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raw-data-for-figures
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raw-data-for-figures
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Average-free SD Group
57.75050287 8.856311915 PE
40.38101575 3.015927616 GE
31.28119263 11.23726494 FE
34.97940973 7.969778948 OE
19.81694675 2.964636657 LE
43.4924343 8.424785208 Control
Body weight
Control Cd LE Cd+LE
0 22.13 24.3 23.8 23.21 21.24 20.36 22.98 24.23 23.32 24.65 23.96 22.92 23.34 22.9 24.2 22.2 23.72 23.18 23.54 22.45 22.26 22.6 21.4 20.32 23.85 24.61 23.19 24.14 21.9 23.14 22.23 21.33
4 22.3 23.41 23.6 23 20.5 19.35 22.12 22.72 24.92 23.78 24.96 22.88 22.94 22.22 23.78 22.48 24.52 22.93 23.15 22.36 23.22 21.95 21.8 19.02 23.62 24.76 22.3 24.33 22.46 24.04 21.8 21.3
8 22.9 24.11 25.01 23.42 22.26 19.51 23.05 23.14 26.2 24.65 23.72 23.83 23.65 23.05 23.68 22.72 23.66 23.66 23.8 22.94 23.64 21.6 21.9 20.3 24.14 25.22 22.43 23.89 23.91 24.4 22.32 22.98
12 23.94 25.76 25.6 24.54 23.07 20.35 23.65 23.77 26.3 24.94 24.5 24.39 24.04 23.18 24.83 23.35 24.77 24.57 24.19 23.86 25.2 21.66 22.05 21.08 24.26 26.09 23.11 24.67 23.81 25.17 23.02 23.05
16 22.03 23.84 23.57 23.78 21.86 20.07 22.18 23.3 25.84 25.13 24.52 24.9 24.3 23.4 24 23.26 24.14 24.24 23.54 22.72 24.48 21.92 21.02 19.94 24.78 26.2 22.78 24.64 23.6 25.2 23.45 23.32
20 22.26 24.9 24.32 23.18 22.93 19.6 22.7 23.21 26.12 25.49 24.42 25.1 24.3 23.18 23.98 22.7 25.72 24.68 24.4 23.48 24.93 22.03 21.59 19.94 25.03 26.89 23.71 24.12 23.58 24.9 23.65 23.4
24 23.06 25.6 25.09 23.76 23.33 19.72 23.26 24.74 25.46 26.05 25.16 25.91 25.05 23.84 24.95 23.14 25.88 24.55 24.73 23.92 25.36 22.54 21.92 20.4 25 26.71 23.59 24.08 23.9 26.06 23.73 24.2
28 22.02 25.09 24.43 23.3 23.05 22.4 22.74 25.3 26.12 26.25 25.84 25.52 24.89 24.39 24.57 23.7 26.36 25.28 24.7 24.39 23.8 23.08 22.16 19.66 25.06 26.66 22.75 23.33 24.07 25.81 24.08 24.58
Body weight gain
Control Cd LE Cd+LE
-0.49706 3.251029 2.647059 0.387764 8.521657 10.01965 -1.04439 4.416013 12.00686 6.490872 7.846411 11.3438 6.64096 6.50655 1.528926 6.756757 11.12985 9.059534 4.927782 8.641425 6.918239 2.123894 3.551402 -3.24803 5.073375 8.329947 -1.89737 -3.35543 9.908676 11.53846 8.322087 15.23676
Food intake
Control Cd LE Cd+LE
145.89 146.87 153.72 140.21 150.47 134.05 145.75 136.72 150.38 156.25 159.46 153.62 161.81 148.2 163.64 144.59 136.48 135.73 151.06 138.96 141.05 126.49 136.29 134.72 156.74 157.27 159.59 167.3 158.09 160.44 142.94 145.02
Day 21 blood Cd
Cd Cd+LE
20.66487 34.45642 20.14376 14.99551 31.8239 25.09434 17.25067 17.65499 22.99191 4.959569 20.16173 7.637017
Day 28 blood Cd
Cd Cd+LE
16.8823 61.34771 60.72776 64.61815 28.57143 49.50225 8.283917 17.92453 20.05391 46.1186 26.07098 14.40341
Heart Cd
Cd Cd+LE
0.726404 1.047345 1.158621 1.5 0.932143 2.546053 0.047059 0.399419 0.726786 0.272535 0.952083 0.233824
Lung Cd
Cd Cd+LE
0.6375 0.805556 0.73328 0.35909 0.87344 0.66398 0.251786 0.425 0.39761 0.40023 0.36244 0.60823
Liver Cd
Cd Cd+LE
4.262 1.38 4.813 9.265 3.455 2.423 6.156 15.276 9.462 6.436 8.589 9.037
Kidney Cd
Cd Cd+LE
7.008 10.87261 9.376344 10.93985 7.13099 6.043636 9.272727 11.48498 10.82143 9.110429 12.42222 10.19802
CCK licorice
0 1 110.6092 107.221 95.44717 93.88014 99.72471 93.11781
25 2 111.9221 123.4841 117.0043 114.082 118.9525 113.0232
50 3 132.971 136.4015 125.517 127.9311 108.9998 104.6375
100 4 112.1338 110.1433 106.8822 120.0113 111.1174 117.4278
200 5 127.7193 124.5853 123.7806 125.9406 109.2539 114.8444
400 6 104.3411 107.221 109.0845 114.5055 112.9809 116.962
800 7 115.3102 115.9032 115.4796 120.6889 100.2753 102.1811
1,600 8 111.8797 113.1079 107.8563 107.5175 105.6116 116.5384
3,200 9 128.7781 125.6865 129.2017 122.9336 118.9948 119.2913
6,400 10 117.0467 122.383 121.9171 126.8723 123.1877 124.0771
12,800 11 74.56766 81.30162 72.95829 70.07836 51.57056 52.75641
25,600 12 4.094035 1.552919 0.959991 4.305795 3.712867 3.246996
CCK Cd
0
0 98.791 106.6101 98.41418 97.66053 99.68596 98.83811
0.049 123.9441 119.799 120.5998 116.125 116.9728 112.922
0.098 110.9907 109.0595 102.2766 101.523 100.3454 100.2041
0.195 122.6252 117.5381 112.2154 111.4618 104.1608 112.7335
0.391 106.3275 108.7769 101.994 99.49755 100.0157 102.2766
0.781 127.0058 116.4076 114.9474 117.1612 111.6031 114.3821
1.563 112.2154 109.0124 112.2625 111.2733 104.8202 105.7623
3.125 125.1688 116.7373 109.1537 109.1537 114.2879 109.9545
6.250 111.8386 114.335 113.5814 103.5484 109.766 112.8277
12.500 87.72176 86.49708 91.77263 91.2074 95.7764 95.58799
25.000 16.83151 16.73731 9.153698 11.13203 4.396279 4.631795
50.000 1.381677 1.146161 1.193264 1.146161 0.957748 1.004852
Cell culture medium
Cd Cd+LE
12.63 12.15 11.755 11.075 10.09 10.135
Cell
Cd Cd+LE
132.9 138.5 118.2 171.9 145.3 161.3
Liver Mt1
Control LE Cd Cd+LE
1 11.17784 3.560891 7.404359123
2.309266 5.227146 2.254071 10.90221943
2.503938 1.292897 2.826807 6.499212689
1.179141 3.772146 3.018015 18.27369084
1.460388 3.39166 2.911392 25.56559954
2.670181 1.475093 4.095863 18.21019031
Hepatic cell Mt1
Control Glycyrrhizin Enoxolone
0.6 0.613848 0.741648
0.58585 0.7843 0.8601
0.61824 0.727038 0.817271
18heatmap
Cd 1 Cd 2 Cd 3 Cd+LE 1 Cd+LE 2 Cd+LE 3
s_Parabacteroides distasonis 5.976202 5.702279 5.10474 6.670851 6.8359 6.698588
s_Bacteroides 2_1_33B 5.289018 4.88544 4.32842 6.057985 6.220537 6.074148
s_Parabacteroides D26 4.886637 4.568648 3.935608 5.643338 5.819142 5.673281
s_Parabacteroides D13 4.862143 4.484983 3.895975 5.599848 5.767012 5.632674
s_Parabacteroides CAG:2 4.703687 4.321308 3.750508 5.50118 5.666978 5.537985
s_Bacteroides 3_1_19 4.621882 4.307239 3.649919 5.395351 5.555825 5.421061
s_Parabacteroides 20_3 4.527785 4.638629 3.813981 5.087064 5.235079 5.122347
s_Porphyromonas 31_2 4.273557 3.925518 3.321805 5.036964 5.207187 5.082879
s_Bacteroides faecichinchillae 4.522887 4.098436 3.460898 4.528248 4.810206 4.744637
s_Parabacteroides gordonii 4.367915 3.900476 3.280578 4.444732 4.793707 4.65485
s_Parabacteroides D25 4.221936 4.33694 3.594393 4.599839 4.726059 4.591042
s_Rikenella microfusus 4.117271 3.624901 3.087071 4.486119 4.675613 4.529456
s_Acholeplasma CAG:878 4.13456 4.240549 4.518856 3.123852 3.002598 3.184691
s_Clostridium neopropionicum 4.007662 4.165363 4.1712 3.788451 3.500785 3.714665
s_Oscillibacter KLE 1728 4.297761 4.069742 4.31618 3.892651 3.52009 3.929112
s_Clostridium propionicum 4.222872 4.345178 4.354493 4.108497 3.6787 3.89176
s_Oscillibacter CAG:155 4.915326 4.714196 4.939679 4.563339 4.11294 4.522575
s_Butyricicoccus pullicaecorum 5.009493 5.176381 5.244955 4.622649 4.135705 4.591621
PcoA
Cd Cd+LE Control LE
Cd_1 0.067311 0.249364
Cd_2 -0.19734 0.126867
Cd_3 -0.41061 -0.10527
Cd+LE_1 -0.08154 -0.16195
Cd+LE_2 0.337207 -0.05115
Cd+LE_3 0.226769 -0.0286
Con_1 -0.10674 0.114536
Con_2 -0.31492 -0.07674
Con_3 -0.22593 0.039943
LE_1 0.269594 -0.07774
LE_2 0.205112 -0.01386
LE_3 0.231094 -0.0154
Shannon
Control LE Cd Cd+LE
5.089203 4.525804 5.358692 4.941825
5.311726 4.46421 4.964296 4.176015
5.358692 4.29513 4.800335 4.646607
LEfSe NR
s_Parabacteroides sp. CAG:2 13 -3.49788888974
s_Parabacteroides sp. D13 12 -3.62423902048
s_Parabacteroides sp. D26 11 -3.63877808092
s_Bacteroides sp. 2_1_33B 10 -4.02733825871
g_unclassified_o_Bacteroidales 9 -4.63190834859
s_Parabacteroides distasonis 8 -4.63873757288
f_unclassified_o_Bacteroidales 7 -4.64095862023
s_unclassified_o_Bacteroidales 6 -4.66641322861
g_Parabacteroides 5 -4.87103837294
f_Porphyromonadaceae 4 -4.90902314877
s_Oscillibacter sp. 1-3 3 3.52114896073
g_Oscillibacter 2 3.72991347489
f_Oscillospiraceae 1 3.76185708225
KEGG pathways
Cd+LE Cd
Naphthalene degradation 0.01151 0.005081 3 0.02276 0.004066 3
Biosynthesis of vancomycin group antibiotics 0.04909 0.007304 3 0.06678 0.001446 3
Nitrotoluene degradation 0.05948 0.015 3 0.1056 0.02326 3
Primary bile acid biosynthesis 0.1248 0.02051 3 0.07013 0.0117 3
Secondary bile acid biosynthesis 0.1286 0.0197 3 0.08435 0.002384 3
Novobiocin biosynthesis 0.1685 0.009896 3 0.1932 0.01043 3
Neomycin, kanamycin and gentamicin biosynthesis 0.2147 0.03042 3 0.1287 0.006854 3
Polyketide sugar unit biosynthesis 0.2917 0.006244 3 0.3194 0.006493 3
Selenocompound metabolism 0.359 0.0132 3 0.3874 0.005735 3
Monobactam biosynthesis 0.3775 0.00517 3 0.3528 0.008837 3
Nitrogen metabolism 0.4743 0.0167 3 0.4207 0.02102 3
Porphyrin and chlorophyll metabolism 1.035 0.01022 3 0.9006 0.02997 3
Methane metabolism 1.088 0.04414 3 1.197 0.01259 3
2-Oxocarboxylic acid metabolism 1.112 0.02644 3 1.061 0.01798 3
CAZy GH
Cd+LE Cd
GH43_8 0.3463 0.06379 3 0.0269 0.02132 3
GH30 0.3913 0.09721 3 0.1052 0.06702 3
GH15 0.4008 0.0941 3 0.1169 0.05745 3
GH43_33 0.4497 0.06757 3 0.09149 0.03012 3
GH43_10 0.4535 0.1087 3 0.8368 0.1997 3
GH125 0.6566 0.07485 3 0.4784 0.06089 3
GH106 0.6768 0.06098 3 1.205 0.1539 3
GH43_28 0.7003 0.09591 3 0.1954 0.06313 3
GH144 0.8125 0.1305 3 0.1997 0.1331 3
GH123 0.9266 0.02291 3 0.5714 0.07904 3
GH26 0.998 0.149 3 0.2383 0.04543 3
GH105 1.562 0.2875 3 2.804 0.4177 3
GH78 3.055 0.167 3 1.966 0.2117 3
GH73 3.253 0.07626 3 2.272 0.2043 3
GH92 5.943 0.9993 3 2.613 1.443 3
logFC KEGG pathways
Naphthalene degradation -0.98361
Biosynthesis of vancomycin group antibiotics -0.44399
Nitrotoluene degradation -0.82813
Primary bile acid biosynthesis 0.831514
Secondary bile acid biosynthesis 0.608431
Novobiocin biosynthesis -0.19735
Neomycin, kanamycin and gentamicin biosynthesis 0.73831
Polyketide sugar unit biosynthesis -0.13088
Selenocompound metabolism -0.10984
Monobactam biosynthesis 0.097626
Nitrogen metabolism 0.173008
Porphyrin and chlorophyll metabolism 0.200672
Methane metabolism -0.13774
2-Oxocarboxylic acid metabolism 0.067732
Cysteine and methionine metabolism 0.041465
LEfSe KEGG pathways
Cd+LE Cd
Biosynthesis of vancomycin group antibiotics 1 2.024369
Polyketide sugar unit biosynthesis 2 2.258343
Nitrotoluene degradation 3 2.320351
Selenocompound metabolism 4 2.416848
Methane metabolism 5 2.73378
Aminobenzoate degradation 6 2.008844
Phenazine biosynthesis 7 2.184583
Monobactam biosynthesis 8 2.204283
Secondary bile acid biosynthesis 9 2.320032
Nitrogen metabolism 10 2.350904
Primary bile acid biosynthesis 11 2.398224
Cysteine and methionine metabolism 12 2.591789
2-Oxocarboxylic acid metabolism 13 2.646709
Neomycin, kanamycin and gentamicin biosynthesis 14 2.665073
Arginine and proline metabolism 15 2.772285
Porphyrin and chlorophyll metabolism 16 2.90267
Pos PLS-DA
Cd Cd+LE Con LE
1 13.2322 35.6463
2 85.6703 40.082
3 76.8352 5.0682
4 75.7383 54.6169
5 78.4827 16.9554
6 49.7024 13.8376
1 -78.89 4.767
2 -88.1547 -6.8476
3 -74.7245 8.1235
4 -53.4732 -29.1979
5 -37.9185 -4.9102
6 -63.8253 -23.3594
1 28.6126 15.4564
2 48.0695 -46.6079
3 74.5445 -9.2128
4 52.2149 -53.5376
5 52.8885 -59.257
6 33.9362 -38.3998
1 -63.5512 3.0891
2 -65.1111 10.4636
3 5.221 4.6086
4 -46.2912 25.2178
5 -45.8924 30.089
6 -57.3161 3.3086
Neg PLS-DA
Cd Cd+LE Con LE
1 11.7246 37.5836
2 84.7362 35.1846
3 72.5912 -15.064
4 70.3401 56.6382
5 82.3108 21.1983
6 51.4932 17.6186
1 -68.8377 13.5677
2 -68.0912 13.1257
3 -80.4954 1.3346
4 -56.6633 -39.0523
5 -49.3957 -13.3281
6 -87.387 -22.6576
1 35.786 21.9277
2 58.2824 -37.3772
3 77.5813 2.1562
4 46.4871 -56.9543
5 48.9545 -70.1347
6 24.4189 -38.1582
1 -60.4848 4.8144
2 -61.2426 5.7151
3 1.467 -2.2619
4 -38.7954 25.2332
5 -53.4933 33.7692
6 -41.2869 5.1211
Diff_HMDB176
Number
Triterpenoids 32
Amino acids, peptides, and analogues 13
Fatty acids and conjugates 13
Lineolic acids and derivatives 10
Terpene lactones 6
Glycerophosphoethanolamines 6
Bile acids, alcohols and derivatives 6
Eicosanoids 6
Diterpenoids 6
Glycerophosphocholines 5
Purines and purine derivatives 4
Androstane steroids 4
Vitamin D and derivatives 4
Chalcones and dihydrochalcones 3
Quinone and hydroquinone lipids 3
Fatty acyl glycosides 3
Glycerophosphates 2
Medium-chain hydroxy acids and derivatives 2
Amines 2
Monoradylglycerols 2
Sesquiterpenoids 2
Steroid lactones 2
Flavones 2
Fatty alcohols 2
Ergostane steroids 2
Terpene glycosides 2
Delta valerolactones 2
Carbohydrates and carbohydrate conjugates 2
Others 28
Correlation
Methyl oleate L-Glutamate Styrene 13-Hydroxy-9-methoxy-10-oxo-11-octadecenoic acid 12,13-Epoxy-9,15-octadecadienoic acid Methyl linoleate PG(18:1(9Z)/0:0) 4-Imidazolone-5-propionic acid 10E,12Z-Octadecadienoic acid Traumatic Acid Carboprost Tromethamine ALANYL-dl-PHENYLALANINE 22-Hydroxy Vitamin D4 1α-hydroxy-26,27-dimethylvitamin D3 2-Hydroxydecanedioic acid Hydroxyprolyl-Proline L-Tyrosine 15(R)-Iloprost Farnesiferol B 9S-hydroxy-12R,13S-epoxy-10E,15Z-octadecadienoic acid 9,12,13-TriHOME Corchorifatty acid D 9,10,13-TriHOME Rollinecin A 2-Indolecarboxylic acid D-Pipecolic acid Cinnzeylanol 3-Hydroxydodecanedioic acid Suberic acid 7α-Hydroxydehydroepiandrosterone Polyethylene, oxidized Hypoxanthine SM(d18:1/12:0) 12,20-DiHETE DG(18:3(6Z,9Z,12Z)/18:4(6Z,9Z,12Z,15Z)/0:0) DG(18:2(9Z,12Z)/18:4(6Z,9Z,12Z,15Z)/0:0) (2S)-2-{[1-(R)-Carboxyethyl]amino}pentanoate PS(O-16:0/0:0) DG(18:2(9Z,12Z)/16:1(9Z)/0:0) LysoPC(16:0) LysoPC(18:1(11Z)) PE(18:1(9Z)/0:0) LysoPE(16:0/0:0) PE(16:0/0:0) Corosin PE(14:0/0:0) Theasapogenol E ENOXOLONE Propapyriogenin A2 Ganoderic acid beta
s_Parabacteroides_distasonis -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Bacteroides_sp._2_1_33B -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Parabacteroides_sp._D25 -0.3566 -0.3287 -0.3566 -0.3706 -0.3706 -0.4406 -0.4056 -0.3846 -0.4615 -0.4545 -0.4825 -0.4056 -0.6154 -0.6154 -0.4895 -0.5035 -0.5524 -0.5524 -0.5524 -0.5734 -0.5315 -0.6853 -0.6573 -0.6573 -0.6084 -0.6713 -0.6503 -0.5664 -0.5944 -0.7762 -0.4406 -0.2587 -0.2587 -0.2937 -0.2378 -0.2028 0.5105 0.3636 0.2378 0 0 0.2098 0.5315 0.5385 0.5804 0.6224 0.7972 0.7483 0.7413 0.6993
s_Rikenella_microfusus -0.4965 -0.4895 -0.3986 -0.3916 -0.4825 -0.6014 -0.5804 -0.5455 -0.6084 -0.5944 -0.5734 -0.5524 -0.5105 -0.5315 -0.6294 -0.6154 -0.6434 -0.6573 -0.6713 -0.6014 -0.6434 -0.6224 -0.6154 -0.5734 -0.7692 -0.7902 -0.7203 -0.7483 -0.6923 -0.7902 -0.2937 -0.4895 -0.1888 -0.2168 -0.1678 -0.042 0.5874 0.5385 0.4895 0.1469 0.1748 0.1958 0.6434 0.6503 0.6713 0.7273 0.8811 0.8112 0.7832 0.7972
s_Acholeplasma_sp._CAG:878 0.5944 0.5594 0.3916 0.8741 0.7203 0.5874 0.5385 0.5874 0.5594 0.6853 0.7203 0.7133 0.5245 0.3706 0.2727 0.7343 0.8182 0.7133 0.6923 0.3846 0.5804 0.7273 0.7133 0.6853 0.6503 0.6783 0.5455 0.5664 0.6224 0.5944 0.1049 0.1818 0.4685 0.5594 0.5594 0.2937 -0.6084 -0.6154 -0.2028 -0.3986 -0.4056 -0.6923 -0.7832 -0.8042 -0.5594 -0.7552 -0.6853 -0.7483 -0.7552 -0.7133
s_Clostridium_neopropionicum 0.4476 0.4336 0.3776 0.4126 0.3706 0.4825 0.5245 0.4196 0.3357 0.5315 0.5594 0.5315 0.3147 0.2448 0.4476 0.6224 0.6364 0.7552 0.7413 0.5524 0.4895 0.4895 0.6014 0.4056 0.7063 0.6923 0.7483 0.6853 0.6084 0.7902 0.2937 0.2657 0.3427 0.3077 0.1049 -0.2168 -0.3357 -0.5245 -0.4406 -0.1608 -0.1888 -0.1049 -0.6014 -0.6224 -0.6643 -0.7203 -0.7552 -0.8252 -0.7133 -0.7692
s_Oscillibacter_sp._KLE_1728 0.4895 0.3776 0.4056 0.5175 0.4965 0.4755 0.4406 0.4056 0.4196 0.6014 0.6573 0.5175 0.4196 0.3497 0.2308 0.6713 0.7203 0.7972 0.7413 0.6643 0.5944 0.7273 0.7552 0.6014 0.6224 0.6853 0.7832 0.5804 0.6643 0.8252 0.3077 0.1469 0.4685 0.4895 0.2587 0.0909 -0.3636 -0.4476 -0.2168 -0.2098 -0.1538 -0.3566 -0.6713 -0.6783 -0.6713 -0.7622 -0.7762 -0.8112 -0.7622 -0.7343
s_Clostridium_propionicum 0.4406 0.4196 0.3287 0.3147 0.3077 0.4825 0.5245 0.3986 0.3077 0.5035 0.5175 0.4965 0.2448 0.1888 0.4755 0.5734 0.5874 0.7133 0.7203 0.5455 0.4615 0.4196 0.5175 0.3077 0.6993 0.6923 0.7483 0.7063 0.6084 0.7902 0.2308 0.3147 0.2587 0.2168 0.014 -0.3007 -0.2867 -0.5175 -0.5035 -0.1888 -0.2308 -0.042 -0.5455 -0.5734 -0.6084 -0.6713 -0.7203 -0.7832 -0.6503 -0.7343
s_Oscillibacter_sp._CAG:155 0.3986 0.3217 0.4406 0.4965 0.4266 0.3986 0.3916 0.3357 0.3497 0.5245 0.5804 0.4545 0.4406 0.3846 0.2448 0.6434 0.6923 0.7413 0.6923 0.6434 0.5594 0.7203 0.7483 0.5804 0.6084 0.6643 0.7762 0.5594 0.6434 0.8042 0.3986 0.0979 0.4336 0.4406 0.2168 0.021 -0.3986 -0.3986 -0.1958 -0.1538 -0.1119 -0.3217 -0.6434 -0.6503 -0.6573 -0.7203 -0.7552 -0.7972 -0.7203 -0.6993
s_Butyricicoccus_pullicaecorum 0.6923 0.6783 0.3986 0.5594 0.6503 0.7063 0.7133 0.7063 0.6713 0.8042 0.8182 0.7762 0.3287 0.2517 0.5385 0.8671 0.8881 0.9021 0.8741 0.7413 0.8112 0.7413 0.7622 0.5105 0.8951 0.8881 0.7972 0.8112 0.8741 0.7972 0.1958 0.5035 0.3287 0.3427 0.0769 -0.1538 -0.4895 -0.7343 -0.5664 -0.2657 -0.2448 -0.4196 -0.8741 -0.8811 -0.7762 -0.9371 -0.9301 -0.958 -0.8951 -0.9021
s_Parabacteroides_sp._D26 -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Parabacteroides_sp._D13 -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Parabacteroides_sp._CAG:2 -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Bacteroides_sp._3_1_19 -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Parabacteroides_sp._20_3 -0.3706 -0.3497 -0.3776 -0.3427 -0.3776 -0.4545 -0.4266 -0.3846 -0.4615 -0.4615 -0.4895 -0.4196 -0.5944 -0.5874 -0.5035 -0.5455 -0.5804 -0.5804 -0.5874 -0.6154 -0.5664 -0.6923 -0.6364 -0.6154 -0.6364 -0.7063 -0.6993 -0.6084 -0.6364 -0.8042 -0.4126 -0.2937 -0.2098 -0.2448 -0.1888 -0.1189 0.5175 0.3846 0.2797 0.049 0.0559 0.2028 0.5524 0.5594 0.5944 0.6364 0.7972 0.7622 0.7133 0.6993
s_Porphyromonas_sp._31_2 -0.3776 -0.3916 -0.3776 -0.3986 -0.3916 -0.4685 -0.4685 -0.4406 -0.4965 -0.4755 -0.5035 -0.4545 -0.6084 -0.6014 -0.5664 -0.5455 -0.5874 -0.5664 -0.5734 -0.5594 -0.5594 -0.6783 -0.6503 -0.6294 -0.6643 -0.7063 -0.6434 -0.6084 -0.6224 -0.7552 -0.4406 -0.3147 -0.2238 -0.2587 -0.2168 -0.1259 0.5594 0.4266 0.3007 0 0.021 0.2028 0.5734 0.5804 0.6014 0.6503 0.8182 0.7762 0.7483 0.7273
s_Bacteroides_faecichinchillae -0.4615 -0.4895 -0.3846 -0.3916 -0.4406 -0.5734 -0.5664 -0.5175 -0.5385 -0.5385 -0.5315 -0.5524 -0.5524 -0.5315 -0.6713 -0.5944 -0.6084 -0.6294 -0.6573 -0.5524 -0.5734 -0.5874 -0.5944 -0.5664 -0.7552 -0.7622 -0.6783 -0.7273 -0.6434 -0.7832 -0.3287 -0.4406 -0.1958 -0.2168 -0.1958 -0.021 0.5664 0.5315 0.4615 0.0909 0.1469 0.1469 0.6014 0.6154 0.6503 0.6923 0.8392 0.8182 0.7692 0.7972
s_Parabacteroides_gordonii -0.4965 -0.4895 -0.3986 -0.3916 -0.4825 -0.6014 -0.5804 -0.5455 -0.6084 -0.5944 -0.5734 -0.5524 -0.5105 -0.5315 -0.6294 -0.6154 -0.6434 -0.6573 -0.6713 -0.6014 -0.6434 -0.6224 -0.6154 -0.5734 -0.7692 -0.7902 -0.7203 -0.7483 -0.6923 -0.7902 -0.2937 -0.4895 -0.1888 -0.2168 -0.1678 -0.042 0.5874 0.5385 0.4895 0.1469 0.1748 0.1958 0.6434 0.6503 0.6713 0.7273 0.8811 0.8112 0.7832 0.7972
Licorice-derived metabolites
Cd_1 Cd_2 Cd_4 Cd_3 Cd_5 Cd_6 Cd_lic_4 Cd_lic_5 Cd_lic_1 Cd_lic_6 Cd_lic_2 Cd_lic_3
ENOXOLONE 5.1618 4.8788 4.9601 4.719 4.8162 4.915 8.0671 8.1062 8.1363 8.0511 8.2187 8.1666
Cyclohexylsulfamate 3.1475 3.0458 2.9651 3.7672 3.2006 3.1878 7.1592 7.0202 7.0559 6.9468 6.7262 6.9249
Alisol C 4.2589 3.2627 4.0796 3.2372 3.5384 3.7141 6.6202 6.6783 6.7776 6.7493 6.8002 6.7258
Propapyriogenin A2 4.861 4.4548 4.4098 4.2698 4.3859 3.9943 6.5396 6.6221 6.777 6.7419 6.8298 6.6705
Glabric acid 4.0819 3.9634 3.9907 4.0361 4.179 3.746 6.4118 6.4469 6.5967 6.7 6.6377 6.4934
Uralenolide 4.1012 3.6835 3.9931 3.8682 3.362 3.8803 6.4727 6.5049 6.5183 6.3845 6.5611 6.632
(3β,19α)-3,19,23,24-Tetrahydroxy-12-oleanen-28-Oate 4.163 3.9954 3.7812 3.8978 4.1204 3.9986 6.3893 6.4396 6.3882 6.1251 6.3119 6.5379
Theasapogenol A 4.3866 3.3145 3.8117 1.4125 3.7589 3.7115 6.2041 6.2693 6.3142 6.206 6.2969 6.2924
Isoartocarpesin 2.8045 2.8837 3.3599 3.0572 2.9766 2.8332 6.0918 6.1302 6.0915 5.8737 5.9655 6.2049
Dioscoretine 2.0331 1.2024 0.4705 0.5153 0.8413 1.3498 6.0259 6.0784 6.0851 5.845 5.9997 6.1639
Amino acid metabolites
Cd_1 Cd_2 Cd_4 Cd_3 Cd_5 Cd_6 Cd_lic_4 Cd_lic_5 Cd_lic_1 Cd_lic_6 Cd_lic_2 Cd_lic_3
L-Tyrosine 6.0847 6.2345 6.0489 6.294 6.1671 6.0143 6.0783 6.0984 6.0175 6.0028 5.9294 5.9942
D-Pipecolic acid 6.0636 6.2603 6.1396 6.3627 6.2751 6.1293 6.1217 6.0618 5.9153 6.0997 5.8423 5.9024
Hydroxyprolyl-Proline 5.645 5.9252 5.7799 6.0721 5.9806 5.7869 5.6978 5.7278 5.5264 5.6579 5.5374 5.4834
N-Oleoyl phenylalanine 5.5023 5.1267 5.3574 5.1956 5.1181 5.332 5.3869 5.4709 5.5984 5.5887 5.6354 5.5835
L-Glutamate 4.9932 6.4465 6.04 6.5183 6.2991 6.0394 5.882 5.8502 5.2198 5.4664 5.2839 5.2356
Pyroglutamic acid 4.5555 5.6203 5.1803 5.613 5.6019 5.2511 5.3269 5.2001 4.601 4.8368 4.6278 4.6968
Isoleucyl-Leucine 4.6068 5.2963 5.14 5.3475 5.2693 5.1215 5.0532 5.0344 4.607 4.8546 4.6777 4.7142
Isoleucyl-Lysine 3.9812 6.0743 5.8447 6.1033 5.8076 5.7198 5.2663 5.5343 4.4638 4.4153 4.4919 4.4914
Tyrosyl-Isoleucine 4.4382 5.4913 5.2173 5.4591 5.2266 5.1336 4.8849 5.0031 4.5731 4.506 4.5324 4.5741
Cyclo(Leu-Phe) 3.9109 5.9236 5.5456 5.9265 5.6018 5.4295 5.14 5.3084 4.1778 4.5973 4.2369 4.3593
L-Glutamine 4.4711 5.0831 4.8658 5.1473 5.027 4.9913 4.74 4.7206 4.6016 4.4981 4.5062 4.4282
Glutamylphenylalanine 4.0113 4.8401 4.5626 4.8559 4.744 4.5563 4.5056 4.4767 4.1875 4.1933 4.2328 4.169
Leu-Leu-Leu 3.7732 5.0503 4.7836 5.1264 5.0013 4.7387 4.4557 4.5184 3.7997 4.3576 3.8466 4.0423
Tyrosyl-Lysine 3.6791 5.3135 5.1429 5.3776 5.0842 5.0151 4.6419 4.9332 4.0214 3.3038 3.8455 4.0136
Bile acids, alcohols and derivatives
3β,7α-Dihydroxy-5-cholestenoate 4.1523 3.9543 4.1249 4.4248 4.1484 4.1918 5.2712 5.3435 5.3548 5.5136 5.4627 5.2657
3α,7α-Dihydroxycoprostanic acid 4.8496 4.7055 4.6665 4.8551 4.6858 4.7678 5.2831 5.3251 5.3406 5.1512 5.27 5.3844
Taurochenodeoxycholate-7-sulfate 5.8019 6.0264 6.1968 5.5656 6.0599 6.0714 5.442 4.8674 5.6847 4.4444 5.7548 4.4331
(25R)-3β,4β-dihydroxycholest-5-en-26-al 5.1536 5.4838 5.4011 5.4763 5.1115 5.1001 4.9463 5.0213 5.0216 5.0134 4.9745 4.9166
(25R)-3β,4β-dihydroxycholest-5-en-26-oate(1-) 4.0067 4.14 4.121 4.2107 4.0523 3.8336 4.5004 4.5737 4.5911 4.7244 4.6097 4.4507
Tauroursodeoxycholic acid 4.1788 4.6969 4.9396 3.9837 4.6944 4.6388 3.5783 3.547 3.8548 3.701 3.8925 3.5683
14 Bas levels
Cd Cd+LE
3b3b3b3b3b 12a-Dihydroxy-5a-cholanoic 5.8878 5.4081 5.13 5.5513 5.2162 4.922 5.5324 5.5974 5.562 5.8839 5.3986 5.7805
acid 6.3365 5.6129 6.2264 5.3239 5.7115 6.0197 5.7121 5.7794 6.201 6.1109 6.4579 5.8532
-cholanoic acid 5.3474 4.7823 4.6382 5.0523 4.5425 4.2613 4.9894 5.123 5.0168 5.4928 5.0587 5.0726
7.2095 7.6598 7.8084 6.9421 7.5908 7.792 6.7784 6.1892 7.7077 5.9863 7.7962 5.7742
olanoic acid 5.7445 5.4552 5.1328 5.612 5.2501 4.9719 5.5646 5.6176 5.5508 5.7068 5.3308 5.7008
a,12b-Trihydroxy-5b-cholanoic acid 5.8019 6.0264 6.1968 5.5656 6.0599 6.0714 5.442 4.8674 5.6847 4.4444 5.7548 4.4331
3b,12α-Dihydr 5.5062 4.8545 5.47 4.1975 4.9706 5.3212 4.9934 4.9857 5.4915 5.2072 5.7226 5.0678
oxy-5a-cholanoic acid 4.8496 4.7055 4.6665 4.8551 4.6858 4.7678 5.2831 5.3251 5.3406 5.1512 5.27 5.3844
Trihydroxy-5b-cholanoic acid 4.8372 4.8332 4.456 5.158 4.6854 4.3937 4.7343 4.7647 4.2992 4.7614 4.1443 4.3367
Trihydroxy-5b-cholan 4.2245 3.9153 4.0349 4.1621 3.9698 4.3421 4.2075 4.209 4.087 3.8692 4.158 4.1965
oic acid 6.4272 4.996 4.5521 6.2746 4.9722 4.822 6.4239 6.4804 6.0627 6.5482 4.3951 6.4967
ydroxy-5a-cholanoic acid 4.1788 4.6969 4.9396 3.9837 4.6944 4.6388 3.5783 3.547 3.8548 3.701 3.8925 3.5683
α,7α,12β-Trihydroxy-5b-chol 4.0135 5.2131 5.5436 3.9014 5.2598 5.2492 3.6963 3.9333 5.0066 4.0318 4.928 3.831
anoic acid 4.8822 5.3827 5.6433 4.6254 5.3535 5.4959 4.1547 3.9222 5.3267 4.0158 5.3678 3.523
Cbh gene abundance
treatment Cd Cd+LE
bsh 0.07988 0.01638 3 0.1443 0.02527 3
hdhA 0.01486 0.01119 3 0.003969 0.001199 3
Phe and Tyr levels
Cd Cd+LE
L-Phenylalanine 6.9285 6.9934 6.8537 7.0372 6.85 6.8559 6.9237 6.9934 6.9428 6.9024 6.9049 6.859
L-Tyrosine 6.0847 6.2345 6.0489 6.294 6.1671 6.0143 6.0783 6.0984 6.0175 6.0028 5.9294 5.9942
Dopaquinone 5.0546 5.0887 5.0431 5.4907 5.5746 5.0885 5.3151 5.2423 5.1251 5.0061 5.0377 5.1352
Gentisic acid 3.6264 3.4215 3.3996 3.6003 3.587 3.5926 3.8137 3.6681 3.8036 3.5285 3.6594 3.5811
Dihydroxyphenylacetic acid 3.7254 3.4922 3.5129 3.5678 3.5633 3.7164 3.3615 3.1733 3.3249 3.1946 3.1002 3.0229
Arg and Glu levels
Cd Cd+LE
L-Arginine 3.5706 4.3069 3.618 4.6282 4.0995 3.7635 3.9792 3.8109 3.8393 3.6405 3.7801 3.6637
D-Ornithine 4.3645 5.061 4.78 5.2304 4.8868 4.8783 4.8775 4.6577 4.3356 4.5355 4.3798 4.3063
Spermidine 5.0281 5.7754 5.6618 5.6141 5.6994 5.5574 5.3034 5.4488 5.1322 5.4921 4.7736 5.0912
Citrulline 5.5716 6.2331 6.0965 6.161 6.1745 5.8527 6.1003 6.1156 5.6605 6.2068 5.8972 5.7989
N1-Acetylspermidine 4.9993 5.5625 5.6037 5.6009 5.6198 5.452 5.0771 5.1735 4.828 4.9018 4.97 4.8038
L-Glutamate 5.0932 6.4465 6.04 6.5183 6.2991 6.0394 5.882 5.8502 5.2198 5.4664 5.2839 5.2356
L-Glutamine 4.4711 5.0831 4.8658 5.1473 5.027 4.9913 4.74 4.7206 4.6016 4.4981 4.5062 4.4282
Pyroglutamic acid 4.5555 5.6203 5.1803 5.613 5.6019 5.2511 5.1169 5.2001 4.601 4.8368 4.6278 4.6968
Phe gene abundance
Cd Cd+LE
tyrB 0.001346 0.001287 3 0.000903 0.000204 3
aspB 0.04637 0.0291 3 0.07042 0.02592 3
yhdR 0.03509 0.01712 3 0.01914 0.01286 3
hisC 0.07919 0.008579 3 0.07266 0.00877 3
Arg gene abundance
arg 0.0104 0.003577 3 0.06405 0.01625 3
murI 0.09 0.01253 3 0.09793 0.01011 3
glsA 0.04338 0.01972 3 0.04727 0.01179 3
gltS 0.008351 0.003069 3 0.005333 0.003781 3
glnA 0.1254 0.00313 3 0.1379 0.001578 3
gdhA 0.05102 0.01731 3 0.0503 0.01079 3