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cellconfluency

dependencies:

python-skimage
python-opencv

usage:

to test/visualize threshold of standard deviation of local brightness

	segment.py -i image.jpg -S threshold -test test.jpg

to calculate confluency

	area.sh -S threshold img1_001.jpg img2_002.jpg ....

to process time lapse experiment

edit area.make, then

	make -f area.make AREA	

See example folder:

  • AF-GAMMA: images to be analyzed
  • makefiles: location of area.make
  • image_proc: output
  • segment_thr: standard deviation threshold
  • to run:
cd example
rm -rf image_proc segment_thr
cd makefiles
make AREA

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To extract culture confluency from time lapse micrographs

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