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Analysis of scarab beetle genotypes for Linck & Sheldon in prep

Scripts and data

(in scripts/ subdirectory)

abc.R: run approximate Bayesian computation for demographic inference

bedassle.R: run BEDASSLE to estimate relative impact of environment on genetic differentiation

genotype_analysis.R: handle .vcf files and prepare for plotting; run PCA etc.

quality_control.R: visualize sequencing results (readcount by spp.)

scarab_analysis_notebook.Rmd: Rmarkdown digital notebook file for all assembly and analyses

scarab_analysis_notebook.md: knit digital notebook file, with images

snp_filtering.sh: SNP filtering parameters

stats.R: calculate summary statistics and mixed models

(in data/ subdirectory)

fst_dist_species.csv: pairwise FST by geographic distance data

genotypes.df.csv: genotype PCA and sample data

growth.csv: posterior distribution of parameters from ABC "growth" model

master.theta.df: etsimtates of theta across elevation

melted.bayes.csv: Bayes factor data from ABC

melted.coancestry.df.csv: fineRADstructure results

scarab_spp_master.csv: full sample and locality data

(in dDocent/ subdirectory)

*_config.file: species-specific dDocent assembly parameters

*_rename.txt: name manipulations to meet program reqs

(in bedassle/ subdirectory)

*.Robj: BEDASSLE output for each species

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scripts and data associated w/ Linck & Sheldon in prep.

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