BACteria in Mother Maching Analyzer (BACMMAN) is a software allowing fast and reliable automated image analysis of high-throughput 2D/3D time-series images from mother machine. Mother machine is a very popular microfluidic device allowing investigating biological processes in bacteria at the single-cell level.
BACMMAN can process this kind of data:
| Fluorescence Images | Phase-contrast Images |
|---|---|
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See the wiki for detailed information
- Example datasets are taken from: Robert et al. Mutation dynamics and fitness effects followed in single cells, Science 2018
- When using this work please cite J. Ollion, L. Robert, and M. Elez, “High-throughput detection and tracking of cells and intracellular spots in mother machine experiments” Nature Protocols, 2019.
BACMMAN is a free/libre open source software under GNU General Public License v3.0 (see license)
BACMMAN is composed of several modules:
- The core module on which depend all other modules
- The graphical user interface
- Mother machine modules: image processing modules specifically designed for analysis of mother machine experiments
- IJ1 binding integration in FIJI environment
- Headless for run in command line mode
