 2014\\U DMSO.fcs"
 "\$P7F" => "SSC"
 "\$P2R" => "4294967296"
 "\$P6S" => "488-SSC"
 "\$P8V" => "297.0000000000"
 "\$P1E" => "0.0,0.0"
 "\$P1G" => "1.0000000000"
 "\$P2G" => "1.0000000000"
 "\$PAR" => "10"
 "\$P4R" => "4294967296"
 "\$P4L" => "488.0000000000"
 "\$P10P" => "0"
 "\$P9G" => "1.0000000000"
 "\$P8G" => "1.0000000000"
 "\$P2P" => "0"
 "\$SMNO" => "2"
 "\$P10E" => "0.0,0.0"
 "\$P10B" => "32"
 "\$P9N" => "FL11-Height"
 "\$P6V" => "297.0000000000"
 "\$P3E" => "0.0,0.0"
 "\$P10F" => "576/21"
 "\$P3S" => "488-FSC"
 "\$P2B" => "32"
 "\$P2S" => "Time MSW"
 "\$P5B" => "32"
 "\$P5S" => "488-FSC"
 "\$P7L" => "488.0000000000"
 "\$LOST" => "0"
 "\$P5E" => "0.0,0.0"
 "\$DATE" => "23 Apr 2014"
 "\$P7N" => "SSC1-Area"
 "\$TR" => "SSC1-Height,65535"
 "\$P3L" => "488.0000000000"
 "\$NEXTDATA" => "00000000000000"
 "\$P1P" => "0"
 "\$P3N" => "FSC1-Height"
 "\$P2N" => "TIME2"
 "\$P4F" => "FSC"
 "\$P3B" => "32"
 "\$P10N" => "FL12-Height"
 "\$ETIM" => "10:22:34"
 "\$P5G" => "6.0000000000"
 "\$P9S" => "DiO 488-513/26"
 "\$P1S" => "Time LSW"
 "\$P7V" => "297.0000000000"
 "\$P4G" => "1.0000000000"
 "\$P8N" => "SSC1-Width"
 "\$P6P" => "0"
 "\$P9E" => "0.0,0.0"
 "\$P6L" => "488.0000000000"
 "\$P9V" => "378.0000000000"
 "\$P6B" => "32"
 "\$P9P" => "0"
 "\$P3F" => "FSC"
 "\$P10G" => "1.0000000000"
 "\$SYS" => "Summit V6.2.3.15613 Released Version / Windows NT Version 6.1 / Intel Processor"
 "\$P5F" => "FSC"
 "\$P5N" => "FSC1-Width"
 "\$P6N" => "SSC1-Height"
 "\$P2E" => "0.0,0.0"
 "\$P8E" => "0.0,0.0"
 "\$TOT" => "24599"
 "\$P1B" => "32"
 "\$P8F" => "SSC"
 "\$OP" => "MoFlo Astrios"
 "\$P6F" => "SSC"
 "\$P1N" => "TIME"
 "\$P4B" => "32"
 "\$P7S" => "488-SSC"
 "\$P7R" => "4294967296"
 "\$P7E" => "0.0,0.0"
 "\$P8R" => "4294967296"
 "\$P8P" => "0"
 "\$ENDANALYSIS" => "00000000000000"
 "\$P10L" => "488.0000000000"
 "\$P4P" => "0"
 "\$P4V" => "0.0000000000"
 "\$SRC" => " "
 "\$P8B" => "32"
 "\$CYT" => "MoFlo Astrios"
 "\$SPILLOVER" => "2,9,10,1.000000,0.223429,0.028000,1.000000"
 "\$P3P" => "0"
 "\$P5R" => "4294967296"
 "\$P3G" => "6.0000000000"
 "\$P3V" => "0.0000000000"
 "\$P5L" => "488.0000000000"
 "\$P6E" => "0.0,0.0"
 "\$P7B" => "32"
 "\$INST" => "Institution"
 "\$P10R" => "4294967296"
 "\$P8S" => "488-SSC"
 "\$P9B" => "32"
 "\$P1R" => "4294967296"
 "\$PROJ" => "Project"
 "\$P10S" => "DiI 488-576/21"
 "\$P5V" => "0.0000000000"
 "\$P6R" => "4294967296"
 "\$ENDDATA" => "00000000988055"
 "\$BYTEORD" => "1,2,3,4"
 "\$P10V" => "373.0000000000"
 "\$DATATYPE" => "I"
 "\$P8L" => "488.0000000000"
 "\$BEGINSTEXT" => "00000000000000"
 "\$P4E" => "0.0,0.0"
 "\$P9R" => "4294967296"
 "\$P9F" => "513/26"
 "\$P6G" => "1.0000000000"
 "\$BEGINDATA" => "00000000004096"
 "\$P5P" => "0"
 "\$P7P" => "0"
 "\$P9L" => "488.0000000000"
 "\$P3R" => "4294967296"
 "\$MODE" => "L"