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DAFT-seq

What is DAFT-seq?

DAFT-seq is short for "Directional Amplification-free Transcriptome sequencing". It's a PCR-free RNA-seq protocol that was

Manuscripts that use the DAFT-seq protocol and this software

Chappell et al 2019: https://www.biorxiv.org/content/10.1101/852038v2

Siegel*, Chappell* et al. 2020: https://www.biorxiv.org/content/10.1101/2020.03.20.998294v1

What does this software do?

With a bit of luck this software should be able to reproduce the analysis of the DAFT-seq data in the manuscripts linked above

Dependencies

  1. tophat version 2
  2. samtools Version: 0.1.19-44428
  3. bedtools v2.17.0