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Competition-based screening secures the evolutionary stability of a defensive microbiome

Sarah F. Worsley 1, Tabitha M. Innocent 2,†, Neil A. Holmes 1,3,†, Mahmoud M. Al-Bassam 1, Barrie Wilkinson 3, J. Colin Murrell 4, Jacobus J. Boomsma 2*, Douglas W. Yu 1,5,6,, Matthew I. Hutchings 1,3,

1 School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk, UK NR47TJ 2 Centre for Social Evolution, Section for Ecology and Evolution, University of Copenhagen, Copenhagen, Denmark. 3 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, Norfolk, UK NR47UH 4 School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk, UK NR47TJ 5 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 650223 6 Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming Yunnan, China 650223

* Correspondence: m.hutchings@uea.ac.uk, jjboomsma@bio.ku.dk, Douglas.Yu@uea.ac.uk,