NF-kappaB Single-Molecule FRET Traces
There are five folders:
- free - smFRET traces of free NF-kappaB
- DNA - smFRET traces of DNA-bound NF-kappaB
- WT_IkBa - smFRET traces of wildtype IkBalpha-bound NF-kappaB
- 5xPEST_IkBa - smFRET traces of 5xPEST IKBalpha-bound NF-kappaB
- DNA_titration - smFRET traces of DNA-bound NF-kappaB with varying solution DNA concentrations
In each folder, there might be subfolders stable/ and fluctuating/, each containing traces classified as stable or fluctuating respectively.
Datapoints of each background and donor leakage corrected single-molecule trace are stored in .dat, with the three columns to be Time (sec), Donor Fluorescence (a.u.), and Acceptor Fluorescence (a.u.). Plots of individual traces are stored as .png files.