The micropower package is designed to facilitate power estimation for microbiome studies that will be analyzed with pairwise distances (beta diversity) and PERMANOVA (a non-parametric extension of multivariate analysis of variance to a matrix of pairwise distances).
It includes:
- tools to perform distance matrix simulation to model within-group pairwise distances according to pre-specified population parameters
- a method to incorporate effects of different sizes within a simulated distance matrix
- a bootstrap method for estimating PERMANOVA power from simulated distance matrices
A detailed description of the methods underlying the micropower package has been submitted for publication. Please see: Kelly BJ, Gross R, Bittinger K, Sherrill-Mix S, Lewis JD, Collman RG, Li H, Bushman FD. Power and sample-size estimation for microbiome studies using pairwise distances and PERMANOVA.