sh-miR Designer is a software aimed for fast and efficient design of effective RNA interference (RNAi) reagents - sh-miRs, also known as artificial miRNAs. sh-miRs are RNA particles whose structure is based on miRNA precursor pri-miRNA, but sequence interacting with transcript is changed depending on research purpose. Maintenance of structure of pri-miRNA is very important to enable cellular processing and therefore ensure functionality of artificial particles. sh-miRs delivered to cells on genetic vectors - plasmids or viral vectors - enter natural RNAi pathway and silence target mRNA. They can be used in genetic therapies and basic biomedical research.
##Documentation:
##What you need to use it?
- python in version 2.7
##How to use it? Change directory to shmir_designer, run terminal and write:
./main seq1 seq2
or
./main seq
Where seq1 and seq2 are strands in 5'-3' orientation If you use only one strand program will generate another complementary to the first.
You can also use it on our website what is much easier. Instruction video
##What if you would like to make own mfold/database server? You need to do it:
- python in version 3.3
- mfold
- postgresql
- flask
- psycopg2